Expression of an innate immune element (mouse hepcidin-1) in baculovirus expression system and the comparison of its function with synthetic human hepcidin-25 [PDF]
Hepcidin is an innate immune element which decreases the iron absorption from diet and iron releasing from macrophage cell. In contrast to the chemical iron chelators, there has been limited effort applied to the specific use of hepcidin as a new drug ...
Alimohammadian, M.+5 more
core +1 more source
Molecular, Enzymatic, and Cellular Characterization of Soluble Adenylyl Cyclase From Aquatic Animals. [PDF]
The enzyme soluble adenylyl cyclase (sAC) is the most recently identified source of the messenger molecule cyclic adenosine monophosphate. sAC is evolutionarily conserved from cyanobacteria to human, is directly stimulated by [Formula: see text] ions ...
Acin-Perez+58 more
core +2 more sources
Molecular cloning of duck CD40 and its immune function research
: Cosignal molecules are cell surface molecules that transduce signals to other cells to modulate immune response positively (costimulate) or negatively (cosuppress).
Rujuan Zhang+19 more
doaj
Cloning and sequence analysis of cDNAs encoding the cytosolic precursors of subunits GapA and GapB of chloroplast glyceraldehyde-3-phosphate dehydrogenase from pea and spinach [PDF]
Chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is composed of two different subunits, GapA and GapB. cDNA clones containing the entire coding sequences of the cytosolic precursors for GapA from pea and for GapB from pea and spinach have ...
F Sanger+35 more
core +1 more source
Plasmid ColE1 as a Molecular Vehicle for Cloning and Amplification of DNA [PDF]
Vickers Hershfield+4 more
openalex +1 more source
Characterization of an Escherichia coli plasmid encoding for synthesis of heat-labile toxin: molecular cloning of the toxin determinant [PDF]
Magdalene So, W S Dallas, Stanley Falkow
openalex +1 more source
Medicago truncatula functional genomics: an invaluable resource for studies on agriculture sustainability [PDF]
Legume functional genomics has moved many steps forward in the last two decades thanks to the improvement of genomics technologies and to the efforts of the research community.
Calderini, Ornella+2 more
core
Efficient cloning of linear DNA inserts (ECOLI) into plasmids using site-directed mutagenesis
This study introduces a novel cost-effective technique for cloning of linear DNA plasmid inserts, aiming to address the associated expenses linked with popular in vitro DNA assembly methods.
Petra Paclikova, Jakub Harnos
doaj +1 more source
Molecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI. [PDF]
Dean H. Hamer, C A Thomas
openalex +1 more source
Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3 [PDF]
Large-scale production of plasmid DNA to prepare therapeutic gene vectors or DNA-based vaccines requires a suitable bacterial host, which can stably maintain the plasmid DNA during industrial cultivation.
Al-Allaf, FA+4 more
core +1 more source