Results 171 to 180 of about 4,208 (187)

High-throughput multiplex MIRU-VNTR typing of Mycobacterium bovis

Research in Veterinary Science, 2014
Spoligotyping is the most widely used method for genotyping Mycobacterium bovis (M. bovis). However, its discriminatory power varies widely between countries. MIRU-VNTR typing could be a promising alternative, although it generally requires the time consuming and laborious simplex PCR assays using standard agarose gel electrophoresis.
Y, Navarro   +6 more
openaire   +2 more sources

Different Mycobacterium avium subsp. paratuberculosis MIRU–VNTR patterns coexist within cattle herds

Veterinary Microbiology, 2011
A better understanding of the biodiversity of Mycobacterium avium subsp. paratuberculosis (MAP) offers more insight in the epidemiology of paratuberculosis and therefore may contribute to the control of the disease. The aim of this study was to investigate the genetic diversity in bovine MAP isolates using PCR-based methods detecting genetic elements ...
van Hulzen, K.J.E.   +5 more
openaire   +4 more sources

Evaluation of Spoligotyping and MIRU-VNTR for Mycobacterium bovis in Xinjiang, China

Research in Veterinary Science, 2012
Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB) in cattle, is also a pathogen for human and other mammals. In this study, 406 cows were screened for bTB by both single intradermal comparative cervical tuberculin (SICCT) test and IFN-γ assay. 135 M.
Zhaogang, Sun   +9 more
openaire   +2 more sources

MIRU-VNTR analysis of the Mycobacterium tuberculosis isolates from three provinces of Iran

Scandinavian Journal of Infectious Diseases, 2012
Iran borders 2 high-burden tuberculosis (TB) countries to the east, and has the highest rates of TB in one of its eastern provinces. Limited information is available on the genetic diversity and transmission dynamics of Mycobacterium tuberculosis (MTB) in Iran.
Samin, Zamani   +7 more
openaire   +2 more sources

Evaluation of 24 locus MIRU-VNTR genotyping of Mycobacterium tuberculosis isolates in Canada

Tuberculosis, 2010
The current gold standard for Mycobacterium tuberculosis complex (MTBC) genotyping is insertion sequence (IS) 6110 restriction fragment length polymorphism (RFLP) as it provides the highest discriminatory power of all available MTBC genotyping methods. However, RFLP is labour intensive and the interpretation of data from this method can be susceptible ...
Sara, Christianson   +8 more
openaire   +2 more sources

Molecular genotyping of Mycobacterium tuberculosis in Xi'an, China, using MIRU-VNTR typing

The International Journal of Tuberculosis and Lung Disease, 2011
The molecular genotyping of Mycobacterium tuberculosis is expected to lead to a better understanding of M. tuberculosis transmission in Xi'an, one of the largest cities in Western China.To characterize the population of M. tuberculosis strains circulating in Xi'an and to evaluate the feasibility of the mycobacterial interspersed repetitive units ...
A, Zhou   +5 more
openaire   +2 more sources

Comparison of MIRU-VNTR genotyping between old and fresh clinical samples in tuberculosis

Infectious Diseases, 2019
Background: Twenty-four loci mycobacterial interspersed repetitive unit-variable number tandem repeat analysis (MIRU-VNTR) is extensively used for genotyping and detection of polyclonal infections in tuberculosis. The aim of the present study was to compare the direct and indirect MIRU-VNTR genotyping and detection of polyclonal infections between old ...
Mansour Kargarpour Kamakoli   +6 more
openaire   +2 more sources

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