Results 101 to 110 of about 145,034 (286)
Enterobacter bugandensis: a novel enterobacterial species associated with severe clinical infection [PDF]
Nosocomial pathogens can cause life-threatening infections in neonates and immunocompromised patients. E. bugandensis (EB-247) is a recently described species of Enterobacter, associated with neonatal sepsis.
Bunk, Boyke +20 more
core +2 more sources
Association Between D‐amino Acids in Natto (fermented soybeans) and Kidney Dysfunction
XX XX ABSTRACT In this study, we examined clinical and cohort data to explore the potential renoprotective effects of natto (fermented soybeans) consumption and its relationship with D‐amino acids. In a clinical study, ten healthy adults consumed one 40‐g pack of natto three times in a single day, and plasma and urine samples were collected 1 h and 24 ...
Yusuke Nakade +19 more
wiley +1 more source
Environmental DNA (eDNA) analysis is a method of detecting DNA from environmental samples and is used as a biomonitoring tool. In recent studies, Illumina MiSeq has been the most extensively used tool for eDNA metabarcoding.
Ryohei Nakao +5 more
doaj +1 more source
RNA-binding protein CPEB1 remodels host and viral RNA landscapes. [PDF]
Host and virus interactions occurring at the post-transcriptional level are critical for infection but remain poorly understood. Here, we performed comprehensive transcriptome-wide analyses revealing that human cytomegalovirus (HCMV) infection results in
Aigner, Stefan +17 more
core +2 more sources
Analysis of hypervariable DNA sequences by NGS technologies: QuasiFlow [PDF]
The development of Next Generation Sequencing (NGS) technologies has allowed deep characterization of highly variable sequences such as viral or mitochondrial genomes.
Claros-Diaz, Manuel Gonzalo +4 more
core
Complete genome sequences of three African foot-and-mouth disease viruses from clinical samples isolated in 2009 and 2010 [PDF]
<p>The complete genome sequences of three foot-and-mouth disease viruses (one virus of each serotype SAT1, SAT2 and O) were directly sequenced from RNA extracted from clinical bovine samples, demonstrating the feasibility of full-genome sequencing ...
De Clercq, Kris +8 more
core +1 more source
Primer ID MiSeq Library Prep v2
This is the protocol to prepare Primer ID MiSeq sequencing library. Viral RNA was first extracted using QIAamp viral RNA extraction kit. The block of random nucleotides (Ns) in the cDNA primers served as the Primer ID. The Superscript III kit was used for the cDNA synthesis. We used two rounds of PCR to amplify the cDNA and incorporate Illumina indexed
openaire +1 more source
cpn60 metagenomic amplicon library preparation for the Illumina Miseq platform
Abstract This protocol can be applied to determine the composition of a microbial community. The cpn60 gene (also known as groEL, hsp60) is present in almost all bacteria and a 552-558 bp region of the gene has been established as a barcode for species level identification of bacteria. The primer cocktail used in this
Champika Fernando, Janet E. Hill
openaire +1 more source
Abstract Objective This study investigated the relationship between the consumption of ultra‐processed foods (UPF), dietary profile, and inflammation on the intestinal microbiome in children. Methods A cross‐sectional study was conducted using data from a community‐based controlled trial involving 82 children aged 7–11 years enrolled in public schools ...
Cristiane Cosmo Silva‐Luis +3 more
wiley +1 more source
Targeted fetal cell‐free DNA screening for aneuploidies in 4,594 pregnancies: Single center study
Background Next‐generation sequencing (NGS) and discovery of fetal cell‐free DNA (cfDNA) in the maternal circulation render possible prenatal screening for trisomy 21 (Down syndrome), trisomy 18, trisomy 13, and sex chromosome aneuploidies.
Altug Koc +17 more
doaj +1 more source

