Results 81 to 90 of about 1,007,169 (424)
Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis [PDF]
Methods in molecular biology (Clifton, N.J.) 432 (2008) 83-100, 2008 Mitochondria are complex organelles, and their proteomics analysis requires a combination of techniques. The emphasis in this chapter is made first on mitochondria preparation from cultured mammalian cells, then on the separation of the mitochondrial proteins with two-dimensional electrophoresis (2DE), showing some adjustment over the classical ...
arxiv +1 more source
Assembly of mitochondria [PDF]
, 1980 The majority of mitochondrial proteins are synthesized on cytoplasmic ribosomes and transferred to the mitochondria where they are assembled to supramolecular structures.
Harmey, Matthew A. +3 more
core +1 more source
Clinical and Experimental Reproductive Medicine, 2023 In reproduction, mitochondria produce bioenergy, help to synthesize biomolecules, and support the ovaries, oogenesis, and preimplantation embryos, thereby facilitating healthy live births. However, the regulatory mechanism of mitochondria in oocytes and embryos during oogenesis and embryo development has not been clearly elucidated.
Min-Hee Kang, Yu Jin Kim, Jae Ho Lee
openaire +2 more sources
BMC Bioinformatics, 2020 Background Mass spectrometry (MS) has become a promising analytical technique to acquire proteomics information for the characterization of biological samples.
Shisheng Wang +4 more
doaj +1 more source
Mitochondrial Ca2+ uptake in skeletal muscle health and disease [PDF]
, 2016 Muscle uses Ca2+ as a messenger to control contraction and relies on ATP to maintain the intracellular Ca2+ homeostasis. Mitochondria are the major sub-cellular organelle of ATP production. With a negative inner membrane potential, mitochondria take up Ca2+ from their surroundings, a process called mitochondrial Ca2+ uptake.
arxiv +1 more source
MiShape: 3D Shape Modelling of Mitochondria in Microscopy [PDF]
arXiv, 2023 Fluorescence microscopy is a quintessential tool for observing cells and understanding the underlying mechanisms of life-sustaining processes of all living organisms. The problem of extracting 3D shape of mitochondria from fluorescence microscopy images remains unsolved due to the complex and varied shapes expressed by mitochondria and the poor ...
arxiv
Deletion of heat shock protein 60 in adult mouse cardiomyocytes perturbs mitochondrial protein homeostasis and causes heart failure. [PDF]
, 2020 To maintain healthy mitochondrial enzyme content and function, mitochondria possess a complex protein quality control system, which is composed of different endogenous sets of chaperones and proteases.
Chen, Ju +13 more
core
Nature Neuroscience, 2019 In neurodegenerative diseases, debris of dead neurons are thought to trigger glia-mediated neuroinflammation, thus increasing neuronal death. Here we show that the expression of neurotoxic proteins associated with these diseases in microglia alone is ...
Amit U. Joshi +6 more
semanticscholar +1 more source
Cancer & Metabolism, 2014 Editorial Proliferating cells such as tumor cells have increased metabolic demands that include ATP, NADPH, lipids, proteins, and nucleotides to allow for a tumor cell to divide into two daughter cells [1]. Tumor cells reprogram their cell metabolism to sustain the increased metabolic demands of cell proliferation.
openaire +3 more sources
3D Mitochondria Instance Segmentation with Spatio-Temporal Transformers [PDF]
arXiv, 2023 Accurate 3D mitochondria instance segmentation in electron microscopy (EM) is a challenging problem and serves as a prerequisite to empirically analyze their distributions and morphology. Most existing approaches employ 3D convolutions to obtain representative features.
arxiv