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Volume electron microscopy

open access: yesNature Reviews Methods Primers, 2022
Life exists in three dimensions, but until the turn of the century most electron microscopy methods provided only 2D image data. Recently, electron microscopy techniques capable of delving deep into the structure of cells and tissues have emerged ...
C. Peddie   +13 more
semanticscholar   +4 more sources

Data-driven electron microscopy: electron diffraction imaging of materials structural properties. [PDF]

open access: yesMicroscopy, 2021
Transmission electron diffraction is a powerful and versatile structural probe for the characterization of a broad range of materials, from nanocrystalline thin films to single crystals.
J. Zuo   +7 more
semanticscholar   +1 more source

Perspective towards atomic‐resolution imaging of two‐dimensional polymers

open access: yesSmartMat, 2021
Recent years have witnessed the rise of an emerging class of synthetic two‐dimensional (2D) materials‐2D polymers. The combination of organic chemistry and rational design of polymeric crystals has stimulated tremendous research efforts in the controlled
Haoyuan Qi, Baokun Liang, Ute Kaiser
doaj   +1 more source

EMDB—the Electron Microscopy Data Bank

open access: yesbioRxiv, 2023
The Electron Microscopy Data Bank (EMDB) is the archive of three-dimensional electron microscopy (3DEM) maps of biological specimens. As of 2021, EMDB has been managed by the Worldwide Protein Data Bank (wwPDB) as a wwPDB Core Archive.
Jack Turner
semanticscholar   +1 more source

EMPIAR: the Electron Microscopy Public Image Archive

open access: yesbioRxiv, 2022
Public archiving in structural biology is well established with the Protein Data Bank (PDB; wwPDB.org) catering for atomic models and the Electron Microscopy Data Bank (EMDB; emdb-empiar.org) for 3D reconstructions from cryo-EM experiments.
Andrii Iudin   +8 more
semanticscholar   +1 more source

Multiscale and Correlative Analytical Electron Microscopy of Extraterrestrial Minerals

open access: yesFrontiers in Astronomy and Space Sciences, 2020
This paper presents a unique correlative microscopic method for the structural characterization of extraterrestrial minerals. A fragment from the pallasite Seymchan meteorite that consists of olivine grains mixed into a metallic iron matrix with variable
Mihaela Albu   +6 more
doaj   +1 more source

A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ

open access: yesbioRxiv, 2021
Transmission electron microscopy (TEM) is a scientific research standard for producing nanometer-resolution ultrastructural images of subcellular components within cells and tissues. Mitochondria, endoplasmic reticulum (ER), lysosomes, and autophagosomes
Jacob Lam   +17 more
semanticscholar   +1 more source

Precise targeting for 3D cryo-correlative light and electron microscopy volume imaging of tissues using a FinderTOP

open access: yesCommunications Biology, 2023
Cryo-correlative light and electron microscopy (cryoCLEM) is a powerful strategy to high resolution imaging in the unperturbed hydrated state. In this approach fluorescence microscopy aids localizing the area of interest, and cryogenic focused ion beam ...
Marit de Beer   +7 more
doaj   +1 more source

Vaccinia virus H7-protein is required for the organization of the viral scaffold protein into hexamers

open access: yesScientific Reports, 2022
Viruses of the giant virus family are characterized by a structurally conserved scaffold-capsid protein that shapes the icosahedral virion. The vaccinia virus (VACV) scaffold protein D13, however, transiently shapes the newly assembled viral membrane in ...
Susanne Tonnemacher   +8 more
doaj   +1 more source

Electron Microscopy [PDF]

open access: yes, 2012
Correlative light (LM) and transmission electron microscopic (TEM) analysis is useful, if ultrastructural details of cells need to be related to functional aspects which can only be examined at the LM level. The first protocol presented here introduces a relatively simple way of obtaining TEM images which, on the one hand, reveal ultrastructural ...
Luder, H U, Amstad-Jossi, M
openaire   +2 more sources

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