Results 91 to 100 of about 631,116 (314)

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Molecular assay optimized by Taguchi experimental design method for venous thromboembolism investigation

, 2011
Two mutations - Factor V Leiden (1691G > A) and the 20210G > A on the Prothrombin gene - are key risk factors for a frequent and potentially fatal disorder called Venous Thromboembolism.
Pontes, Fabricio J. [UNESP], Alberto, Fernando Lopes, Ubirajara R. Ferreira, Messias Borges Silva, Moyses, Cinthia B., Celani de Souza, Helder Jose [UNESP], Sanches da Silva, Carlos Eduardo, Cinthia B. Moyses, Fabrício J. Pontes, Ferreira, Ubirajara R. [UNESP], Silva, Messias Borges, Celani de Souza, Helder Jose, Helder Jose Celani de Souza, Carlos Eduardo Sanches da Silva, Fernando Lopes Alberto, Roberto N. Duarte, Silva, Messias Borges [UNESP], Ferreira, Ubirajara R., Pontes, Fabricio J., Duarte, Roberto N.   +19 more
core   +1 more source

Nonenzymatic synthesis of anomerically pure, mannosyl-based molecular probes for scramblase identification studies [PDF]

, 2020
The chemical synthesis of molecular probes to identify and study membrane proteins involved in the biological pathway of protein glycosylation is described.
Probst, Markus, Bütikofer, Peter, Picca, Giovanni, Langenegger, Simon M., Khorev, Oleg, Menon, Anant K, Häner, Robert   +6 more
core   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Molecular identification of blaSHV, blaLEN and blaOKP β-lactamase genes in Klebsiella pneumoniae by bi-directional sequencing of universal SP6- and T7-sequence-tagged blaSHV-PCR amplicons

, 2009
Plasmid encoded blaSHV enzymes represent an important sub-group of class A β-lactamases causing an ESBL-phenotype which is increasingly found in Enterobacteriaceae including Klebsiella pneumoniae.
Tärnberg, Maria,, Nilsson, Lennart E.,, Nilsson, Lennart E., Monstein, Hans-Jürg, Monstein, Hans-Jürg,, Tärnberg, Maria   +5 more
core   +1 more source

A reversible photochromic covalent organic framework

Nature Communications
Covalent organic frameworks are a type of crystalline porous materials that linked through covalent bond, and they have numerous potential applications in adsorption, separation, catalysis, and more.
Xue-Tian Li, Meng-Jing Li, Yuan-Liang Tian, Shu-Lin Han, Lei Cai, Hui-Chao Ma, Ying-Qiang Zhao, Gong-Jun Chen, Yu-Bin Dong   +8 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Versatile Dicyanomethylene-Based Fluorescent Probes for the Detection of β-Amyloid in Alzheimer’s Disease: A Theoretical Perspective

, 2022
Motivated by the growing demand for target chemosensors designed with diagnostic or therapeutic capability for fibrils related to amyloidosis diseases, we investigated in the present work the response mechanism of dicyanomethylene-based fluorescent ...
Leng, Jiancai, Jiancai Leng, Wei Hu, Zhang, Yujin, Zhang, Mengna, Hu, Wei, Haoqing Fu, Fu, Haoqing, Mengna Zhang, Yujin Zhang   +9 more
core   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Multicomponent one-pot construction of benzo[f]quinoline-linked covalent organic frameworks for H2O2 photosynthesis

Nature Communications
The exploration of stable and functional linkages by multicomponent reactions to enrich the stability and diversity of covalent organic frameworks (COFs) and to broaden their potential applications is of fundamental significance to the development of ...
Ke-Hui Xie, Guang-Bo Wang, Fang Huang, Fei Zhao, Jing-Lan Kan, Zi-Zheng Chen, Lei Cai, Shu-Lin Han, Yan Geng, Yu-Bin Dong   +9 more
doaj   +1 more source