Results 51 to 60 of about 2,648,352 (332)
ixFLIM: Interferometric Excitation Fluorescence Lifetime Imaging Microscopy [PDF]
Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it provides information about the emitting state only. Here, we present an extension of FLIM by interferometric measurement of fluorescence excitation spectra.
arxiv
Integral refractive index imaging of flowing cell nuclei using quantitative phase microscopy combined with fluorescence microscopy [PDF]
We suggest a new multimodal imaging technique for quantitatively measuring the integral (thickness-average) refractive index of the nuclei of live biological cells in suspension. For this aim, we combined quantitative phase microscopy with simultaneous 2-D fluorescence microscopy.
arxiv +1 more source
Correlated light and electron microscopy (CLEM) has become a popular technique for combining the protein-specific labeling of fluorescence with electron microscopy, both at room and cryogenic temperatures.
L. Metskas, John A. G. Briggs
semanticscholar +1 more source
Calcium‐sensing receptor‐ and ADAM10‐mediated klotho shedding is regulated by tetraspanin 5
The G protein‐coupled calcium‐sensing receptor (CaSR) and the disintegrin/metalloprotease ADAM10 in the renal distal convoluted tubule control systemic levels of Klotho, a cell and tissue homeostasis‐preserving factor. We show that CaSR‐induced cleavage of membrane‐bound αKlotho by ADAM10 requires tetraspanin 5 (Tspan5), a scaffolding and chaperone ...
Zhenan Liu+4 more
wiley +1 more source
Hermansky‐Pudlak syndrome type 1 (HPS‐1) is a rare, autosomal recessive disorder with poorly understood renal involvement. Urinary extracellular vesicle (uEV) proteomics and a novel Hps1 mouse model reveal mitochondrial abnormalities and lipid accumulation in HPS‐1 kidney proximal tubule cells. Serum ApoA1 correlates with kidney function in our patient
Dawn M. Maynard+7 more
wiley +1 more source
Three-dimensional bi-functional refractive index and fluorescence microscopy (BRIEF) [PDF]
Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical property that quantitatively relates to cell morphology, mass, and stiffness.
arxiv
Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo ...
Jianglai Wu+9 more
semanticscholar +1 more source
The human Na+/H+ antiporter NHA2 transports Na+ or Li+ in exchange for protons across cell membranes, and its dysfunction results in various pathologies, e.g., fertility disorders, hypertension, or diabetes. This work provides the molecular characterization of interactions of NHA2 with its specific inhibitor phloretin, which can be helpful for the ...
Olga Zimmermannova+3 more
wiley +1 more source
miR-203 drives breast cancer cell differentiation
A hallmark of many malignant tumors is dedifferentiated (immature) cells bearing slight or no resemblance to the normal cells from which the cancer originated.
Nuria G. Martínez-Illescas+20 more
doaj +1 more source
Polarization effects on fluorescence emission of zebrafish neurons using light-sheet microscopy [PDF]
Light-sheet fluorescence microscopy (LSFM) makes use of a thin plane of light to optically section and image transparent tissues or organisms {\it{in vivo}}, which has the advantages of fast imaging speed and low phototoxicity. In this paper, we have employed light-sheet microscopy to investigate the polarization effects on fluorescence emission of ...
arxiv