Performance of LED-Based Fluorescence Microscopy to Diagnose Tuberculosis in a Peripheral Health Centre in Nairobi. [PDF]
, 2011 Sputum microscopy is the only tuberculosis (TB) diagnostic available at peripheral levels of care in resource limited countries. Its sensitivity is low, particularly in high HIV prevalence settings. Fluorescence microscopy (FM) can improve performance of
Bonnet, M +6 more
core +10 more sources
Fluorescence microscopy imaging with a Fresnel zone plate array based optofluidic microscope [PDF]
, 2011 We report the implementation of an on-chip microscope system, termed fluorescence optofluidic microscope (FOFM), which is capable of fluorescence microscopy imaging of samples in fluid media.
Han, Chao +3 more
core +1 more source
Scanless multitarget-matching multiphoton excitation fluorescence microscopy [PDF]
Journal of Innovative Optical Health Sciences, 2018 Using the combination of a reflective blazed grating and a reflective phase-only diffractive spatial light modulator (SLM), scanless multitarget-matching multiphoton excitation fluorescence microscopy (SMTM-MPM) was achieved.
Junpeng Qiu +4 more
doaj +1 more source
Light Sheet Fluorescence Microscopy Illuminating Soft Matter
Frontiers in Physics, 2021 Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
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Scan-less full-field fluorescence-lifetime dual-comb microscopy using two-dimensional spectral mapping and frequency multiplexing of dual-optical-comb beats [PDF]
Science Advances, Vol. 7, no. 1, eabd2102 (2021), 2020 Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust to photobleaching.
arxiv +1 more source
Instant three-color multiplane fluorescence microscopy
Biophysical Reports, 2021 One of the most widely used microscopy techniques in biology and medicine is fluorescence microscopy, offering high specificity in labeling as well as maximal sensitivity. For live-cell imaging, the ideal fluorescence microscope should offer high spatial
Ingo Gregor +3 more
doaj
Computational structured illumination for high-content fluorescent and phase microscopy [PDF]
, 2019 High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy.
Chowdhury, Shwetadwip +2 more
core +2 more sources
Frontiers in fluorescence microscopy
The International Journal of Developmental Biology, 2009 How we see organisms and cells depends on the tools at our disposal. For over 150 years, biologists were forced to rely on fixed, dehydrated and stained specimens in order to guess how the living cells could function. It all changed abruptly during the last two decades when the rapid development of novel imaging techniques revolutionized the way ...
Rino, José +3 more
openaire +4 more sources
Fluorescence Microscopy of the HIV-1 Envelope
Viruses, 2020 Human immunodeficiency virus (HIV) infection constitutes a major health and social issue worldwide. HIV infects cells by fusing its envelope with the target cell plasma membrane.
Pablo Carravilla +2 more
doaj +1 more source
Wide field fluorescence epi-microscopy behind a scattering medium enabled by speckle correlations [PDF]
Opt. Express 26, 9866-9881 (2018), 2018 Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and exploit the optical memory effect to enable fluorescence microscopy through a turbid layer.
arxiv +1 more source