Genes & Development, 2000 The activities of several mRNA processing factors are coupled to transcription through binding to RNA polymerase II (Pol II). The largest subunit of Pol II contains a repetitive carboxy-terminal domain (CTD) that becomes highly phosphorylated during ...
P. Komarnitsky, E. Cho, S. Buratowski
semanticscholar +1 more source
Fip1 is a multivalent interaction scaffold for processing factors in human mRNA 3′ end biogenesis
eLife, 2022 3′ end formation of most eukaryotic mRNAs is dependent on the assembly of a ~1.5 MDa multiprotein complex, that catalyzes the coupled reaction of pre-mRNA cleavage and polyadenylation. In mammals, the cleavage and polyadenylation specificity factor (CPSF)
Lena Maria Muckenfuss +4 more
doaj +1 more source
The Histone 3'-Terminal Stem-Loop-Binding Protein Enhances Translation through a Functional and Physical Interaction with Eukaryotic Initiation Factor 4G (eIF4G) and eIF3 [PDF]
, 2002 Metazoan cell cycle-regulated histone mRNAs are unique cellular mRNAs in that they terminate in a highly conserved stem-loop structure instead of a poly(A) tail.
Gallie, Daniel R +4 more
core +4 more sources
A quantitative comparison of sRNA-based and protein-based gene regulation [PDF]
, 2008 Small, non-coding RNAs (sRNAs) play important roles as genetic regulators in prokaryotes. sRNAs act post-transcriptionally via complementary pairing with target mRNAs to regulate protein expression.
Berg HC +5 more
core +3 more sources
PLoS Genetics, 2017 As model organism-based research shifts from forward to reverse genetics approaches, largely due to the ease of genome editing technology, a low frequency of abnormal phenotypes is being observed in lines with mutations predicted to lead to deleterious ...
Jennifer L. Anderson +8 more
semanticscholar +1 more source
Stem–loop RNA labeling can affect nuclear and cytoplasmic mRNA processing
RNA: A publication of the RNA Society, 2017 The binding of sequence-specific RNA-interacting proteins, such as the bacteriophage MS2 or PP7 coat proteins, to their corresponding target sequences has been extremely useful and widely used to visualize single mRNAs in vivo.
Stephanie Heinrich +3 more
semanticscholar +1 more source
Notch mRNA expression in Drosophila embryos is negatively regulated at the level of mRNA 3' processing. [PDF]
PLoS ONE, 2009 Notch receptor regulates differentiation of almost all tissues and organs during animal development. Many mechanisms function at the protein level to finely regulate Notch activity.
Andrew K Shepherd +2 more
doaj +1 more source
RNA: A publication of the RNA Society, 2018 By using a cell fraction technique that separates chromatin-associated nascent RNA, newly completed nucleoplasmic mRNA and cytoplasmic mRNA, we have shown in a previous study that residues in exons are methylated (m6A) in nascent pre-mRNA and remain ...
R. Darnell, Shengdong Ke, J. Darnell
semanticscholar +1 more source
A High-Throughput Method for Illumina RNA-Seq Library Preparation. [PDF]
, 2012 With the introduction of cost effective, rapid, and superior quality next generation sequencing techniques, gene expression analysis has become viable for labs conducting small projects as well as large-scale gene expression analysis experiments. However,
Daniel H Chitwood +8 more
core +6 more sources
Loss of the histone pre-mRNA processing factor stem-loop binding protein in Drosophila causes genomic instability and impaired cellular proliferation. [PDF]
PLoS ONE, 2009 BACKGROUND:Metazoan replication-dependent histone mRNAs terminate in a conserved stem-loop structure rather than a polyA tail. Formation of this unique mRNA 3' end requires Stem-loop Binding Protein (SLBP), which directly binds histone pre-mRNA and ...
Harmony R Salzler +3 more
doaj +1 more source