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Current Opinion in Cell Biology, 2003
Microtubule nucleation is the process in which several tubulin molecules interact to form a microtubule seed. Microtubule nucleation occurs spontaneously in purified tubulin solutions, and molecular intermediates between tubulin dimers and microtubules have been identified.
Didier, Job +2 more
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Microtubule nucleation is the process in which several tubulin molecules interact to form a microtubule seed. Microtubule nucleation occurs spontaneously in purified tubulin solutions, and molecular intermediates between tubulin dimers and microtubules have been identified.
Didier, Job +2 more
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Centrosome-microtubule nucleation
Journal of Cell Science, 1997ABSTRACT In many cell types the formation of microtubules from tubulin subunits is initiated at defined nucleation sites at the centrosome. Thesse sites contain the conserved γ-tubulin which is in association with additional not very well characterised proteins, identified as components of a γ-tubulin ring complex from Xenopus egg ...
Pereira, G, Schiebel, E
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Spontaneous nucleation of microtubules
Physical Review E, 1995The spontaneous nucleation of individual microtubules from tubulin dimers is observed directly under conditions of marginal nucleation. The results are compared with measurements of bulk turbidity following a deep quench [W. A. Voter and H. P. Erickson, J. Biol. Chem. 259, 10 430 (1984)].
, Kuchnir Fygenson D +4 more
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Microtubule-dependent microtubule nucleation in plant cells
Journal of Plant Research, 2006Regulation of microtubule nucleation sites is an essential step in microtubule organization. Cortical microtubule arrays in green plant cells at inter-phase are organized in a distinct manner--the array is formed in the absence of previously recognized organelles for microtubule nucleation, for example the centrosome and spindle pole body. Microtubules
Takashi, Murata, Mitsuyasu, Hasebe
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Microtubule nucleation: beyond the template
Nature Reviews Molecular Cell Biology, 2017Microtubules are cytoskeletal filaments central to a wide range of essential cellular functions in eukaryotic cells. Consequently, cells need to exert tight control over when, where and how many microtubules are being made. Whereas the regulation of microtubule dynamics is well studied, the molecular mechanisms of microtubule nucleation are still ...
Johanna Roostalu, Thomas Surrey
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Assaying Microtubule Nucleation
2019Assaying microtubule nucleation is essential to understand the organization of microtubule networks in any cell type. In this chapter we describe methods for measuring nucleation activity at centrosomes and at mitotic chromatin in cell lines, to study interphase and mitotic microtubule organization, and for measuring non-centrosomal nucleation in ...
Artur, Ezquerra +2 more
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Microtubule-associated proteins control the kinetics of microtubule nucleation
Nature Cell Biology, 2015Microtubules are born and reborn continuously, even during quiescence. These polymers are nucleated from templates, namely γ-tubulin ring complexes (γ-TuRCs) and severed microtubule ends. Using single-molecule biophysics, we show that nucleation from γ-TuRCs, axonemes and seed microtubules requires tubulin concentrations that lie well above the ...
Michal, Wieczorek +3 more
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Cell biology: Kinetochores nucleate their own microtubules
Current Biology, 2023The origin of microtubules that tether the chromosomes' kinetochores to spindle poles has remained a mystery for several decades. A new study identifies the 'fibrous corona' as an autonomous microtubule nucleation site, and reveals the molecular cascade responsible for this process.
Fioranna, Renda, Alexey, Khodjakov
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γ-Tubulin complexes and microtubule nucleation
Current Opinion in Structural Biology, 2001Microtubules are dynamic cytoskeletal polymers that assemble from alpha/beta-tubulin and are vital for the establishment of cell polarity, vesicle trafficking and formation of the mitotic/meiotic spindle. gamma-Tubulin, a protein related to alpha/beta-tubulin, is required for initiating the polymerization of microtubules in vivo. gamma-Tubulin has been
M, Moritz, D A, Agard
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Microtubule assembly nucleated by isolated centrosomes
Nature, 1984Microtubules are involved in the morphogenesis of most cells and are the structural basis of the mitotic spindle. We report here that purified centrosomes nucleate the assembly of microtubules with unusual dynamic properties. This may have important implications for the mechanism by which microtubule arrays are organized and stabilized in cells.
T, Mitchison, M, Kirschner
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