Results 81 to 90 of about 8,924,569 (266)

Implementation of a National Reference Laboratory for Buruli Ulcer Disease in Togo [PDF]

open access: yes, 2013
Background: In a previous study PCR analysis of clinical samples from suspected cases of Buruli ulcer disease (BUD) from Togo and external quality assurance (EQA) for local microscopy were conducted at an external reference laboratory in Germany.
Vogel Felix   +83 more
core   +2 more sources

Evaluation of Multiplex loop-mediated isothermal amplification assay for the detection of Mycobacterium tuberculosis complex from clinically suspected cases of pulmonary tuberculosis

open access: yesHeliyon
Tuberculosis (TB) is the second leading cause of death from a single infectious agent worldwide. Bangladesh ranks 7th among the 30 high TB burdened countries in the world. Accurate detection of Mycobacterium tuberculosis complex (MTBC) is challenging for
Md Zaber   +3 more
doaj   +1 more source

Molecular and microscopic detection of natural and experimental infections of Toxocara vitulorum in bovine milk.

open access: yesPLoS ONE, 2020
Toxocara vitulorum is an Ascarid nematode infecting the small intestine of buffalo and cattle particularly neonate calves, with the postnatal route through milk is the main infection source.
Amira Dewair, Mohamed Bessat
doaj   +1 more source

Overcoming platinum resistance by the disulfiram metabolite CuET in novel preclinical models of human carcinomas

open access: yesMolecular Oncology, EarlyView.
Cisplatin‐resistant cancer cells survive DNA‐damaging platinum‐based treatment yet remain sensitive to CuET‐induced proteotoxic stress. The distinct mechanism of action allows CuET to overcome and eliminate cisplatin‐resistant tumour cells, unless highly expressed metallothioneins (MTs) interfere with CuET, thereby highlighting MT levels as candidate ...
Stefanos Arampatzis   +20 more
wiley   +1 more source

Electron microscopy of Pepper mild mottle virus (PMMoV)-RNA positive samples.

open access: yes, 2013
Footnote: The samples analyzed by electron microscopy were (a) Tabasco sauce; (b) PMMoV purified from Tabasco sauce. The samples analyzed following immunogold staining were: (c) PMMoV purified from Tabasco sauce; (d) a PMMoV-RNA positive patient's stool ...
Christelle Desnues (256122)   +10 more
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

open access: yesFEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová   +2 more
wiley   +1 more source

Diagnosis of Plasmodium infections using artificial intelligence techniques versus standard microscopy in a reference laboratory

open access: yesJournal of Clinical Microbiology
Diagnosing malaria using standard techniques is time-consuming. With limited staffing in many laboratories, this may lead to delays in reporting. Innovative technologies are changing the diagnostic landscape and may help alleviate staffing shortages. The
Sanjai Nagendra   +3 more
doaj   +1 more source

O-5S quantitative real-time PCR: a new diagnostic tool for laboratory confirmation of human onchocerciasis

open access: yesParasites & Vectors, 2017
Background Onchocerciasis is a parasitic disease caused by the filarial nematode Onchocerca volvulus. In endemic areas, the diagnosis is commonly confirmed by microscopic examination of skin snip samples, though this technique is considered to have low ...
Solomon A. Mekonnen   +12 more
doaj   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

open access: yesFEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

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