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Influence of pH value and salts on the adsorption of lysozyme in mixed‐mode chromatography [PDF]
Engineering in Life Sciences, 2021 Mixed‐mode chromatography (MMC) is an interesting technique for challenging protein separation processes which typically combines adsorption mechanisms of ion exchange (IEC) and hydrophobic interaction chromatography (HIC).
Jannette Kreusser +2 more
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Mixed-mode chromatography in pharmaceutical and biopharmaceutical applications [PDF]
Journal of Pharmaceutical and Biomedical Analysis, 2016 Mixed-mode chromatography (MMC) is a fast growing area in recent years, thanks to the new generation of mixed-mode stationary phases and better understanding of multimode interactions. MMC has superior applications in the separation of compounds that are not retained or not well resolved by typical reversed-phase LC methods, especially for polar and ...
Kelly Zhang
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Coupling Mixed Mode Chromatography/ESI Negative MS Detection with Message-Passing Neural Network Modeling for Enhanced Metabolome Coverage and Structural Identification [PDF]
Metabolites, 2021 A key unmet need in metabolomics continues to be the specific, selective, accurate detection of traditionally difficult to retain molecules including simple sugars, sugar phosphates, carboxylic acids, and related amino acids.
Gang Xing +4 more
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Multimodal Anion-Exchange Resins for Chromatographic Separation of Antibody Aggregates [PDF]
BiomoleculesEfficient protein aggregate removal remains a major challenge in downstream bioprocessing because high aggregate clearance must be achieved without compromising monomer yield.
Simona Kotuličová +2 more
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Research Progress in the Separation of Bioactive Peptides Using Mixed-Mode Chromatography [PDF]
Shipin Kexue, 2023 Bioactive peptides are small protein molecules with specific physiological activity. The efficient separation and purification of bioactive peptides contributes to the full exertion of bioactive peptides and research on their structure-activity ...
MA Yueyun, ZHANG Dandan, LI Yingrou, LIU Mengqi, LÜ Rui, LIU Miaomiao, REN Dandan, WU Long, WANG Qiukuan, ZHOU Hui
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Separations, 2022 Peptides are a class of biomolecules with a great potential from the therapeutic point of view, because of their unique biological properties. Industrially, the production stategies adopted produce both the target peptide and a series of impurities that ...
Giulio Lievore +11 more
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Temperature-responsive mixed-mode column for the modulation of multiple interactions
Scientific Reports, 2022 In this study, mixed-mode chromatography columns have been investigated using multiple analyte interactions. A mixed-mode chromatography column was developed using poly(N-isopropylacrylamide) (PNIPAAm) brush-modified silica beads and poly(3 ...
Kenichi Nagase +2 more
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Fabrication of naphthol-based phenolic polymer coated-silica for mixed-mode chromatography
Journal of Chromatography Open, 2022 This paper introduces a novel phenolic resin-coated silica stationary phase grafted from the surface of amino-functionalized porous silica microspheres through copolymerization of 1,5-dihydroxynaphthalene and 1,3,5-trioxane in the dispersed state ...
Yongxing Hu +2 more
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Data in Brief, 2020 This paper provides the data collected from screening chromatographic resins for their ability to bind and purify recombinant human thioredoxin from Escherichia coli lysate.
Ayswarya Ravi +2 more
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Non-Affinity Purification of Antibodies
Antibodies, 2023 Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale.
Tsutomu Arakawa +7 more
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