Results 21 to 30 of about 3,450 (158)

Multiple-locus variable number tandem repeat analysis for Streptococcus pneumoniae: comparison with PFGE and MLST. [PDF]

PLoS ONE, 2011
In the era of pneumococcal conjugate vaccines, surveillance of pneumococcal disease and carriage remains of utmost importance as important changes may occur in the population.
Karin E M Elberse, Sónia Nunes, Raquel Sá-Leão, Han G J van der Heide, Leo M Schouls   +4 more
doaj   +1 more source

Development and application of MLVA methods as a tool for inter-laboratory surveillance [PDF]

Eurosurveillance, 2013
Multiple-locus variable-number of tandem-repeats analysis (MLVA) has emerged as a valuable method for subtyping bacterial pathogens and has been adopted in many countries as a critical component of their laboratory-based surveillance. Lack of harmonisation and standardisation of the method, however, has made comparison of results generated in different
Nadon, C A, Trees, E, Ng, L K, Møller Nielsen, E, Reimer, A, Maxwell, N, Kubota, K A, Gerner-Smidt, P, Wain, John   +8 more
openaire   +2 more sources

Development of genotyping method of the glanders causative agent based on multiple locus variable-number tandem repeat analysis

Журнал микробиологии, эпидемиологии и иммунобиологии, 2019
The aim was to develop a short MLVA-typing scheme of the causative agent of glanders and to assess the possibility of its use for differentiation of Burkholderia mallei strains and study their genetic polymorphism.Materials and methods.
O. S. Bondareva, G. A. Tkachenko, M. L. Ledeneva, A. A. Baturin, L. V. Lemasova, I. M. Shpak, A. A. Budchenko   +6 more
doaj   +1 more source

MLVA-NET – a standardised web database for bacterial genotyping and surveillance [PDF]

Eurosurveillance, 2008
Strain typing is an important aid to surveillance networks and outbreak investigations of infectious diseases [1]. MLVA (Multilocus VNTR Analysis, with VNTR standing for Variable Number of Tandem Repeats) has emerged as a highly discriminatory and widely applicable genotyping method that is now being applied for strain tracking in a growing number of ...
G, Guigon, J, Cheval, R, Cahuzac, S, Brisse   +3 more
openaire   +2 more sources

Comparing Multiple Locus Variable-Number Tandem Repeat Analyses with Whole-Genome Sequencing as Typing Method for Salmonella Enteritidis Surveillance in The Netherlands, January 2019 to March 2020

Microbiology Spectrum, 2022
In the Netherlands, whole-genome sequencing (WGS) was implemented as routine typing tool for Salmonella Enteritidis isolates in 2019. Multiple locus variable-number tandem repeat analyses (MLVA) was performed in parallel.
Roan Pijnacker, Maaike van den Beld, Kim van der Zwaluw, Anjo Verbruggen, Claudia Coipan, Alejandra Hernandez Segura, Lapo Mughini-Gras, Eelco Franz, Thijs Bosch   +8 more
doaj   +1 more source

Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay [PDF]

BMC Microbiology, 2006
Abstract Background The classification of Brucella into species and biovars relies on phenotypic characteristics and sometimes raises difficulties in the interpretation of the results due to an absence of standardization of the typing reagents. In addition, the resolution of this biotyping is moderate and requires the
Le Fleche, Philippe, Jacques, Isabelle, Grayon, Maggy, Al Dahouk, Sascha, Bouchon, Patrick, Denoeud, France, Nöckler, Karsten, Neubauer, Heinrich, Guilloteau, Laurence, Vergnaud, Gilles   +9 more
openaire   +6 more sources

Proof-of-concept study for successful inter-laboratory comparison of MLVA results [PDF]

Eurosurveillance, 2013
Multiple-locus variable-number of tandem repeats analysis (MLVA) is widely used for typing of pathogens. Methods such as MLVA based on determining DNA fragment size by the use of capillary electrophoresis have an inherent problem as a considerable offset between measured and real (sequenced) lengths is commonly observed.
J T Larsson, M Torpdahl, Collective MLVA working group, E Møller Nielsen   +3 more
openaire   +3 more sources

Investigation of Salmonella Enteritidis outbreaks in South Africa using multi-locus variable-number tandem-repeats analysis, 2013-2015

BMC Infectious Diseases, 2017
Background Salmonella enterica serovar Enteritidis (Salmonella Enteritidis) has become a significant pathogen in South Africa, and the need for improved molecular surveillance of this pathogen has become important.
Munyadziwa Muvhali, Anthony Marius Smith, Andronica Moipone Rakgantso, Karen Helena Keddy   +3 more
doaj   +1 more source

Genetic Characterization of Brucella spp.: Whole Genome Sequencing-Based Approach for the Determination of Multiple Locus Variable Number Tandem Repeat Profiles

Frontiers in Microbiology, 2021
Brucellosis is an important zoonosis that is emerging in some regions of the world, gaining increased relevance with the inclusion of the causing agent Brucella spp. in the class B bioterrorism group.
Ana Pelerito, Alexandra Nunes, Alexandra Nunes, Alexandra Nunes, Teresa Grilo, Joana Isidro, Catarina Silva, Catarina Silva, Ana Cristina Ferreira, Ana Cristina Ferreira, Sylvia Valdezate, Maria Sofia Núncio, Enrico Georgi, João Paulo Gomes, João Paulo Gomes   +14 more
doaj   +1 more source

High throughput MLVA-16 typing for Brucella based on the microfluidics technology [PDF]

BMC Microbiology, 2011
Abstract Background Brucellosis, a zoonosis caused by the genus Brucella, has been eradicated in Northern Europe, Australia, the USA and Canada, but remains endemic in most areas of the world. The strain and biovar typing of Brucella field samples isolated in outbreaks is useful for tracing back source of infection ...
Di Giannatale Elisabetta, Gentile Bernardina, Fillo Silvia, Faggioni Giovanni, Ciammaruconi Andrea, De Santis Riccardo, Ancora Massimo, Lista Florigio   +7 more
openaire   +3 more sources