Results 31 to 40 of about 1,219 (173)

A simple and rapid agarose gel electrophoresis method to assess CpG methylation of DNA

BioTechniques
The presence of 5-methylcytosine at CpG sites in mammalian DNA plays a significant role in various biological processes, both normal and aberrant. Similarly, CpG site methylation status of therapeutic DNAs can affect activity and therapeutic efficacy, as
Marco Paliza-Carre, Ge Chen, Shahrokh Shabahang   +2 more
doaj   +1 more source

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

Symbolic Regression and Multi‐Objective Optimization of the Flory–Huggins Interaction Parameter for Hydrogels

Advanced Engineering Materials, EarlyView.
We develop a data‐driven method to derive the mathematical expressions of the Flory–Huggins interaction parameter χ for the swelling behavior of temperature–responsive hydrogels. Starting from initial assumptions of χ, our workflow combines Bayesian optimization, Flory–Rehner theory, and symbolic regression to generate candidate χ expressions.
Yawen Wang, Adrian Ehrenhofer, Thomas Wallmersperger   +2 more
wiley   +1 more source

Forecast U.S. Covid-19 numbers by an open SIR model with testing

Mathematics in Applied Sciences and Engineering
The U.S. COVID-19 data exhibit a high-frequency oscillation along a low-frequency wave for outbreaks. A modified SIR model is proposed to explain this spiking phenomenon. It is also used to fit the data and to make a  forecast.
Bo Deng
doaj   +1 more source

Application of Intelligent Systems in CO2 Management: State of the Art and Future Prospects

CAAI Transactions on Intelligence Technology, EarlyView.
ABSTRACT Machine learning (ML) integration is becoming increasingly popular in advancing the 4th industrial revolution, known as Industry 4.0. This review paper examines ML applications in CO2 management stages: emission, capture, and conversion. ML models, including multiple linear regression (MLR), multiple nonlinear regression (MNLR), and artificial
Muhammad Zulkefal, Iftikhar Ahmad, Asad Habib, Asad Ayub, Hamza Sethi, Ihsanullah Ihsanullah, Hakan Caliskan, Hiki Hong   +7 more
wiley   +1 more source

Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern

Antibiotics, 2020
Forty-eight Pasteurella multocida isolates were recovered from porcine pneumonic lungs collected from farms in “Castilla y León” (north-western Spain) in 2017–2019.
Máximo Petrocchi-Rilo, César-B. Gutiérrez-Martín, Esther Pérez-Fernández, Anna Vilaró, Lorenzo Fraile, Sonia Martínez-Martínez   +5 more
doaj   +1 more source

Band‐Pass Filtering With High‐Dimensional Time Series. A Synthetic Indicator of the Medium‐to‐Long Run Component of Growth

Journal of Time Series Analysis, EarlyView.
ABSTRACT The paper deals with the construction of a synthetic indicator of economic growth, obtained by projecting a quarterly measure of aggregate economic activity, namely gross domestic product (GDP), into the space spanned by a finite number of smooth principal components, representative of the medium‐to‐long‐run component of economic growth of a ...
Alessandro Giovannelli, Marco Lippi, Tommaso Proietti   +2 more
wiley   +1 more source

Seasonal Restructuring of Microbial Communities and Resistomes in the Shitalakshya River, Bangladesh Revealed by Shotgun Metagenomics

MicrobiologyOpen, Volume 15, Issue 4, August 2026.
Dry‐season pollutant concentration drives microbial restructuring in Bangladesh's Shitalakshya River, shifting communities from Myroides to stress‐tolerant genera while enriching metal resistance genes and efflux pumps, revealing non‐antibiotic co‐selection as a key driver of antimicrobial resistance in urban waters.
Muhammad Ehteshamul Haque, Md. Shaminur Rahman, Munawar Sultana, Anowara Begum   +3 more
wiley   +1 more source

Improvements of the Reactor Dynamics Code RESTA3D and Its Application to the 2 MWth TMSR Transient Safety Analysis

Energies
Molten salts act as fuel carriers and coolants in liquid-fueled molten salt reactors (MSRs), characterized by strong coupling between neutronics and thermal hydraulics (N-TH) in practical MSR operations.
Kailong Wang, Chunyan Zou, Ao Zhang, Yafen Liu, Yong Cui, Jingen Chen, Xiangzhou Cai   +6 more
doaj   +1 more source

Application of fluorescence spectrum to precisely inverse paddy rice nitrogen content

Plant, Soil and Environment, 2015
Paddy rice is important for Chinese agriculture and crop production, which largely depends on the leaf nitrogen (N) levels. The purpose of this study is to discuss the relationship between the fluorescence parameters and leaf N content of paddy rice and ...
J. Yang, S. Shi, W. Gong, L. Du, Y.Y. Ma, B. Zhu, S.L. Song   +6 more
doaj   +1 more source