Results 211 to 220 of about 145,089 (300)

Short foot exercises for flatfoot therapy: Status and prospects. [PDF]

open access: yesSci Prog
Yang H   +7 more
europepmc   +1 more source

Optimal 1TEL–target protein linker character is target protein‐dependent

open access: yesActa Crystallographica Section D, Volume 82, Issue 5, Page 516-532, May 2026.
In this study, we examine the effect of short to medium‐length flexible, semi‐flexible and rigid linkers on the crystallization of a DARPin or the TNK1 UBA domain fused to the 1TEL protein crystallization chaperone, demonstrating that while rigid linkers can impair crystallization and reduce diffraction quality, the ideal linker character remains ...
Maria J. Pedroza Romo   +22 more
wiley   +1 more source

Radiation‐induced peroxide rupture and its temperature‐dependent repair probed by homogeneous X‐ray irradiation

open access: yesActa Crystallographica Section D, Volume 82, Issue 5, Page 492-503, May 2026.
This manuscript investigates the X‐ray‐induced radiolysis of the catalytic C5‐peroxide adduct in urate oxidase crystals, utilizing a top‐hat X‐ray beam to monitor peroxide occupancy across extensive dose series at both 100 K and room temperature. The study reveals a kinetic phase transition where the peroxide decays rapidly via zero‐order kinetics at ...
Symeon Koulas   +4 more
wiley   +1 more source

Radiation damage in sub‐Ångström resolution macromolecular crystallography: a low‐dose study

open access: yesActa Crystallographica Section D, Volume 82, Issue 5, Page 484-491, May 2026.
This first systematic radiation‐damage study at 0.55 Å resolution reveals extensive small conformational changes in response to dose. The findings demonstrate the benefits of using large crystals fully bathed in a large `top‐hat' beam with a uniform fluence profile, together with low‐dose data‐collection protocols, for sub‐Ångström structure ...
Gleb Bourenkov   +7 more
wiley   +1 more source

Mapping the effects of specific radiation damage and solvent radiolysis in buffers and crystals with online UV–Vis absorption spectroscopy

open access: yesActa Crystallographica Section D, Volume 82, Issue 5, Page 504-515, May 2026.
Radiation‐induced changes during X‐ray crystallographic data collection can compromise concurrent spectroscopic studies. Suitable protocols to detect and identify spectral artefacts from crystallization buffers and cryoprotectants are essential for the reliable analysis of chromophoric ligands in macromolecules.Online UV–Vis absorption spectroscopy is ...
Jack Stubbs   +8 more
wiley   +1 more source

Cryo‐EM reveals ArnA contamination during purification of a ciliary protein complex

open access: yesActa Crystallographica Section D, Volume 82, Issue 5, Page 404-410, May 2026.
ArnA contamination was independently observed during the purification of a soluble, low‐yield protein complex, leading to an unintended 3.23 Å resolution cryo‐EM structure. This work broadens recent findings and highlights ArnA as a recurrent His‐tag‐associated contaminant in Escherichia coli purification workflows.Endogenous Escherichia coli proteins ...
Xuguang Jiang, Masahide Kikkawa
wiley   +1 more source

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