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Isolation and Culture of Microvascular Endothelial Cells
2003The cultivation of endothelial cells from large vessels, predominantly from human umbilical veins (1,2), has become a routine procedure in many laboratories and has contributed to the development of modern vascular biology. However, there is convincing evidence that microvascular endothelial cells display a number of important functional differences ...
L, Richard, P, Velasco, M, Detmar
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Homocysteine in Microvascular Endothelial Cell Barrier Permeability
Cell Biochemistry and Biophysics, 2005Redox stress activates the endothelium and upregulates matrix metalloproteinases (MMPs), which degrade the matrix and lead to blood-endothelial barrier leakage. Interestingly, elevated levels of plasma homocysteine (Hcy) are associated with vascular dementia, seizure, stroke, and Alzheimer disease. Hcy competes with the gamma-aminobutyric acid (GABA)-A/
Suresh C, Tyagi +2 more
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Microvascular endothelial cell heterogeneity
Cell and Tissue Research, 2009Microvascular endothelial cells display a large degree of heterogeneity in function depending on their location in the vascular tree. The existence of organ-specific, microvascular-bed-specific, and even intravascular variations in endothelial cell gene expression emphasizes their high cell-to-cell variability, which is furthermore extremely adaptable ...
Langenkamp, Elise, Molema, Grietje
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Metabolism of anandamide in cerebral microvascular endothelial cells
Prostaglandins & Other Lipid Mediators, 2004Anandamide (N-arachidonoylethanolamine, AEA), an endogenous cannabinoid receptor agonist, causes potent vasodilation in the cerebral circulation through an endothelial-dependent or -independent mechanism. We have investigated the processing of [3H]AEA in cultured mouse cerebral microvascular endothelial cells (MEC) in order to better understand its ...
Ping, Chen +5 more
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Proproliferative phenotype of pulmonary microvascular endothelial cells
American Journal of Physiology-Lung Cellular and Molecular Physiology, 2007Endothelial cells perform a number of important functions including release of vasodilators, control of the coagulation cascade, and restriction of solutes and fluid from the extravascular space. Regulation of fluid balance is of particular importance in the microcirculation of the lung where the loss of endothelial barrier function can lead to ...
Victor, Solodushko, Brian, Fouty
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ISOLATION AND CULTURE OF RAT MICROVASCULAR ENDOTHELIAL CELLS
In Vitro Cellular & Developmental Biology - Animal, 2002The purpose of this study is to identify the separation techniques that result in pure cultures of rat microvascular endothelial cells (MECs). A multistep process is used to optimize the separation of the cells from rat epididymal fat pads, obtaining as pure a culture as possible within a relatively short processing time.
Cynthia A, Frye, Charles W, Patrick
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Culture and characterization of pulmonary microvascular endothelial cells
In Vitro Cellular & Developmental Biology - Animal, 1992Surface proteins were compared in endothelial cells (EC) obtained from bovine peripheral lung, pulmonary artery and vein, and dorsal aorta using sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. Galactose-containing glycoproteins [molecular weight (M(r)) 160-220 and 40 kDa] binding to the Ricinus communis agglutinin (RCA) and peanut ...
P J, Del Vecchio +5 more
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Vitronectin decreases microvascular endothelial cell apoptosis
Journal of Cellular Physiology, 1998Angiogenesis after tissue injury occurs in a matrix environment consisting of fibrin, fibronectin, and vitronectin as the major extracellular matrix (ECM) constituents. ECM-integrin interactions is critical for angiogenesis and failure to bind a ligand to certain integrin receptors (alpha[v]beta3 or alpha[v]beta5) inhibits angiogenesis. The ligand that
F F, Isik +4 more
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Journal of Cellular Physiology, 1992
AbstractMicromolar concentrations of sodium orthovanadate stimulated the proliferation of bovine capillary endothelial cells, but not bovine aortic endothelial cells. Vanadate was equally potent at inducing protein tyrosine phosphorylation and changes in morphology in both types of cells.
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AbstractMicromolar concentrations of sodium orthovanadate stimulated the proliferation of bovine capillary endothelial cells, but not bovine aortic endothelial cells. Vanadate was equally potent at inducing protein tyrosine phosphorylation and changes in morphology in both types of cells.
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The expression of ADAMTS13 in human microvascular endothelial cells
Blood Coagulation & Fibrinolysis, 2016ADAMTS13, as a specific von Willebrand factor (VWF)-cleaving protease, prevents microvascular thrombosis of VWF/platelet thrombi. It has been reported that human vascular endothelial cells could also synthesize and secrete ADAMTS13, and these reports were focused in human umbilical vascular endothelial cells.
Anyou, Wang +4 more
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