Results 41 to 50 of about 66,055 (244)

A reversible histone H3 acetylation cooperates with mismatch repair and replicative polymerases in maintaining genome stability. [PDF]

PLoS Genetics, 2013
Mutations are a major driving force of evolution and genetic disease. In eukaryotes, mutations are produced in the chromatin environment, but the impact of chromatin on mutagenesis is poorly understood.
Lyudmila Y Kadyrova, Tony M Mertz, Yu Zhang, Matthew R Northam, Ziwei Sheng, Kirill S Lobachev, Polina V Shcherbakova, Farid A Kadyrov   +7 more
doaj   +1 more source

Paleo sea-level indicators and proxies from Greenland in the GAPSLIP database and comparison with modelled sea level from the PaleoMIST ice-sheet reconstruction

GEUS Bulletin, 2023
One of the most common ways to assess ice-sheet reconstructions of the past is to evaluate how they impact changes in sea level through glacial isostatic adjustment. PaleoMIST 1.0, a preliminary reconstruction of topography and ice sheets during the past
Evan J. Gowan
doaj   +1 more source

Theoretical study on enhancement of DNA conductivity by copper modification of mismatched base pairs

Journal of Physics: Conference Series, 2019
Abstract DNA is a potential material for nanodevices. Metallization is a promising way to improve its conductivity. Two copper modified mismatched base pairs were designed by H-by-Cu replacement. The conductivities of these base pairs were studied by density functional theory (DFT) combined with nonequilibrium Green’s function (NEGF ...
Haiying Liu, Deqi Yang, Yigeng Tian, Yingying Cheng   +3 more
openaire   +1 more source

Longitudinal genome‐wide aneuploidy measurements in circulating cell‐free DNA to predict lack of benefit from pembrolizumab in patients with metastatic urothelial cancer

Molecular Oncology, EarlyView.
Many patients with urothelial cancer do not benefit from treatment with pembrolizumab, while at risk of severe side effects. Changes in the levels of circulating tumor DNA early during treatment, measured by a simple and affordable assay that can be easily implemented in the clinic, can be used as a prognostic tool to identify these patients.
Youssra Salhi, Stavros Makrodimitris, Sandra van Wilpe, Iris te Paske, Vanja de Weerd, Corine M. Beaufort, Hans M. Westgeest, Jens Voortman, Maureen J. B. Aarts, Maud Rijnders, Astrid A. M. van der Veldt, Ronald de Wit, Niven Mehra, Saskia M. Wilting, Debbie G. J. Robbrecht   +14 more
wiley   +1 more source

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

Epigenetic heterogeneity and plasticity in therapy‐induced tumor states through single‐cell multi‐omics

Molecular Oncology, EarlyView.
Single‐cell multi‐omics reveals epigenetic heterogeneity across therapy‐adaptive tumor states, including quiescent/dormant, drug‐tolerant persister, and EMT‐like phenotypes. By linking regulatory features with state‐associated biomarkers, these approaches inform biomarker‐guided therapeutic strategies for evolving tumors.
Hee Jung Kim, Hwiyeong Lee, Jin Hong, Daechan Park   +3 more
wiley   +1 more source

Detection of single base-pair mismatches in DNA by chemical modification followed by electrophoresis in 15% polyacrylamide gel. [PDF]

Proceedings of the National Academy of Sciences, 1986
We have developed a method for distinguishing fragments of DNA that contain single-base mismatches from their perfectly paired homologues. Single-stranded regions within a duplex fragment are accessible to 1-cyclohexyl-3-(2-[4-(4-methyl)morpholinyl]ethyl)carbodiimide, which reacts with unpaired guanidylate and thymidylate residues in DNA. Intact linear
D F, Novack, N J, Casna, S G, Fischer, J P, Ford   +3 more
openaire   +2 more sources

Cancer cell‐intrinsic type 1 interferon: production, signaling, and outcomes within sex‐biased, female malignancies

Molecular Oncology, EarlyView.
The cell‐autonomous roles of interferon type 1 vary based on duration and intensity. While acute, robust signaling results in cancer cell cytotoxic effects, sustained, low‐level signaling is associated with tumor‐promoting effects. This review summarizes current research of IFN‐1 in cancer and within the clinical setting, with an emphasis on female ...
Ashlyn Conant, Nora Badiner, Sharon Asariah, Kiera McGivney, Vishwa Shah, Tise Suzuki, Yevgeniya J. Ioffe, Juli J. Unternaehrer   +7 more
wiley   +1 more source

Machining calculation and tooth surface modification method of circular arc equal-height spiral bevel gear based on line conjugation

Journal of Advanced Mechanical Design, Systems, and Manufacturing
In order to accurately guide the tooth cutting of circular arc equal-height spiral bevel gear and improve its meshing performance, an analytical calculation method of processing parameters based on the line conjugation principle and a tooth surface ...
Shaowu NIE, Chuang JIANG, Zhengyang HAN, Longlong GENG   +3 more
doaj   +1 more source

Extensive trimming of short single-stranded DNA oligonucleotides during replication-coupled gene editing in mammalian cells.

PLoS Genetics, 2020
Through transfection of short single-stranded oligodeoxyribonucleotides (ssODNs) small genomic alterations can be introduced into mammalian cells with high precision. ssODNs integrate into the genome during DNA replication, but the resulting heteroduplex
Thomas W van Ravesteyn, Marcos Arranz Dols, Wietske Pieters, Marleen Dekker, Hein Te Riele   +4 more
doaj   +1 more source