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Minigene as a Novel Regulatory Element in Toxin-Antitoxin Systems [PDF]

International Journal of Molecular Sciences, 2021
The axe-txe type II toxin-antitoxin (TA) system is characterized by a complex and multilayered mode of gene expression regulation. Precise and tight control of this process is crucial to keep the toxin in an appropriate balance with the cognate antitoxin until its activation is needed for the cell.
Katarzyna Potrykus
exaly   +3 more sources

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The multivalent minigene approach to vaccine development

Expert Opinion on Investigational Drugs, 1999
Epitope based minigenes (epigenes) have been under investigation for several years as an experimental approach to vaccination against infectious diseases. The essence of this technology is that short DNA sequences, encoding well-defined cytotoxic T-lymphocyte- (CTL), antibody- (Ab) or helper T-lymphocyte- (HTL) specific epitopes are used as immunogens.
Alessandro Sette
exaly   +3 more sources

Distribution of minigenes in the bacteriophage lambda chromosome

Gene, 2004
The bar loci in the chromosome of bacteriophage lambda inhibit phage vegetative growth in bacteria defective for peptidyl-tRNA hydrolase (Pth). Expression of the bar regions results in accumulation of peptidyl-tRNA, inhibition of protein synthesis, and arrest of mutant cell growth.
Norma-Angélica, Oviedo, Heladia, Salgado, Julio, Collado-Vides, Gabriel, Guarneros   +3 more
openaire   +2 more sources

An Amelogenin Minigene to Study Alternative Splicing

DNA and Cell Biology, 2006
Diversity in gene expression is commonly observed as a result of alternative splicing of RNA transcripts. This is true in the case of amelogenin, one of the enamel matrix proteins. Our hypothesis is that additional amelogenin mRNA transcripts are generated in vivo, but these transcripts have yet to be observed because of the limitations of currently ...
Jason L, Shapiro, Hongjun, Wang, Xin, Wen, Sissada, Tannukit, Michael L, Paine   +4 more
openaire   +2 more sources

Regulation of protein synthesis by minigene expression

Biochimie, 1997
Peptidyl-tRNA hydrolase (Pth), an enzyme essential for Escherichia coli viability, scavenges peptidyl-tRNA released during abortive polypeptide chain elongation. Bacterial strains of E coli partially defective in Pth activity are unable to maintain bacteriophage lambda growth.
J, Hernández, C, Ontiveros, J G, Valadez, R H, Buckingham, G, Guarneros   +4 more
openaire   +2 more sources

Cell-Based Splicing of Minigenes

2014
Cell-based splicing of minigenes is used extensively in the analysis of alternative splicing events. In particular, such assays are critical for identifying or confirming the in vivo relevance of cis- and trans-acting factors in the regulation of particular splicing patterns.
Sarah A, Smith, Kristen W, Lynch
openaire   +2 more sources

Minigenes to Confirm Exon Skipping Mutations

2012
Although several bioinformatic tools exist to predict the effect on splicing of a nucleotide change, experimental verification with minigenes is essential for diagnostic purposes, as well as for revealing disease mechanisms and monitoring therapeutic interventions.
Desviat, Lourdes R., Pérez, Belén, Ugarte, Magdalena   +2 more
openaire   +3 more sources

Protection by minigenes: a novel approach of DNA vaccines

Vaccine, 1998
To test the principle that genetically engineered epitopes in a plasmid DNA can efficiently induce specific immunity, a minigene cassette encoding cytotoxic T lymphocyte (CTL), helper T and B cell epitopes from herpes simplex virus (HSV) was constructed and placed in an expression vector named pcMini. Following immunizations with pcMini, mice developed
Z, Yu, K L, Karem, S, Kanangat, E, Manickan, B T, Rouse   +4 more
openaire   +2 more sources

DNA Minigene Vaccination for Adjuvant Neuroblastoma Therapy

Annals of the New York Academy of Sciences, 2004
Abstract: The disruption of self‐tolerance against neuroblastoma is the ultimate goal of an effective DNA‐vaccine. We demonstrate the induction of protective immunity against syngeneic murine NXS2 neuroblastoma in A/J mice following vaccination with tyrosine hydroxylase (TH)‐derived antigens.
Holger N, Lode, Nicole, Huebener, Yan, Zeng, Stefan, Fest, S, Weixler, Gerhard, Gaedicke   +5 more
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Human Antithrombin III Minigene with an Optimized Splicing Pattern

Molecular Biology, 2019
Antithrombin III (AT3) belongs to the superfamily of serine protease inhibitors (serpins) and is a major anticoagulant in physiological conditions. Based on SERPINC1 gene, a minigene coding for human AT3, which is valuable for medicine and biotechnology, was constructed by minimizing the size of lengthy introns and preserving the splicing site-flanking
M V, Shepelev, E K, Saakian, S V, Kalinichenko, I V, Korobko   +3 more
openaire   +2 more sources