Results 61 to 70 of about 685,288 (265)

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Short-term effects of foot surgery on walking-related pain, function, and satisfaction in patients with Charcot–Marie–Tooth disease: a prospective cohort study

Frontiers in Neurology
IntroductionPatients with Charcot–Marie–Tooth disease (CMT) often suffer from walking-related pain (WRP), muscle weakness, foot deformities, and reduced ankle dorsiflexion (DF), which affects their ability to walk and daily activities. Functional surgery
Giacomo Basini, Chiara Rambelli, Chiara Rambelli, Martina Galletti, Paolo Zerbinati, Paolo Zerbinati, Paolo Prati, Francesca Mascioli, Stefano Masiero, Davide Mazzoli, Andrea Merlo   +10 more
doaj   +1 more source

Phosphoinositides and inositol phosphates as molecular glues

FEBS Letters, EarlyView.
Inositol phosphates (IPs) and phosphoinositides (PIPs) regulate diverse eukaryotic processes. Beyond recruiting signaling proteins or acting as structural cofactors, recent studies suggest they mediate protein–protein interactions as natural molecular glues.
Aleshia Seaton‐Terry, Alexander J. Cutright, Mong Na Claire Loi, Jessica Martin, Joseph W. Gilligan, Jakub N. Kubina, Michael J. Arambula, Susanna Tanada Palmu, Daniella Zhou, Raymond D. Blind   +9 more
wiley   +1 more source

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas F. Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

Exoskeleton therapy in cerebral palsy: improved gait endurance without kinematic change

Frontiers in Human Neuroscience
IntroductionCerebral palsy (CP) often leads to impairments in movement and posture, limiting functional mobility. Robotic-assisted gait training (RAGT) using powered exoskeletons has emerged as a novel approach to enhance gait in individuals with CP ...
Paweł Chmara, Sabina Brazevic, Marek Jóźwiak, Brian Po-Jung Chen, Faustyna Manikowska   +4 more
doaj   +1 more source

Report on instrumented gait assessment in patients with spinal muscular atrophy: a prospective follow-up (2024–2026) [PDF]

Chirurgia Narządów Ruchu i Ortopedia Polska
Introduction. Gait disorders are a key determinant of functional capacity in ambulatory patients with spinal muscular atrophy (SMA), significantly affecting quality of life and fall risk. Three-dimensional clinical gait analysis (3DCGA) remains
Sabina Brazevic, Amadeusz Brzykcy, Paweł Chmara, Radosław Rutkowski, Marek Jóźwiak   +4 more
doaj   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Short-term reduction of ankle spasticity after surgical lengthening of the triceps surae in chronic post-stroke patients: a retrospective cohort study

Frontiers in Neurology
IntroductionIn post-stroke patients with equinovarus foot deformity (EVFD), soft tissue rearrangements may contribute to muscle overactivity when a muscle is stretched or tension is applied.
Martina Galletti, Davide Mazzoli, Paolo Zerbinati, Paolo Zerbinati, Chiara Rambelli, Chiara Rambelli, Giacomo Basini, Paolo Prati, Francesca Mascioli, Stefano Masiero, Andrea Merlo   +10 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source