Results 271 to 280 of about 204,813 (306)

Isolation and Culture of Microvascular Endothelial Cells

2003
The cultivation of endothelial cells from large vessels, predominantly from human umbilical veins (1,2), has become a routine procedure in many laboratories and has contributed to the development of modern vascular biology. However, there is convincing evidence that microvascular endothelial cells display a number of important functional differences ...
L, Richard, P, Velasco, M, Detmar
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VEGF differentially activates STAT3 in microvascular endothelial cells

The FASEB Journal, 2003
ABSTRACT Increased VEGF expression is found in several pathologies characterized by abnormal angiogenesis. Previous studies have shown that the transcription factor STAT3 mediates VEGF gene transcription and its activation. In this study, Western analysis and confocal immunocytochemistry were used to examine STAT3 activation in ...
Manuela, Bartoli, Dan, Platt, Tahira, Lemtalsi, Xiaolin, Gu, Steven E, Brooks, Mario B, Marrero, Ruth B, Caldwell   +6 more
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Dexamethasone effects on microvascular endothelial cell lipid composition

American Journal of Physiology-Cell Physiology, 1989
Previous studies have shown that treatment of cultured rabbit coronary microvessel endothelial (RCME) cells with dexamethasone results in a dose-, time-, and glucocorticoid-dependent inhibition of prostaglandin release. In the present study, the effects of dexamethasone on RCME membrane lipid composition and release of arachidonic acid were examined ...
M S, Medow, L, Intrieri, T, Moatter, M E, Gerritsen   +3 more
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Uptake of suramin by human microvascular endothelial cells

Cancer Letters, 1998
We have demonstrated for the first time that suramin is taken up by human dermal microvascular endothelial (HMEC-1) cells by an active process involving the caveolae system. The uptake of suramin was time-dependent and reduced by more than 90% when incubated in the presence of albumin or at 4 degrees C.
A R, Gagliardi, M F, Taylor, D C, Collins   +2 more
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Metabolism of anandamide in cerebral microvascular endothelial cells

Prostaglandins & Other Lipid Mediators, 2004
Anandamide (N-arachidonoylethanolamine, AEA), an endogenous cannabinoid receptor agonist, causes potent vasodilation in the cerebral circulation through an endothelial-dependent or -independent mechanism. We have investigated the processing of [3H]AEA in cultured mouse cerebral microvascular endothelial cells (MEC) in order to better understand its ...
Ping, Chen, Shanming, Hu, Shawn D, Harmon, Steven A, Moore, Arthur A, Spector, Xiang, Fang   +5 more
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Bovine microvascular endothelial cells immortalized with human telomerase

Journal of Cellular Biochemistry, 2006
AbstractPrimary cultures of bovine microvascular endothelial cells (BME) isolated from the adrenal cortex, are commonly used to study vascular endothelium, but have a limited life span. To circumvent these limitations, we have immortalized BME cells with either simian virus 40 (SV40) or with a retrovirus containing the coding region of human telomerase
Buser Llinares, Raphaële, Montesano, Roberto, Garcia, Irène, Dupraz, Philippe, Pepper, Michael Sean   +4 more
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FVIII production by human lung microvascular endothelial cells

Blood, 2006
While extrahepatic factor VIII (FVIII) synthesis suffices for hemostasis, the extrahepatic production sites are not well defined. We therefore investigated the ability of the human lungs to produce FVIII. Lungs from heart-beating donors who were declined for transplantation were perfused and ventilated in an isolated reperfusion model for 2 hours.
Marc, Jacquemin, Arne, Neyrinck, Maria Iris, Hermanns, Renaud, Lavend'homme, Filip, Rega, Jean-Marie, Saint-Remy, Kathelijne, Peerlinck, Dirk, Van Raemdonck, Charles James, Kirkpatrick   +8 more
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Erythromycin-induced CXCR4 expression on microvascular endothelial cells

American Journal of Physiology-Lung Cellular and Molecular Physiology, 2009
Although stromal-derived factor-1 (SDF-1) via its cognate receptor CXCR4 is assumed to play a critical role in migration of endothelial cells during new vessel formation after tissue injury, CXCR4 expression on endothelial cells is strictly regulated. Erythromycin (EM), a 14-membered ring macrolide, has an anti-inflammatory effect that may account for ...
Yasuyuki, Takagi, Naozumi, Hashimoto, Sem H, Phan, Kazuyoshi, Imaizumi, Masaki, Matsuo, Harunori, Nakashima, Izumi, Hashimoto, Yuta, Hayashi, Tsutomu, Kawabe, Kaoru, Shimokata, Yoshinori, Hasegawa   +10 more
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ISOLATION AND CULTURE OF RAT MICROVASCULAR ENDOTHELIAL CELLS

In Vitro Cellular & Developmental Biology - Animal, 2002
The purpose of this study is to identify the separation techniques that result in pure cultures of rat microvascular endothelial cells (MECs). A multistep process is used to optimize the separation of the cells from rat epididymal fat pads, obtaining as pure a culture as possible within a relatively short processing time.
Cynthia A, Frye, Charles W, Patrick
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Microvascular Endothelial Cell Shape and Size in Situ

Microvascular Research, 1993
To estimate total cleft length per unit surface area, previous studies assumed that endothelial cell clefts were oriented randomly with respect to the axis of the microvessel (Bundgaard and Frøkjaer-Jensen, Microvasc. Res. 23, 1-30, 1982). In the present study, silver precipitation along the intercellular clefts of capillary endothelium ("silver lines")
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