Results 11 to 20 of about 4,947,794 (321)

Mixed-mode chromatography-mass spectrometry enables targeted and untargeted screening of carboxylic acids in biological samples.

Analytical Methods, 2022
Carboxylic acids are crucial metabolites in the tricarboxylic acid (TCA) cycle and thus participate in central carbon metabolism (CCM). Research dependent on the analysis of metabolites involved in central carbon metabolism requires fast separation and ...
Ondřej Hodek, Lidia Argemi-Muntadas, A. Khan, T. Moritz   +3 more
semanticscholar   +3 more sources

Use of Commercial Mixed-Mode Stationary Phases and Sorbents in the High-Performance Liquid Chromatography Analysis and Solid-Phase Extraction of Ionized and Hydrophilic Bioactive Compounds

Molecules
Mixed-mode high-performance liquid chromatography (HPLC) is increasingly used for the analysis of ionic and highly hydrophilic drugs, which are difficult to separate by conventional single-mode HPLC.
Takeshi Fukushima, Mikoto Koishi, Tatsuya Sakamoto, Mayu Onozato   +3 more
doaj   +2 more sources

A clinically validated method to separate and quantify underivatized acylcarnitines and carnitine metabolic intermediates using mixed-mode chromatography with tandem mass spectrometry.

Journal of Chromatography A, 2021
Acylcarnitines are intermediate metabolites of the mitochondria that serve as biomarkers for inherited disorders of fatty acid oxidation and amino acid metabolism.
C. Luna, C. Griffin, Marcus J. Miller
semanticscholar   +2 more sources

Mixed‐mode chromatography/isotope ratio mass spectrometry

Rapid Communications in Mass Spectrometry, 2010
Abstract Liquid chromatography coupled to molecular mass spectrometry (LC/MS) has been a standard technique since the early 1970s but liquid chromatography coupled to high‐precision isotope ratio mass spectrometry (LC/IRMS) has only been available commercially since 2004.
McCullagh, JS, James S. O. McCullagh
openaire   +4 more sources

Separation of polar and nonpolar compounds using HILIC/RPLC mixed-mode chromatography: evaluation of separation performance and retention mechanism of the Acclaim Mixed-Mode HILIC-1 column

Open Chemistry
The Acclaim Mixed-Mode HILIC-1 column, combining hydrophilic interaction (HILIC) and reversed-phase liquid chromatography (RPLC) mechanisms, enables efficient separation of diverse analytes.
Aral Hayriye, Dağ Beşir
doaj   +2 more sources

Rapid quantification of underivatized alloisoleucine and argininosuccinate using mixed-mode chromatography with tandem mass spectrometry.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2019
Plasma elevations of the amino acids alloisoleucine and argininosuccinic acid (ASA) are pathognomonic for maple syrup urine disease and argininosuccinate lyase deficiency, respectively.
C. Griffin, Z. Ammous, G. Vance, B. Graham, Marcus J. Miller   +4 more
semanticscholar   +2 more sources

The benefits of mixed-mode chromatography columns for separation of peptides and protein digests

Chemical Monthly, 2023
In this work, the evaluation and comparison of mixed-mode chromatography and reversed-phase chromatography for separation of peptides and protein digests have been performed.
Z. Kadlecová, Hana Boudová, K. Kalíková   +2 more
semanticscholar   +1 more source

Mechanistic modeling, simulation, and optimization of mixed‐mode chromatography for an antibody polishing step

Biotechnology progress (Print), 2022
Mixed‐mode chromatography combines features of ion‐exchange chromatography and hydrophobic interaction chromatography and is increasingly used in antibody purification. As a replacement for flow‐through operations on traditional unmixed resins or as a pH‐
Tobias Hahn, Nora Geng, Katerina Petrushevska-Seebach, Michael E Dolan, Marcus Scheindel, Pia Graf, K. Takenaka, Kyo Izumida, Lijuan Li, Zi-Jian Ma, Norbert Schuelke   +10 more
semanticscholar   +1 more source

High sensitivity acidic N-glycan profiling with MS-enhancing derivatization and mixed mode chromatography.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2022
Acidic N-linked glycan content is often associated with a protein drug's stability, efficacy and immune response. It has often been a challenge to analyze these types of glycans, including those that are differentiated by the incorporation of N-acetyl ...
Xiaoxiao Liu, Qi Wang, M. Lauber
semanticscholar   +1 more source

Data on using single- and mixed-mode resins for capture chromatography of recombinant human thioredoxin from Escherichia coli

Data in Brief, 2020
This paper provides the data collected from screening chromatographic resins for their ability to bind and purify recombinant human thioredoxin from Escherichia coli lysate.
Ayswarya Ravi, Emma Foster, Zivko Nikolov   +2 more
doaj   +1 more source