Results 31 to 40 of about 4,947,794 (321)

The effect of kosmotropic salts on aggregate removal from monoclonal antibodies in mixed-mode anion exchange chromatography

Current Research in Biotechnology, 2023
Efficacious and safe application of recombinant proteins in humans as drugs depends on their high purity accomplished by downstream processing. The present study evaluates the effects of kosmotropic salts used in the flow-through mode of mixed-mode anion
Susanne Konrad, Tobias Graf, Roberto Falkenstein   +2 more
doaj   +1 more source

Glutathione-based zwitterionic stationary phase for hydrophilicinteractioncation-exchange mixed-mode chromatography

, 2013
Glutathione-based zwitterionic stationary phase for hydrophilicinteractioncation-exchange mixed-mode ...
李秀玲, 魏杰, 梁鑫淼, 沈爱金, 郭志谋, 董雪芳   +5 more
core   +2 more sources

A New Definition of the Stationary Phase Volume in Mixed-Mode Chromatographic Columns in Hydrophilic Liquid Chromatography

Molecules, 2021
Polar columns used in the HILIC (Hydrophilic Interaction Liquid Chromatography) systems take up water from the mixed aqueous–organic mobile phases in excess of the water concentration in the bulk mobile phase.
Pavel Jandera, Tomáš Hájek
doaj   +1 more source

Harnessing ceramic hydroxyapatite as an effective polishing strategy to remove product- and process-related impurities in bispecific antibody purification

Bioresources and Bioprocessing, 2023
Bispecific antibody (bsAb), a novel therapeutic modality, provides excellent treatment efficacy, yet poses numerous challenges to downstream process development, which are mainly due to the intricate diversity of bsAb structures and impurity profiles ...
Nattha Ingavat, Xinhui Wang, Jia Min Liew, Farouq Bin Mahfut, Ka Pui But, Yee Jiun Kok, Xuezhi Bi, Yuansheng Yang, Kobayashi Shintaro, Maria Tsoumpra, Wei Zhang   +10 more
doaj   +1 more source

Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica

Molecules, 2016
The use of a liquid chromatography (LC) splitter inserted between an HPLC column and an evaporative light scattering detector (ELSD) is described. This paper aims to show the feasibility of using the splitter in an HPLC-ELSD system to fractionate a model
Jiří Pazourek, Karel Šmejkal
doaj   +1 more source

Isolation, Structural Identification and in Vitro Activity Evaluation of Angiotensin-Converting Enzyme Inhibitory Peptides from Moringa oleifera Seeds [PDF]

Shipin Kexue, 2023
In this study, angiotensin-converting enzyme (ACE) inhibitory peptides from an enzymatic hydrolysate of Moringa oleifera seeds were separated by sequential ultrafiltration and ion exchange chromatography.
ZHONG Yuwang, XU Wanli, FAN Yaozhu, LI Yiyan, WANG Xuefeng
doaj   +1 more source

Preparation of mixed-mode stationary phase for separation of peptides and proteins in high performance liquid chromatography

Scientific Reports, 2022
Porous silica particles were prepared by sol–gel method with some modification to get wide-pore particles. These particles were derivatized with N-phenylmaleimide-methylvinylisocyanate (PMI) and styrene by reversible addition fragmentation chain transfer
Sarah Alharthi, Ashraf Ali, Muzaffar Iqbal, Aliya Ibrar, Bashir Ahmad, Sobia Nisa, Fazal Mabood   +6 more
doaj   +1 more source

Solid-Phase Extraction Approaches for Improving Oligosaccharide and Small Peptide Identification with Liquid Chromatography-High-Resolution Mass Spectrometry: A Case Study on Proteolyzed Almond Extract

Foods, 2022
Reverse-phase solid-phase extraction (SPE) is regularly used for separating and purifying food-derived oligosaccharides and peptides prior to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.
Yu-Ping Huang, Randall C. Robinson, Fernanda Furlan Goncalves Dias, Juliana Maria Leite Nobrega de Moura Bell, Daniela Barile   +4 more
doaj   +1 more source

Perspective Chapter: Mixed-Mode Chromatography

, 2022
In this chapter, we present mixed-mode stationary phases and their applications in the determination of nonpolar, polar, and charged compounds, as well as larger molecules such as peptides or proteins using a single column. Mixed-mode chromatography (MMC) has been growing rapidly in recent years, owing to the new generation of mixed-mode stationary ...
openaire   +1 more source

Determination of polycyclic aromatic hydrocarbons in soil by gas chromatography-mass spectrometry [PDF]

Acta Periodica Technologica, 2005
Polycyclic aromatic hydrocarbons (PAHs) were determined in soil samples from forty different locations in Vojvodina. The samples were Soxhlet extracted cleaned-up and analyzed using gas chromatography-mass spectrometry (GC-MS) in the selected ion ...
Kravić Snežana Ž., Marjanović Nikola J., Pucarević Mira M., Suturović Zvonimir J., Švarc-Gajić Jaroslava V.   +4 more
doaj   +1 more source