Results 101 to 110 of about 8,924,569 (266)

The use of SMALPs as a novel membrane protein scaffold for structure study by negative stain electron microscopy. [PDF]

, 2015
Despite the great progress recently made in resolving their structures, investigation of the structural biology of membrane proteins still presents major challenges.
Shaun Rawson, Mitchell, JK, Postis, Vincent; id_orcid, Vincent Postis, Tim R Dafforn, Postis, Vincent, Muench, Stephen P., Baldwin, SA, Rawson, Shaun, Baldwin, Stephen A., Postis, V, Rawson, S, Stephen A Baldwin, Lee, SC, Rosemary A Parslow, Mitchell, Jennifer K., Dafforn, TR, Lee, Sarah C., Parslow, Rosemary A., Jennifer K Mitchell, Sarah C Lee, Stephen P Muench, Muench, SP, Dafforn, Tim R., Parslow, RA   +24 more
core   +1 more source

Exploring the influence of lactoferrin on wound healing and related signaling pathways: a systematic review

FEBS Open Bio, EarlyView.
Lactoferrin promotes wound healing by activating MAPK, PI3K/Akt, and extracellular matrix pathways, enhancing fibroblast remodeling, proliferation, and re‐epithelialization. These mechanisms highlight its therapeutic potential in inflammation control, tissue repair, and remodeling.
Morgana Lüdtke Azevedo, Kátia Cristiane Hall, Matheus dos Santos Fernandez, Geovanna Peter Corrêa, Rafael Guerra Lund   +4 more
wiley   +1 more source

Detection of Cryptosporidium - specific coproantigen in human immunodeficiency virus/acquired immunodeficiency syndrome patients by using a commercially available immunoenzymatic assay

Memorias do Instituto Oswaldo Cruz, 2003
The aim of this study was to verify the occurrence of Cryptosporidium infection in 52 human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients (group 1) and 38 clinically healthy individuals (group 2) by using enzyme ...
Claudio Vieira Silva, Marcelo Simão Ferreira, Maria do Rosário de Fátima Gonçalves-Pires, Julia Maria Costa-Cruz   +3 more
doaj   +1 more source

KDAC6 alters cell morphology and motility through positive and negative modulation of F‐actin distribution

FEBS Open Bio, EarlyView.
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph, Andrea B. Pham, Kiara E. Bornes, Razan H. Hammad, Chelsea A. Rousseve‐Ross, Thomas M. Huckaba, Terry J. Watt, Tasha B. Toro   +7 more
wiley   +1 more source

Enzyme-linked immunosorbent assay (ELISA) immunoassaying versus microscopy: advantages and drawbacks for diagnosing giardiasis

São Paulo Medical Journal
CONTEXT AND OBJECTIVE: Giardiasis is common in Brazil. For laboratory diagnosis, the method most utilized is microscopic examination of fecal samples, but the immunoenzymatic method is also available. The aim of this work was to verify the advantages and
Alaíde Mader Braga Vidal, Wilson Roberto Catapani   +1 more
doaj   +1 more source

Evaluation of enzyme linked immunosorbent assay for stool antigen detection for the diagnosis of cryptosporidiosis among HIV negative immunocompromised patients in a tertiary care hospital of northern India

Journal of Infection and Public Health, 2018
The diagnosis of cryptosporidiosis among HIV positive patients has been the focus of many studies worldwide. However, there is a paucity of data on HIV negative immunocompromised patients like post-renal transplant recipients and those with ...
Ujjala Ghoshal, Vidhi Jain, Asmita Dey, Prabhat Ranjan   +3 more
doaj   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Invasive Species Monitoring Is Improved by Combining eDNA qPCR and Traditional Microscopy Methods

Aquaculture, Fish and Fisheries
Preventing the spread of aquatic invasive species, such as non‐native molluscs, plants, and fish, hinges on early detection to help managers avoid establishment. Here, we evaluated two detection methods for a prolific invader: dreissenid mussels.
Zoe Dahlquist, Dulaney L. Miller, Stephen J. Amish, Leif Howard, Michael McCartney, Gordon Luikart   +5 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source