Results 61 to 70 of about 4,754,428 (281)

Development of a Finite Element Methodology for Modelling Mixed-Mode Delamination Growth [PDF]

, 2007
A critical failure mechanism for composite skin-stiffened structures in compression is separation of the skin and stiffener, which can be considered analogous to interlaminar cracking.
Degenhardt, Richard, Bayandor, J., Thomson, R.S., Orifici, Adrian, Büsing, S.   +4 more
core  

Synthetic oligonucleotide separations by mixed-mode reversed-phase/weak anion-exchange liquid chromatography

, 2014
Synthetic oligonucleotides gain increasing importance in new therapeutic concepts and as probes in biological sciences. If pharmaceutical-grade purities are required, chromatographic purification using ion-pair reversed-phase chromatography is commonly ...
Isabel Walker, Michael Lämmerhofer, GRECO, Roberto, CAVAZZINI, Alberto, Aleksandra Zimmermann, Jeannie Horak   +5 more
core   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

Determination of eight selenium species in selenium-enriched edible oil with accelerated solvent extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry

Shipin yu jixie
ObjectiveIn this study, an optimized strategy was proposed to address the limitations of current methods for detecting selenium species in selenium-enriched edible oils, such as the transformation between selenium species, long enzymatic hydrolysis time,
LIANG Zhisen, ZHANG Shudi, LING Xiao, CHEN Yuzhen   +3 more
doaj   +1 more source

Preparative isolation of PCR products using mixed-mode chromatography.

, 2015
The polymerase chain reaction (PCR) has become one of the most useful techniques in molecular biology laboratories around the world. The purification of the target DNA product is, however, often challenging and most users are restricted to employing ...
Bülow, Leif, Matos, Tiago, Queiroz, J A, Dos Santos Silva, Graciela   +3 more
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Determination of Trimetazidine and Clomiphene in Animal-Derived Foods by Mixed-Mode Reversed-Phase Strong Cation Exchange Solid Phase Extraction Cleanup Followed by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry [PDF]

Shipin Kexue
A method for the determination of trimetazidine and clomiphene in animal-derived foods was developed by mixed-mode reversed-phase strong cation exchange solid phase extraction cleanup followed by ultra-high performance liquid chromatography-tandem mass ...
LU Lanxiang, XUE Xia, TIAN Cui, ZHANG Yanxia, SU Shufang, LI Xinling, LIU Yanming, WANG Jun, HU Mei
doaj   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

FEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source

Hydrophobicity of various cation-exchange columns for ion chromatography

Journal of Chromatography Open
The retention of organic cations including homologues of n-alkylamines, phenylalkylamines, and ethanolamines was investigated for 20 commercially produced cation-exchange columns with varying matrix, bonding chemistry, functional groups, and ion-exchange
Pavel N. Nesterenko, Ekaterina P. Nesterenko   +1 more
doaj   +1 more source