Results 231 to 240 of about 5,043,369 (304)

Molecular cloning

Fish & Shellfish Immunology, 2020
Transcription factor c-Jun is a member of AP-1 transcription complex that can be induced by various pathogens and plays a broad regulatory role in vertebrate immune response. In this study, the complete c-Jun cDNA of large yellow croaker Larimichthys crocea (Lcc-Jun) was cloned, whose open reading frame (ORF) is 984 bp long and encodes a protein of 327
Libing Xu   +4 more
semanticscholar   +4 more sources

Molecular cloning.

Methods in Enzymology, 2013
This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
J. Lessard
semanticscholar   +4 more sources

Molecular Cloning of Ricin

2020
A variety of strategies have been used to obtain cDNA and genomic clones encoding ricin. Since their isolation these sequences have been manipulated to allow expression of A chain (19) and A chain mutants (15,20,34), B chain (14,21-23) and proricin (24).
L M, Roberts, J W, Tregear, J M, Lord
openaire   +2 more sources

Molecular cloning of human RP105

European Journal of Immunology, 1997
AbstractRP105 is a 105‐kDa type I membrane protein of the leucine‐rich repeat (LRR) family. Anti‐RP105 sensitizes B cells to antigen‐receptor‐mediated apoptosis, but protects B cells from radiation‐induced apoptosis and stimulates B cell proliferation. The sequence of the mouse RP105 has been reported.
Odette de Bouteiller   +9 more
openaire   +3 more sources

Molecular cloning of the cDNA for ligatin

Journal of Cell Science, 1989
ABSTRACT We describe the first isolation and sequence of a partial cDNA clone encoding ligatin, a trafficking receptor for phosphoglycoproteins. The clone was isolated from a human U937 promonocyte λgtll cDNA library using rabbit antiserum to rat ileal ligatin.
Ye-Shih Ho   +3 more
openaire   +3 more sources

Molecular Cloning of PCR Products

Current Protocols in Molecular Biology, 2001
AbstractIt is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain high‐quality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified
Ayoub Rashtchian   +2 more
openaire   +3 more sources

Molecular Cloning in the Streptococci

1982
The genus Streptococcus contains a large number of species able to cause infection in humans and in animals. Our research efforts have revolved around the study of streptococci that normally reside in the human oral cavity. In this regard, Streptococcus mutans is thought to play an important role in the etiology of dental caries (tooth decay) in humans
Francis L. Macrina   +3 more
openaire   +3 more sources

Molecular Cloning of Steroid Hydroxylases

Endocrine Research, 1984
Recombinant plasmids specific for bovine adrenocortical cytochromes P-450scc and P-45011 beta have been identified and characterized. Using these cDNA inserts as probes, it is found that tissue specificity of gene expression for these two proteins is as expected. Cytochrome P-450scc mRNA is found in adrenocortical and corpus luteum RNA while cytochrome
Evan R. Simpson   +3 more
openaire   +3 more sources

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