Results 91 to 100 of about 5,316,321 (325)

Cryo-electron microscopy structure of a coronavirus spike glycoprotein trimer

Nature, 2016
The tremendous pandemic potential of coronaviruses was demonstrated twice in the past few decades by two global outbreaks of deadly pneumonia. Entry of coronaviruses into cells is mediated by the transmembrane spike glycoprotein S, which forms a trimer ...
A. Walls, M. Tortorici, B. Bosch, Brandon Frenz, P. Rottier, F. DiMaio, Félix A Rey, D. Veesler   +7 more
semanticscholar   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Structure of the TRPV1 ion channel determined by electron cryo-microscopy

Nature, 2013
Transient receptor potential (TRP) channels are sensors for a wide range of cellular and environmental signals, but elucidating how these channels respond to physical and chemical stimuli has been hampered by a lack of detailed structural information ...
M. Liao, E. Cao, D. Julius, Yifan Cheng
semanticscholar   +1 more source

Molecular determinants of signal transduction in tropomyosin receptor kinases

FEBS Open Bio, EarlyView.
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley   +1 more source

Protocol for multi-scale light microscopy/electron microscopy neuronal imaging in mouse brain tissue. [PDF]

STAR Protoc, 2022
Yamauchi K, Furuta T, Okamoto S, Takahashi M, Koike M, Hioki H.   +5 more
europepmc   +1 more source

Segmentation of Sedimentary Grain in Electron Microscopy Image [PDF]

, 2013
This paper describes a novel method developed for the segmentation of sedimentary grains in electron microscopy images. The algorithm utilizes the approach of region splitting and merging.
Krizova, L., Krupka, A., Riha, K.
core   +1 more source

py4DSTEM: a software package for multimodal analysis of four-dimensional scanning transmission electron microscopy datasets [PDF]

, 2020
Scanning transmission electron microscopy (STEM) allows for imaging, diffraction, and spectroscopy of materials on length scales ranging from microns to atoms.
Anapolsky, Abraham, Barnard, Edward S, Brown, Hamish G, Ciston, Jim, DaCosta, Luis Rangel, Donohue, Jennifer, Ercius, Peter, Gopal, Chirranjeevi, Herring, Patrick, Hughes, Lauren A, Janish, Matthew T, Kennedy, Ellis, Minor, Andrew M, Ophus, Colin, Pekin, Thomas C, Pelz, Philipp M, Savitzky, Benjamin H, Schneider, Matthew M, Scott, Mary, Zeltmann, Steven E, Zhao, Shiteng   +20 more
core   +1 more source

The cryo-electron microscopy structure of huntingtin

Nature, 2018
Huntingtin (HTT) is a large (348 kDa) protein that is essential for embryonic development and is involved in diverse cellular activities such as vesicular transport, endocytosis, autophagy and the regulation of transcription.
Q. Guo, Bin Huang, Jingdong Cheng, Manuel Seefelder, Tatjana Engler, G. Pfeifer, P. Oeckl, M. Otto, Franziska Moser, M. Maurer, A. Pautsch, W. Baumeister, R. Fernández-Busnadiego, S. Kochanek   +13 more
semanticscholar   +1 more source

NOTCH2NLC Repeat Expansions in Parkinsonian Disorders: Clinical and Neuroimaging Characteristics

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Neuronal intranuclear inclusion disease (NIID) is a neurodegenerative disorder caused by NOTCH2NLC GGC repeat expansions, with heterogeneous clinical manifestations, including parkinsonism. Recent studies have identified NOTCH2NLC repeat expansions in patients with Parkinson's disease (PD) and atypical parkinsonism (aPM), suggesting ...
Han‐Lin Chiang, Kang‐Yang Jih, Cheng‐Tsung Hsiao, Fu‐Pang Chang, Justus Chunyu Chen, Yi‐Chu Liao, Yih‐Ru Wu, Yi‐Chung Lee   +7 more
wiley   +1 more source

Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaleSF. [PDF]

iScience, 2022
Furuta T, Yamauchi K, Okamoto S, Takahashi M, Kakuta S, Ishida Y, Takenaka A, Yoshida A, Uchiyama Y, Koike M, Isa K, Isa T, Hioki H.   +12 more
europepmc   +1 more source