Results 51 to 60 of about 4,947,794 (321)

A novel surface-confined glucaminium-based ionic liquid stationary phase for hydrophilic interaction/anion-exchange mixed-mode chromatography

, 2014
Glucaminium-based ionic liquids are a new class of recently developed ionic liquids and prepared by functionalizing the amine group of N-methyl-D-glucamine, which renders them good hydrophilicity due to the presence of the glucose structure and charged ...
Qiao, Lizhen, Li, Hua, 石先哲, 许国旺, Shan, Yuanhong, Shi, Xianzhe, Dou, Abo, Wang, Shuangyuan, Xu, Guowang   +8 more
core   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Mixed-Mode Adsorption of l-Tryptophan on D301 Resin through Hydrophobic Interaction/Ion Exchange/Ion Exclusion: Equilibrium and Kinetics Study

Molecules
The adsorption of l-tryptophan (l-Trp) was studied based on the hydrophobic interaction/ion exchange/ion exclusion mixed-mode adsorption resin D301.
Shengping Wang, Pengfei Jiao, Zhengtian Zhang, Qiuhong Niu   +3 more
doaj   +1 more source

Fases estacionárias modernas para cromatografia líquida de alta eficiência em fase reversa Modern stationary phases for reversed phase high performance liquid chromatography

Química Nova, 2010
This review first discusses the limitations of many of the supports and stationary phases used in reversed phase high performance liquid chromatography and then describes those, developed more recently, that present better stabilities and more versatile ...
Liane Maldaner, Carol H. Collins, Isabel C. S. F. Jardim   +2 more
doaj   +1 more source

Pressure-Flow experiments, packing, and modeling for scale-up of a mixed mode chromatography column for biopharmaceutical manufacturing.

Journal of Chromatography A, 2020
To obtain consistent chromatographic behavior, it is important to develop resin packing methods in accordance with the characteristics of each resin. Resins, particularly those with a significant level of compressibility, require proper knowledge of the ...
J. Prentice, Steven T. Evans, David Robbins, G. Ferreira   +3 more
semanticscholar   +1 more source

Binding and Elution Properties of Mixed-Mode Chromatography and Its Applications for Purification

, 2019
Mixed-mode (multimodal) chromatography now occupies an important place in biopharmaceutical purification. It adds a new dimension to such conventional chromatographies as ion-exchange, hydrophobic interaction, reverse-phase and size-exclusion. Mixed-mode
ARAKAWA, Tsutomu, SANTARELLI, Xavier
core   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source

Probing the Capsid: pH-Driven Gating at the AAV 5-Fold Pore and Its Role in Peptide Ligand Binding

Pharmaceutics
Background/Objectives: Adeno-associated virus (AAV) capsids undergo pH-dependent conformational gating at the 5-fold symmetry pore, but how these structural dynamics shape serotype-specific behavior and affinity-ligand recognition remains unclear ...
Arianna Minzoni, Benjamin Bobay, Shriarjun Shastry, Eduardo Barbieri, Brandon Brino, Crystal Collazo, Shizuo Kamita, Danni Wang, Ciera Khuu, Alexander Polgar, Joseph Siino, Sushmita Koley, Peyton Russelburg, Mark Snyder, Christopher Belisle, Michael Daniele, Stefano Menegatti   +16 more
doaj   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source