Antimicrobial agent susceptibilities of Legionella pneumophila MLVA-8 genotypes [PDF]
AbstractLegionella pneumophila causes human lung infections resulting in severe pneumonia. High-resolution genotyping of L. pneumophila isolates can be achieved by multiple-locus variable-number tandem-repeat analysis (MLVA-8). Legionella infections in humans occur as a result of inhalation of bacteria-containing aerosols, thus, our aim was to study ...
Yehonatan Sharaby +5 more
openaire +3 more sources
Development and application of MLVA methods as a tool for inter-laboratory surveillance [PDF]
Multiple-locus variable-number of tandem-repeats analysis (MLVA) has emerged as a valuable method for subtyping bacterial pathogens and has been adopted in many countries as a critical component of their laboratory-based surveillance. Lack of harmonisation and standardisation of the method, however, has made comparison of results generated in different
Nadon, C A +8 more
openaire +2 more sources
Multiple-locus variable number tandem repeat analysis for Streptococcus pneumoniae: comparison with PFGE and MLST. [PDF]
In the era of pneumococcal conjugate vaccines, surveillance of pneumococcal disease and carriage remains of utmost importance as important changes may occur in the population.
Karin E M Elberse +4 more
doaj +1 more source
MLVA-NET – a standardised web database for bacterial genotyping and surveillance [PDF]
Strain typing is an important aid to surveillance networks and outbreak investigations of infectious diseases [1]. MLVA (Multilocus VNTR Analysis, with VNTR standing for Variable Number of Tandem Repeats) has emerged as a highly discriminatory and widely applicable genotyping method that is now being applied for strain tracking in a growing number of ...
G, Guigon +3 more
openaire +2 more sources
Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay [PDF]
Abstract Background The classification of Brucella into species and biovars relies on phenotypic characteristics and sometimes raises difficulties in the interpretation of the results due to an absence of standardization of the typing reagents. In addition, the resolution of this biotyping is moderate and requires the
Le Fleche, Philippe +9 more
openaire +6 more sources
Human infections with Brucella melitensis are occasionally reported in Sweden, despite the fact that the national flocks of sheep and goats are officially free from brucellosis. The aim of our study was to analyze 103 isolates of B.
Lorena Sacchini +6 more
doaj +1 more source
Prevalence and genetic distribution of Legionella spp. in public bath facilities in Kobe City, Japan
Legionella is an important waterborne pathogen that causes legionellosis. Public baths are considered the primary cause of legionellosis infection in Japan. We investigated the prevalence and genetic distribution of 338 Legionella spp.
Shoko Komatsu +2 more
doaj +1 more source
Proof-of-concept study for successful inter-laboratory comparison of MLVA results [PDF]
Multiple-locus variable-number of tandem repeats analysis (MLVA) is widely used for typing of pathogens. Methods such as MLVA based on determining DNA fragment size by the use of capillary electrophoresis have an inherent problem as a considerable offset between measured and real (sequenced) lengths is commonly observed.
J T Larsson +3 more
openaire +3 more sources
New genetic markers for Burkholderia pseudomallei strains typing
Burkholderia pseudomallei is the causative agent of melioidosis, a serious infectious disease in humans and animals that may be asymptomatic long-term and quickly develop to pneumonia and septicemia in immunocompromised state and due to predisposing ...
Margarita L. Ledenyova +2 more
doaj +1 more source
High throughput MLVA-16 typing for Brucella based on the microfluidics technology [PDF]
Abstract Background Brucellosis, a zoonosis caused by the genus Brucella, has been eradicated in Northern Europe, Australia, the USA and Canada, but remains endemic in most areas of the world. The strain and biovar typing of Brucella field samples isolated in outbreaks is useful for tracing back source of infection ...
Di Giannatale Elisabetta +7 more
openaire +3 more sources

