Results 21 to 30 of about 261,173 (297)

Advances of multiplex ligation-dependent probe amplification technology in molecular diagnostics

BioTechniques, 2022
Multiplex ligation-dependent probe amplification (MLPA) is a multiplex copy number analysis tool which is routinely used to detect large mutations in genetic diseases.
Xiaoni Fu, Yinmin Shi, Jiying Ma, Kaiqian Zhang, Guowei Wang, Gang Li, Lei Xiao, Huijuan Wang   +7 more
doaj   +1 more source

Chemical Biology of Autophagy-Related Proteins With Posttranslational Modifications: From Chemical Synthesis to Biological Applications

Frontiers in Chemistry, 2020
Macroautophagy (hereafter referred to as autophagy) is an evolutionarily conserved lysosomal degradation pathway in all eukaryotic cells, which is critical for maintaining cell homeostasis. A series of autophagy-related (ATG) proteins are involved in the
Yu Luo, Chen Jiang, Lihua Yu, Aimin Yang
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Molecular conformations and dynamics in the extracellular matrix of mammalian structural tissues: Solid-state NMR spectroscopy approaches

Matrix Biology Plus, 2021
Solid-state NMR spectroscopy has played an important role in multidisciplinary studies of the extracellular matrix. Here we review how solid-state NMR has been used to probe collagen molecular conformations, dynamics, post-translational modifications and
Adrian Murgoci, Melinda Duer
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Multiscale Structural Elucidation of Peptide Nanotubes by X-Ray Scattering Methods

Frontiers in Bioengineering and Biotechnology, 2021
This mini-review presents the structural investigations of the self-assembled peptide nanotubes using X-ray scattering techniques. As compared to electron microscopy, scattering methods enable studies of nanotubes in solution under the appropriate ...
Theyencheri Narayanan, Axel Rüter, Ulf Olsson   +2 more
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Multiplex ligation-dependent probe amplification – a short overview

Romanian Journal of Laboratory Medicine, 2020
Multiplex Ligation-dependent Probe Amplification is a technique proposed for the detection of deletions or duplications that may lead to copy number variations in genomic DNA, mainly due to its higher resolution, and shorter overall diagnosis time, when ...
Moldovan Valeriu, Moldovan Elena
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Avaliação de uma sonda genética (Sistema Accuprobe, Gen Probe®) para identificação de organismos do complexo Mycobacterium tuberculosis, em comparação com métodos tradicionais de caracterização Evaluation of a genetic probe (Gen-Probe Accuprobe® system) in comparison to traditional methods for identifying members of the Mycobacterium tuberculosis complex

Jornal Brasileiro de Pneumologia, 2005
INTRODUÇÃO: O aparecimento da co-infecção tuberculose/síndrome da imunodeficiência adquirida, o aumento de doenças por micobactérias não tuberculosas e as confusões que estas podem ter com as emergentes cepas multirresistentes exigem respostas ...
Delurce Tadeu de Araujo Spada, Manoel Armando Azevedo dos Santos, Elisabete A. Almeida, Marcos Augusto, Maria Idemar Pedrosa Albarral, Fernando Augusto Fiuza de Melo   +5 more
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Discrepancies over the onset of surfactant monomer aggregation interpreted by fluorescence, conductivity and surface tension methods

Química Nova, 1998
Molecular probe techniques have made important contributions to the determination of microstructure of surfactant assemblies such as size, stability, micropolarity and conformation.
Maria de Fátima Carvalho Costa, Sandro José Froehner, Ângelo Adolfo Ruzza, Sônia de Fátima Santos, Dino Zanette   +4 more
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Optical force microscopy: combining light with atomic force microscopy for nanomaterial identification

Nanophotonics, 2019
Scanning probe techniques have evolved significantly in recent years to detect surface morphology of materials down to subnanometer resolution, but without revealing spectroscopic information.
Jahan Nusrat, Wang Hanwei, Zhao Shensheng, Dutta Arkajit, Huang Hsuan-Kai, Zhao Yang, Chen Yun-Sheng   +6 more
doaj   +1 more source

Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning

BMC Biotechnology, 2019
Background Long Adapter Single-Stranded Oligonucleotide (LASSO) probes were developed as a novel tool for massively parallel cloning of kilobase-long genomic DNA sequences. LASSO dramatically improves the capture length limit of current DNA padlock probe
Syukri Shukor, Alfred Tamayo, Lorenzo Tosi, H. Benjamin Larman, Biju Parekkadan   +4 more
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Biomembrane force probe (BFP): Design, advancements, and recent applications to live‐cell mechanobiology

Exploration, 2023
Mechanical forces play a vital role in biological processes at molecular and cellular levels, significantly impacting various diseases such as cancer, cardiovascular disease, and COVID‐19.
Laura Moldovan, Caroline Haoran Song, Yiyao Catherine Chen, Haoqing Jerry Wang, Lining Arnold Ju   +4 more
doaj   +1 more source