Results 91 to 100 of about 104,482 (285)

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

A modular artificial intelligence framework to facilitate fluorophore design

Nature Communications
Fluorescence imaging, indispensable for fundamental research and clinical practice, has been driven by advances in fluorophores. Despite fast growth over the years, many available fluorophores suffer from insufficient performances, and their development ...
Yuchen Zhu, Jiebin Fang, Shadi Ali Hassen Ahmed, Tao Zhang, Su Zeng, Jia-Yu Liao, Zhongjun Ma, Linghui Qian   +7 more
doaj   +1 more source

Apparatus and method for characterizing the transmission efficiency of a mass spectrometer [PDF]

, 1990
An electron/ion coincidence technique is employed to characterize the absolute mass dependent transmission efficiency of mass spectrometers. The technique is not dependent upon the partial pressure of the sample beam or the ionization cross sections of ...
Srivasytava, Santash
core   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

A rank inequality for the annular Khovanov homology of 2-periodic links

, 2017
For a 2-periodic link $\tilde L$ in the thickened annulus and its quotient link $L$, we exhibit a spectral sequence with $E^1 \cong AKh(\tilde L) \otimes_{\mathbb{F}_2} \mathbb{F}_2[\theta, \theta^{-1}] \rightrightarrows E^\infty \cong AKh(L) \otimes_ ...
Zhang, Melissa
core   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Lab-on-Chip for Testing Myelotoxic Effect of Drugs and Chemicals [PDF]

, 2014
This paper was presented at the 4th Micro and Nano Flows Conference (MNF2014), which was held at University College, London, UK. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, IPEM, the ...
4th Micro and Nano Flows Conference (MNF2014), Bonomi, A, Cavicchini, L, Cocce, V, Fiore, GB, Gazaneo, A, Occhetta, P, Pessina, A, Rasponi, M, Redaelli, A   +9 more
core  

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

NIR-activatable nitric oxide generator based on nanoparticles loaded small-molecule photosensitizers for synergetic photodynamic/gas therapy

Journal of Nanobiotechnology
Background Therapeutic approaches that combine conventional photodynamic therapy (PDT) with gas therapy (GT) to sensitize PDT are an attractive strategy, but the molecular structure design of the complex lacks effective guiding strategies. Results Herein,
Lili Fu, Yan Huang, Xin Shan, Xiao Sun, Xinlei Wang, Xiaoyan Wang, Lingxin Chen, Shui Yu   +7 more
doaj   +1 more source