Results 131 to 140 of about 28,236 (160)
Molecular analysis of the monomeric GTP-binding proteins of oligodendrocytes
Molecular Brain Research, 1997 Vesicle transport plays an important role in the formation of myelin. Transport of proteins, including proteolipid protein and myelin associated glycoprotein, from their site of synthesis in the endoplasmic reticulum in the perikaryon of the oligodendrocytes, to myelin, takes place via carrier vesicles. The mechanisms that regulate vesicle transport in
Jorge N. Larocca +4 more
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An Expression Cloning Method to Identify Monomeric GTP-Binding Proteins by GTP Overlay
Analytical Biochemistry, 1997 We have developed a method for identifying monomeric GTP-binding proteins that is based on probing plasmid expression libraries with [alpha-32P]GTP. The method involves the production of nitrocellulose replica filter lifts from a plasmid cDNA expression library and treatment of the filters with chloroform vapor to lyse the Escherichia coli and to ...
Keiko Kadono-Okuda, Douglas A. Andres
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A cDNA Cloning Method for Monomeric GTP-Binding Proteins by Ligand Blotting
Analytical Biochemistry, 1993 We have developed a convenient method of cDNA cloning for monomeric GTP-binding proteins. The method consists of a serial dilution of cDNA expression library, a separation of proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and a ligand blotting; each group containing the proteins from about 1000 independent colonies ...
Yukiko Sasaki, Y. Nagano, R. Matsuno
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Expression cloning to identify monomeric GTP-binding proteins by GTP overlay
, 2001 Publisher Summary More than 70 small Ras-related GTP-binding proteins have been identified and additional members of the family continue to be discovered. The majority of these proteins have been isolated by screening genomic or cDNA libraries at low stringency with oligonucleotide mixes corresponding to the conserved guanine nucleotide-binding ...
Douglas Andres
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Russian Journal of Plant Physiology, 2007 The control of plant growth, differentiation, and development is considered in relation to the involvement of monomeric GTP-binding proteins (mG-proteins) in the extra-and intracellular signal transduction. The principal attention is paid to Rop mG-proteins, unique small GTPases of eukaryotic cells functioning during various developmental stages of ...
I. E. Moshkov, G. V. Novikova
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Plant Science, 1999 Abstract Differential display of mRNAs from embryos of Avena fatua L. caryopses was used to isolate an mRNA more abundant in nondormant than dormant caryopses during early imbibition. The DNA sequence of the corresponding 579 bp cDNA, termed Af SAR1, is 92% identical to an Arabidopsis cDNA encoding the monomeric GTP-binding protein sar1p.
Harwood J. Cranston +3 more
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Russian Journal of Plant Physiology, 2008 In plants, Rab proteins represent the largest family of monomeric GTP-binding proteins (mG-proteins). As distinct from animal cells comprising 40 subfamilies of Rab proteins, which are the key regulators of intracellular vesicular transport, numerous Rab proteins in Arabidopsis and other plant species could be grouped in only eight subfamilies on the ...
G. V. Novikova, I. E. Moshkov
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, 2002 Ernst Helmreich
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Monomeric
, 2003 Ernst Helmreich
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