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[26] Molecular cloning of laminin
1987Publisher Summary Molecular cloning can be used to answer questions about the domain structure of laminin, its relationship to other matrix glycoproteins, the subunit composition of the laminin synthesized by different cell types, the possible existence of alternate forms of laminin polypeptides, the structure and chromosomal localization of laminin ...
Barlow, DP, McVey, JH, Hogan, BLM
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Molecular cloning of feline CD34
Veterinary Immunology and Immunopathology, 2003In humans, baboons, dogs and mice CD34 is a cell surface molecule that is expressed on primitive hematopoietic cells and in all these species CD34 positive cells can be used to effect long-term haematopoietic reconstitution. CD34 positive haematopoietic cells therefore provide a convenient and relatively small cell population to target when attempting ...
Gouri Yogalingam+2 more
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Molecular cloning in heterologous systems
1983During the last years the knowledge accumulated by fundamental research of DNA recombination in vitro became more relevant to biotechnology.
Christine Dr. Lang-Hinrichs, Karl Esser
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Molecular Cloning of Human Amidophosphoribosyltransferase
Biochemical and Biophysical Research Communications, 1993The cDNA of human amidophosphoribosyltransferase (EC 2.4.2.14, ATase), which is the supposed regulatory allosteric enzyme of de novo purine nucleotide biosynthesis, has been cloned from human hepatoma (HepG2) cDNA library. The predicted open reading frame encodes a protein of 517 amino acids with a deduced molecular weight (Mr) of 57,398, which is ...
H, Iwahana+7 more
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Molecular Cloning of the Thyrotropin Receptor
Science, 1989The pituitary hormone thyrotropin, or thyroid-stimulating hormone (TSH), is the main physiological agent that regulates the thyroid gland. The thyrotropin receptor (TSHR) was cloned by selective amplification with the polymerase chain reaction of DNA segments presenting sequence similarity with genes for G protein-coupled receptors. Out of
Parmentier, Marc+8 more
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Molecular Cloning of HumanFrizzled-6,
Biochemical and Biophysical Research Communications, 1998The Frizzled genes encode receptors for WNTs, secreted glycoproteins implicated in development as well as in carcinogenesis. In this paper, we report molecular cloning of Hfz6, the human homologue of Mfz6. Nucleotide sequence analysis showed that the Hfz6 gene encodes the 706 amino-acid protein with seven transmembrane domains, a cystein-rich domain in
Masaru Katoh+4 more
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Isolation and molecular cloning of a fish myeloperoxidase
Molecular Immunology, 2008Myeloperoxidase (MPO) is a conspicuous enzyme in neutrophils of many fish species. Although the MPO gene has been identified in some fish species, the structure and functions of the protein remain to be determined in these vertebrates. In the present study, we isolated turbot neutrophil MPO from kidney cells by affinity chromatography, with Ulva rigida
Castro, Rosario+4 more
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Molecular cloning of the interleukin-1 beta converting enzyme.
Science, 1992Interleukin-1 beta (IL-1 beta) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor.
D. Cerretti+9 more
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Molecular cloning and expression of internalin in Listeria
1994Publisher Summary This chapter discusses the molecular cloning and expression of internalin in Listeria . Listeria monocytogenes is a ubiquitous gram-positive bacterium that causes severe infections in humans and in a variety of warm-blooded animals. This pathogen has emerged as an important agent of foodborne disease in the last decade.
Gaillard, Jean-Louis+3 more
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Molecular cloning of mouse allantoicase cDNA
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 2001The uric acid degradation pathway is progressively lost during vertebrate evolution. In mammals, the end product of this catabolic pathway is allantoin and, therefore, no allantoicase should be present in mouse tissues. Surprisingly, we have found an expressed sequence tag (EST) from mouse testis with high similarity to allantoicase.
VIGETTI, DAVIDE+2 more
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