Results 41 to 50 of about 4,947,794 (321)

Iron Oxide Nanoparticles: Multiwall Carbon Nanotube Composite Materials for Batch or Chromatographic Biomolecule Separation

Nanoscale Research Letters, 2021
Carbon-based materials are the spearhead of research in multiple fields of nanotechnology. Moreover, their role as stationary phase in chromatography is gaining relevance.
Sebastian P. Schwaminger, Markus W. Brammen, Florian Zunhammer, Nicklas Däumler, Paula Fraga-García, Sonja Berensmeier   +5 more
doaj   +1 more source

Homochiral Metal-Organic Framework Based Mixed Matrix Membrane for Chiral Resolution

Membranes, 2022
Efficient separation of enantiomers is critical in the chemical, pharmaceutical, and food industries. However, conventional separation methods, such as chromatography, crystallization, and enzymatic kinetic resolution, require high energy costs and ...
Hwa-Jin Choi, Dong-Yeun Koh
doaj   +1 more source

Isolation of amyloidogenic aggregates of transthyretin by ceramic hydroxyapatite chromatography

Journal of Chromatography Open
Amyloidogenic proteins can lapse into unstable monomeric precursors which misfold and self-assemble into toxic oligomers and amyloid aggregates leading to intracellular deposition of insoluble amyloid fibrils, the hallmark of amyloidosis and major ...
Maria K. Tsoumpra, Shintaro Kobayashi, Eri Chatani   +2 more
doaj   +1 more source

Preparative isolation of PCR products using mixed-mode chromatography.

, 2015
The polymerase chain reaction (PCR) has become one of the most useful techniques in molecular biology laboratories around the world. The purification of the target DNA product is, however, often challenging and most users are restricted to employing ...
Bülow, Leif, Matos, Tiago, Queiroz, J A, Dos Santos Silva, Graciela, Dos Santos Silva, Graciela,, Bülow, Leif,, Matos, Tiago,, Lund University.   +7 more
core   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Ion exchange chromatography – basic principles and application [PDF]

, 2011
Ion-Exchange Chromatography (IEC) allows for the separation of ionizable molecules on the basis of differences in charge properties. Its large sample-handling capacity, broad applicability (particularly to proteins and enzymes), moderate cost, powerful ...
Dowling, Oonagh, Cummins, Phil, O'Connor, Brendan   +2 more
core   +2 more sources

Synthetic oligonucleotide separations by mixed-mode reversed-phase/weak anion-exchange liquid chromatography

, 2014
Synthetic oligonucleotides gain increasing importance in new therapeutic concepts and as probes in biological sciences. If pharmaceutical-grade purities are required, chromatographic purification using ion-pair reversed-phase chromatography is commonly ...
Isabel Walker, Michael Lämmerhofer, GRECO, Roberto, CAVAZZINI, Alberto, Aleksandra Zimmermann, Jeannie Horak   +5 more
core   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Mixed-Mode Chromatography: Studies on Hybrid Retention Mechanisms of Some Antihypertensive Drugs

Separations
The antihypertensive drugs indapamide, atenolol, metoprolol, propranolol and bisoprolol were considered in this research. Because they have structures that are affected by pH, developing a chromatographic method was challenging.
Irinel Adriana Badea, Andrei Mihăilă, Dana Elena Popa, Anca Monica Tencaliec, Mihaela Buleandră   +4 more
doaj   +1 more source