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Perspective Chapter: Mixed-Mode Chromatography

, 2022
In this chapter, we present mixed-mode stationary phases and their applications in the determination of nonpolar, polar, and charged compounds, as well as larger molecules such as peptides or proteins using a single column. Mixed-mode chromatography (MMC) has been growing rapidly in recent years, owing to the new generation of mixed-mode stationary ...
openaire   +1 more source

Use of Commercial Mixed-Mode Stationary Phases and Sorbents in the High-Performance Liquid Chromatography Analysis and Solid-Phase Extraction of Ionized and Hydrophilic Bioactive Compounds

Molecules
Mixed-mode high-performance liquid chromatography (HPLC) is increasingly used for the analysis of ionic and highly hydrophilic drugs, which are difficult to separate by conventional single-mode HPLC.
Takeshi Fukushima, Mikoto Koishi, Tatsuya Sakamoto, Mayu Onozato   +3 more
doaj   +1 more source

Determination of polycyclic aromatic hydrocarbons in soil by gas chromatography-mass spectrometry [PDF]

Acta Periodica Technologica, 2005
Polycyclic aromatic hydrocarbons (PAHs) were determined in soil samples from forty different locations in Vojvodina. The samples were Soxhlet extracted cleaned-up and analyzed using gas chromatography-mass spectrometry (GC-MS) in the selected ion ...
Kravić Snežana Ž., Marjanović Nikola J., Pucarević Mira M., Suturović Zvonimir J., Švarc-Gajić Jaroslava V.   +4 more
doaj   +1 more source

Monoclonal antibody (mAb) purification by counter current chromatography (CCC) [PDF]

, 2011
This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.Counter current chromatography (CCC) is a form of liquid liquid chromatography, which the Brunel Institute for Bioengineering (BIB) team have developed to ...
Fernando, Samantha
core   +7 more sources

Iron Oxide Nanoparticles: Multiwall Carbon Nanotube Composite Materials for Batch or Chromatographic Biomolecule Separation

Nanoscale Research Letters, 2021
Carbon-based materials are the spearhead of research in multiple fields of nanotechnology. Moreover, their role as stationary phase in chromatography is gaining relevance.
Sebastian P. Schwaminger, Markus W. Brammen, Florian Zunhammer, Nicklas Däumler, Paula Fraga-García, Sonja Berensmeier   +5 more
doaj   +1 more source

Homochiral Metal-Organic Framework Based Mixed Matrix Membrane for Chiral Resolution

Membranes, 2022
Efficient separation of enantiomers is critical in the chemical, pharmaceutical, and food industries. However, conventional separation methods, such as chromatography, crystallization, and enzymatic kinetic resolution, require high energy costs and ...
Hwa-Jin Choi, Dong-Yeun Koh
doaj   +1 more source

Isolation of amyloidogenic aggregates of transthyretin by ceramic hydroxyapatite chromatography

Journal of Chromatography Open
Amyloidogenic proteins can lapse into unstable monomeric precursors which misfold and self-assemble into toxic oligomers and amyloid aggregates leading to intracellular deposition of insoluble amyloid fibrils, the hallmark of amyloidosis and major ...
Maria K. Tsoumpra, Shintaro Kobayashi, Eri Chatani   +2 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Curve fitting of mixed-mode isopachics

, 2009
Recent work has focused on exploiting the observation that the stress-sum contours (isopachics), obtained from TSA, in the vicinity of the tip take the form of a simple curve – the cardioid.
Barton, Janice .M., Tatum, P., Worden, K., Hebb, R.I., Dulieu-Barton, J.M.   +4 more
core   +1 more source