Distinct filament morphology and membrane tethering features of the dual FtsZ paralogs in Odinarchaeota. [PDF]
Kumari J +12 more
europepmc +1 more source
Structural investigations connect the disordered N-terminal extension of HypB to the activities of HypB and SlyD in E. coli. [PDF]
Law WWH +4 more
europepmc +1 more source
Phylogenetic and biochemical analysis of the evolution and interdomain modularity of bifunctional L-fucokinase/GDP-fucose pyrophosphorylases. [PDF]
Smith NT +5 more
europepmc +1 more source
Structural snapshots capture nucleotide release at the μ-opioid receptor. [PDF]
Khan S +6 more
europepmc +1 more source
Various methods to detect small GTPase activation: from radioisotope-based methods to the Small GTPase ActIvitY ANalysing (SAIYAN) system. [PDF]
Maeda M, Saito K.
europepmc +1 more source
Intracellular Transport of Monomeric Peptides, (Poly)Peptide-Based Coacervates and Fibrils: Mechanisms and Prospects for Drug Delivery. [PDF]
Vedekhina T +4 more
europepmc +1 more source
Crystal structure of the class V GTP-binding RNA aptamer bound to its ligand: GTP recognition by a topologically complex intermolecular G-quadruplex. [PDF]
Stafflinger H +10 more
europepmc +1 more source
Structure and function of the RNA polymerase complex of Borna disease virus, a nuclear-replicating non-segmented negative-strand RNA virus. [PDF]
Gibbs E +7 more
europepmc +1 more source
A cDNA Cloning Method for Monomeric GTP-Binding Proteins by Ligand Blotting
We have developed a convenient method of cDNA cloning for monomeric GTP-binding proteins. The method consists of a serial dilution of cDNA expression library, a separation of proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and a ligand blotting; each group containing the proteins from about 1000 independent colonies ...
Yukio Nagano, Ryuichi Matsuno, Y. Sasaki
openalex +3 more sources

