Results 51 to 60 of about 4,754,428 (281)

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Probing the Capsid: pH-Driven Gating at the AAV 5-Fold Pore and Its Role in Peptide Ligand Binding

Pharmaceutics
Background/Objectives: Adeno-associated virus (AAV) capsids undergo pH-dependent conformational gating at the 5-fold symmetry pore, but how these structural dynamics shape serotype-specific behavior and affinity-ligand recognition remains unclear ...
Arianna Minzoni, Benjamin Bobay, Shriarjun Shastry, Eduardo Barbieri, Brandon Brino, Crystal Collazo, Shizuo Kamita, Danni Wang, Ciera Khuu, Alexander Polgar, Joseph Siino, Sushmita Koley, Peyton Russelburg, Mark Snyder, Christopher Belisle, Michael Daniele, Stefano Menegatti   +16 more
doaj   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

Binding and Elution Properties of Mixed-Mode Chromatography and Its Applications for Purification

, 2019
Mixed-mode (multimodal) chromatography now occupies an important place in biopharmaceutical purification. It adds a new dimension to such conventional chromatographies as ion-exchange, hydrophobic interaction, reverse-phase and size-exclusion. Mixed-mode
ARAKAWA, Tsutomu, SANTARELLI, Xavier
core   +1 more source

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source

Determination of alpha-solanine in potato by accelerated solvent extraction-hydrophilic interaction chromatography-tandem mass spectrometry

Zhongguo shipin weisheng zazhi, 2020
Objective To establish a method for determination alpha-solanine in potatoes by liquid chromatographytamdem quadrupole mass spectrometry. Methods The potato samples were extracted using the mixed solution of acidity water and organic solvent by ...
Lian WANG, Jiaying CHENG, Dawei CHEN, Xiaohui LI, Hongyan ZENG   +4 more
doaj   +1 more source

Determination of streptomycin and dihydrostreptomycin residues in cheese by weak cation solid phase extraction purification coupled with high performance liquid chromatography-tandem mass spectrometry

Zhongguo shipin weisheng zazhi, 2023
ObjectiveTo develop a determination method for streptomycin and dihydrostreptomycin in cheese using high performance liquid chromatography-tandem mass spectrometry.MethodsThe samples were extracted with 20 mmol/L Na2HPO4 solution （pH 7.4， adjusted by 6 ...
YANG Hao, GONG Pixue, WANG Jun, XUE Xia, WU Hao, WANG Deli   +5 more
doaj   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Separation of polar and nonpolar compounds using HILIC/RPLC mixed-mode chromatography: evaluation of separation performance and retention mechanism of the Acclaim Mixed-Mode HILIC-1 column

Open Chemistry
The Acclaim Mixed-Mode HILIC-1 column, combining hydrophilic interaction (HILIC) and reversed-phase liquid chromatography (RPLC) mechanisms, enables efficient separation of diverse analytes.
Aral Hayriye, Dağ Beşir
doaj   +1 more source

Automated Liquid Handling Extraction and Rapid Quantification of Underivatized Amino Acids and Tryptophan Metabolites from Human Serum and Plasma Using Dual-Column U(H)PLC-MRM-MS and Its Application to Prostate Cancer Study

Metabolites
Amino acids (AAs) and their metabolites are important building blocks, energy sources, and signaling molecules associated with various pathological phenotypes.
Tobias Kipura, Madlen Hotze, Alexa Hofer, Anna-Sophia Egger, Lea E. Timpen, Christiane A. Opitz, Paul A. Townsend, Lee A. Gethings, Kathrin Thedieck, Marcel Kwiatkowski   +9 more
doaj   +1 more source