Results 51 to 60 of about 147,075 (286)

Insights into bacterioplankton community structure from Sundarbans mangrove ecoregion using Sanger and Illumina MiSeq sequencing approaches: A comparative analysis

Genomics Data, 2017
Next generation sequencing using platforms such as Illumina MiSeq provides a deeper insight into the structure and function of bacterioplankton communities in coastal ecosystems compared to traditional molecular techniques such as clone library approach ...
Anwesha Ghosh, Punyasloke Bhadury
doaj   +1 more source

Effects of wheat root exudates on bacterial communities in the rhizosphere of watermelon

Plant, Soil and Environment, 2021
In this study, we investigated the effects of wheat root exudates on soil bacterial communities in the watermelon rhizosphere using quantitative PCR and Illumina MiSeq sequencing.
Jibo Shi, Xiaoya Gong, Muhammad Khashi u Rahman, Qing Tian, Xingang Zhou, Fengzhi Wu   +5 more
doaj   +1 more source

Comparative next-generation sequencing of adeno-associated virus inverted terminal repeats

BioTechniques, 2014
The inverted terminal repeats (ITRs) of adeno-associated virus (AAV) are notoriously difficult to sequence owing to their high GC-content (70%) and palindromic sequences that result in the formation of a very stable, 125 bp long, T-shaped hairpin ...
Karl Petri, Raffaele Fronza, Richard Gabriel, Christine Käppel, Ali Nowrouzi, R. Michael Linden, Els Henckaerts, Manfred Schmidt   +7 more
doaj   +1 more source

Transmitting silks of maize have a complex and dynamic microbiome

Scientific Reports, 2021
In corn/maize, silks emerging from cobs capture pollen, and transmit resident sperm nuclei to eggs. There are > 20 million silks per U.S. maize acre.
Eman M. Khalaf, Anuja Shrestha, Jeffrey Rinne, Michael D. J. Lynch, Charles R. Shearer, Victor Limay-Rios, Lana M. Reid, Manish N. Raizada   +7 more
doaj   +1 more source

Next-generation sequencing-based multigene mutational screening for acute myeloid leukemia using MiSeq: applicability for diagnostics and disease monitoring

Haematologica, 2014
Routine molecular testing in acute myeloid leukemia involves screening several genes of therapeutic and prognostic significance for mutations. A comprehensive analysis using single-gene assays requires large amounts of DNA, is cumbersome and timely ...
Rajyalakshmi Luthra, Keyur P. Patel, Neelima G. Reddy, Varan Haghshenas, Mark J. Routbort, Michael A. Harmon, Bedia A. Barkoh, Rashmi Kanagal-Shamanna, Farhad Ravandi, Jorge E Cortes, Hagop M Kantarjian, L. Jeffrey Medeiros, Rajesh R. Singh   +12 more
doaj   +1 more source

Comparative analysis of targeted next-generation sequencing for Plasmodium falciparum drug resistance markers

Scientific Reports, 2022
Well-defined molecular resistance markers are available for a range of antimalarial drugs, and molecular surveillance is increasingly important for monitoring antimalarial drug resistance.
Chanon Kunasol, Arjen M. Dondorp, Elizabeth M. Batty, Vorthunju Nakhonsri, Puritat Sinjanakhom, Nicholas P. J. Day, Mallika Imwong   +6 more
doaj   +1 more source

Primer ID MiSeq Library Prep v4 [PDF]

, 2018
This is the protocol to prepare Primer ID MiSeq sequencing library. Viral RNA was first extracted using QIAamp viral RNA extraction kit. The block of random nucleotides (Ns) in the cDNA primers served as the Primer ID. The Superscript III kit was used for the cDNA synthesis. We used two rounds of PCR to amplify the cDNA and incorporate Illumina indexed
openaire   +1 more source

Genomic catastrophes frequently arise in esophageal adenocarcinoma and drive tumorigenesis [PDF]

, 2014
Oesophageal adenocarcinoma (EAC) incidence is rapidly increasing in Western countries. A better understanding of EAC underpins efforts to improve early detection and treatment outcomes.
Anderson, M, Bailey, P, Barbour, A, Bruxner, T, Campbell, P, Christ, A, Fink, J, Gotley, D, Grimmond, S, Harliwong, I, Hayward, N, Holmes, O, Hussey, D, Idrisoglu, S, Kazakoff, S, Krause, L, Lampe, G, Leonard, C, Loffler, K, Lord, R, Manning, S, Miller, D, Nancarrow, D, Newell, F, Nones, K, Nourbakhsh, E, Nourse, C, Patch, A, Pearson, J, Phillips, W, Quek, K, Quinn, M, Reid, L, Simpson, P, Smithers, B, Tang, Y, Waddell, N, Waddell, N, Watson, D, Wayte, N, Whiteman, D, Wilson, P, Wood, S, Xu, Q   +43 more
core   +4 more sources

Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications

Advanced Science, EarlyView.
Classical transfection and transduction are inefficient, particularly with confluent cells and organoids, and lack cell type‐specific programmability. This study presents calcium shock (CaSh), a method that dramatically improves particle delivery into single cells, colonies, and organoids.
Nicole Vo, Lorena de Oñate, Maximillian Frank, Xian Hu, Sophie M. Blackburn, Dana V. Foss, Jasmine Villegas, Kunica Asija, Dirk Hockemeyer, Hongxia Fu, Raymond J. Monnat, Ross C. Wilson, Benjamin S. Freedman   +12 more
wiley   +1 more source

Rapid Remodeling of the Human Gut Microbiome in Response to Short‐Term Animal Product Restriction and Associations with Host Molecular Phenotypes

Advanced Science, EarlyView.
A real‐world model of structured animal product restriction practiced for religious reasons reveals the dynamic adaptability of the human gut microbiome to dietary change and uncovers reductions in diversity and rare taxa loss. Integrated microbiome, metabolomic, and proteomic analyses uncover coordinated taxonomic and molecular shifts and identify ...
Christina Emmanouil, Maria Anezaki, Alexandros Simistiras, Stavros Glentis, Nikolaos Scarmeas, Pantelis Hatzis, Konstantinos Rouskas, Antigone S. Dimas   +7 more
wiley   +1 more source