Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Migration as an Adaptation Strategy and Its Consequences on Coastal Society: Experience from Bangladesh [PDF]
Mallick B. Migration as an Adaptation Strategy and Its Consequences on Coastal Society: Experience from Bangladesh. COMCAD Arbeitspapiere - Working Papers, 96.
Mallick, Bishawjit
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Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
The migration-remittances-development-nexus: ‘Mi casa con Remesas’ and transnational flows between Colombia and London [PDF]
Zapata GP. The migration-remittances-development-nexus: ‘Mi casa con Remesas’ and transnational flows between Colombia and London. COMCAD Arbeitspapiere - Working Papers, 106.
Zapata, Gisela P.
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Directed evolution of enzymes at the crossroads of tradition and innovation
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Environmental Change and Migration: Governmental Compensation Policies to Natural Disasters Victims and Urbanization Process: A Case Study of Wildfires in Russian Federation in 2010 [PDF]
Bobylev N. Environmental Change and Migration: Governmental Compensation Policies to Natural Disasters Victims and Urbanization Process: A Case Study of Wildfires in Russian Federation in 2010. COMCAD Arbeitspapiere - Working Papers, 100.
Bobylev, Nikolai
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ABSTRACT Background Ischemic stroke, a major cause of mortality and long‐term disability, results from the abrupt cessation of cerebral blood flow due to vascular occlusion or rupture. Icosapent Ethyl (EPA‐EE), approved for hypertriglyceridemia, has anti‐inflammatory and antithrombotic properties that may lessen ischemic damage.
Mitra Mahmoudi Meymand +5 more
wiley +1 more source
Care migration from rural Hungary [PDF]
szabadon elérhető / Free accessInternational Migration Conference: EU at the crossroads of migration: critical reflections on the ‘refugee crisis’ and new migration deals. Utrecht, Hollandia, 2018.
Váradi, Monika Mária +1 more
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Predictive Value of Composite Inflammatory Markers for Stroke Prognosis: A Prospective Cohort Study
ABSTRACT Background Novel composite inflammatory markers' role in stroke prognosis is understudied, and the best predictor is unclear, requiring further exploration. Objectives This study aimed to systematically evaluate the associations of 6 novel composite inflammatory markers on stroke prognosis.
Bing Wu +7 more
wiley +1 more source
Summary report on the MIPEX health strand and country reports [PDF]
The Migrant Integration Policy Index (MIPEX) Health strand is a questionnaire designed to supplement the existing seven strands of the MIPEX, which in its latest edition (2015) monitors policies affecting migrant integration in 38 different countries ...
International Organization for Migration +1 more
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