Results 141 to 150 of about 9,190 (179)

The in vivo minigene approach to analyze tissue-specific splicing

Brain Research Protocols, 1999
The exact mechanisms leading to alternative splice site selection are still poorly understood. However, recently cotransfection studies in eukaryotic cells were successfully used to decipher contributions of RNA elements (cis-factors), their interacting protein components (trans-factors) or the cell type to alternative pre-mRNA splicing. Splice factors
O, Stoss, P, Stoilov, A M, Hartmann, O, Nayler, S, Stamm   +4 more
openaire   +2 more sources

Minigene rescues acetylcholinesterase lethal mutations in Drosophila melanogaster

Journal of Molecular Biology, 1992
The gene encoding acetylcholinesterase in Drosophila melanogaster is over 34,000 base-pairs long. We have constructed a 5800 base-pair minigene containing 1500 base-pairs of genomic sequence upstream from the transcription start spliced to the coding sequence, but lacking the nine introns.
Hoffmann, F., Fournier, Dominique, Spierer, P.   +2 more
openaire   +5 more sources

Use of minigene systems to dissect alternative splicing elements

Methods, 2005
Pre-mRNA splicing is an essential step for gene expression in higher eukaryotes. The splicing efficiency of individual exons is determined by multiple features involving gene architecture, a variety of cis-acting elements within the exons and flanking introns, and interactions with components of the basal splicing machinery (called the spliceosome) and
openaire   +2 more sources

Gene Transfer of a Murine Dystrophin Minigene Construct

1992
Successful gene correction of dystrophin-deficient muscle cells depends on both the availability of a functional dystrophin cDNA construct and an understanding of the conditions required for efficient gene transfer and subsequent stable expression.
P. R. Clemens, C. C. Lee, C. T. Caskey
openaire   +1 more source

Functional Evaluation of Splice Variants Using a Minigene Strategy

Suspected splicing variants can be very challenging to evaluate more particularly when RNA samples are not available to perform RNA sequencing or when the gene of interest is expressed in specific tissues not easily reachable. One alternative strategy to study the potential splicing effect of a variant is to use the patient's DNA.
Angélique Nizou, Lana Mahfoud, Alexandre Janin, Anne-Sophie Lia   +3 more
openaire   +2 more sources

Hybrid Minigene Assay: An Efficient Tool to Characterize mRNA Splicing Profiles of NF1 Variants

Cancers, 2021
Leonardo Salviati, Maurizio Clementi, Eva Trevisson   +2 more
exaly  

minigene, n.

2023
openaire   +1 more source

Principles and Practical Considerations for the Analysis of Disease-Associated Alternative Splicing Events Using the Gateway Cloning-Based Minigene Vectors pDESTsplice and pSpliceExpress

International Journal of Molecular Sciences, 2021
Michael Hecker, Peter A Lorenz, Uwe Zettl   +2 more
exaly  

Idiotypic Determinants, Minigenes, and the Antibody Combining Site

1984
Idiotypic determinants, idiotopes, are best defined as antigenic determinants located on the Fv fragment of an antibody molecule. Since the demonstration that myeloma antibodies from different individuals showed characteristic distinct antigenic specificities(1,2) and the subsequent findings that such antigenic determinants were present on induced ...
openaire   +1 more source

Minigene-Based Splice Assays Reveal the Effect of Non-Canonical Splice Site Variants in USH2A

International Journal of Molecular Sciences, 2022
Marco Aben, Susanne Roosing, Hannie Kremer   +2 more
exaly