Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
Molecular cloning of PRD-like homeobox genes expressed in bovine oocytes and early IVF embryos. [PDF]
Yaşar B+12 more
europepmc +1 more source
Molecular cloning of chicken aggrecan [PDF]
L Chandrasekaran, M L Tanzer
openaire +2 more sources
Molecular cloning of a human histocompatibility antigen cDNA fragment.
Hidde L. Ploegh+2 more
openalex +1 more source
Molecular cloning of African swine fever virus DNA [PDF]
Victoria Ley+5 more
openalex +1 more source
UDP‐glucose dehydrogenase variants cause dystroglycanopathy
Abstract UDP‐glucose dehydrogenase (UGDH) variants have been associated with hypotonia, developmental delay, and epilepsy. We report the first pathologic evidence of dystroglycanopathy in siblings with UGDH variants. Both presented around 6 months with developmental delay and elevated creatinine kinase.
Anna M. Reelfs+8 more
wiley +1 more source
Molecular cloning and characterization of a brassinosteriod biosynthesis-related gene PtoCYP90D1 from Populus tomentosa. [PDF]
Song J+5 more
europepmc +1 more source
Molecular cloning and functional characterization of BcTSA in the biosynthesis of indole alkaloids in Baphicacanthus cusia. [PDF]
Guo Z+6 more
europepmc +1 more source
Molecular cloning of copper resistance genes from Pseudomonas syringae pv. tomato
Carol L. Bender, Donald A. Cooksey
openalex +1 more source
Molecular cloning of the ferrichrome-iron receptor of Escherichia coli K-12 [PDF]
James W. Coulton+2 more
openalex +1 more source