Results 101 to 110 of about 147,075 (286)

Analysis of hypervariable DNA sequences by NGS technologies: QuasiFlow [PDF]

, 2016
The development of Next Generation Sequencing (NGS) technologies has allowed deep characterization of highly variable sequences such as viral or mitochondrial genomes.
Claros-Diaz, Manuel Gonzalo, Díaz Martínez, Luis, Grande-Perez, Ana, Seoane Zonjic, Pedro, Viguera-Minguez, Enrique   +4 more
core  

Arbuscular mycorrhizal fungal community abundance, functions, and symbiotic interactions revealed by root metatranscriptomes

iMetaOmics, EarlyView.
Paradigm shift: PCR‐free methods reveal 6–15‐fold higher arbuscular mycorrhizal (AM) fungal abundance than metabarcoding, exposing systematic underestimation across decades of research. Predictive power: AM fungal abundance serves as a community‐level trait that predicts crop yield under drought conditions.
Peilin Chen, John W. Taylor, Cheng Gao
wiley   +1 more source

Complete genome sequences of three African foot-and-mouth disease viruses from clinical samples isolated in 2009 and 2010 [PDF]

, 2016
<p>The complete genome sequences of three foot-and-mouth disease viruses (one virus of each serotype SAT1, SAT2 and O) were directly sequenced from RNA extracted from clinical bovine samples, demonstrating the feasibility of full-genome sequencing ...
De Clercq, Kris, Fana, Mpolokang Elliot, Haegeman, Andy, Hyera, Joseph, Matlho, George, Rosseel, Toon, Seoke, Latoa, Van Borm, Steven, Vandenbussche, Frank   +8 more
core   +1 more source

Mapping the subgingival HerBiome and HisBiome over the human healthspan

Journal of Periodontology, EarlyView.
Abstract Background Understanding the intricate relationship between sex, age, and the oral microbiome is crucial for deciphering the onset and progression of numerous age‐related oral and systemic diseases. Methods Subgingival plaque was collected from 781 periodontally and systemically healthy females and 160 males spanning 0 to 80 years.
Rahul Nikam, Kazune Pax, Michelle Lee‐Scott Beverly, Purnima S. Kumar   +3 more
wiley   +1 more source

Primer ID MiSeq Library Prep v2

, 2017
This is the protocol to prepare Primer ID MiSeq sequencing library. Viral RNA was first extracted using QIAamp viral RNA extraction kit. The block of random nucleotides (Ns) in the cDNA primers served as the Primer ID. The Superscript III kit was used for the cDNA synthesis. We used two rounds of PCR to amplify the cDNA and incorporate Illumina indexed
openaire   +1 more source

cpn60 metagenomic amplicon library preparation for the Illumina Miseq platform

, 2021
Abstract This protocol can be applied to determine the composition of a microbial community. The cpn60 gene (also known as groEL, hsp60) is present in almost all bacteria and a 552-558 bp region of the gene has been established as a barcode for species level identification of bacteria. The primer cocktail used in this
Champika Fernando, Janet E. Hill
openaire   +1 more source

Comparative analysis of fungal microbiomes in Korean and Chinese chili pepper powders

Journal of the Science of Food and Agriculture, EarlyView.
Abstract BACKGROUND Chili pepper powder plays a crucial role in kimchi fermentation; however, its use may result in fungal contamination. Therefore, analyzing its mycobiome is vital to maintain quality control standards. In this study, we used internal transcribed spacer (ITS)2 amplicon sequencing to compare fungal communities in commercially available
Jin‐Hee Seo, Ji‐Hyoung Ha, Su‐Hyeon Cho, Kil‐Nam Kim, Hae‐Won Lee   +4 more
wiley   +1 more source

Targeted fetal cell‐free DNA screening for aneuploidies in 4,594 pregnancies: Single center study

Molecular Genetics & Genomic Medicine, 2019
Background Next‐generation sequencing (NGS) and discovery of fetal cell‐free DNA (cfDNA) in the maternal circulation render possible prenatal screening for trisomy 21 (Down syndrome), trisomy 18, trisomy 13, and sex chromosome aneuploidies.
Altug Koc, Ozge Ozer Kaya, Berk Ozyilmaz, Yasar B. Kutbay, Ozgur Kirbiyik, Taha R. Ozdemir, Kadri M. Erdogan, Merve Saka Guvenc, Deniz C. Oztekin, Mehmet Ozeren, Halil G. Pala, Atalay Ekin, Cenk Gezer, Alkim G. Sahingoz Yildirim, Bahar Konuralp Atakul, Secil Kurtulmus, Ugur Turhan, Cuneyt E. Taner   +17 more
doaj   +1 more source

Primer ID MiSeq Library Prep v1

, 2017
This is the protocol to prepare Primer ID MiSeq sequencing library. Viral RNA was first extracted using QIAamp viral RNA extraction kit. The block of random nucleotides (Ns) in the cDNA primers served as the Primer ID. The Superscript III kit was used for the cDNA synthesis. We used two rounds of PCR to amplify the cDNA and incorporate Illumina indexed
openaire   +1 more source

Enterobacter bugandensis: a novel enterobacterial species associated with severe clinical infection [PDF]

, 2018
Nosocomial pathogens can cause life-threatening infections in neonates and immunocompromised patients. E. bugandensis (EB-247) is a recently described species of Enterobacter, associated with neonatal sepsis.
Bunk, Boyke, Chakraborty, Trinad, Doijad, Swapnil Prakash, Falgenhauer, Linda, Goesmann, Alexander, Gwozdzinski, Konrad, Hain, Torsten, Hegemann, Julian D., Imirzalioglu, Can, Jaiswal, Sangeeta, Mannala, Gopala Krishna, Marahiel, Mohamed A., Mraheil, Mobarak Abu, Mshana, Stephen E., Overmann, Jörg, Pati, Niladri Bhusan, Ryan, Daniel, Schultze, Tilman, Spröer, Cathrin, Suar, Mrutyunjay, Yao, Yancheng   +20 more
core   +2 more sources