Results 51 to 60 of about 2,250,294 (367)

Pitfalls and other issues with the MTT assay

Photodiagnosis and Photodynamic Therapy
While the colorimetric assay commonly referred to as ‘MTT’ does provide an indication of effects of photodamage on mitochondrial enzyme levels, a careful analysis showed that the resulting data were not correlated with a clonogenic assay.
David Kessel
doaj   +1 more source

Changes in 2-fluoro-2-deoxy-D-glucose incorporation, hexokinase activity and lactate production by breast cancer cells responding to treatment with the anti-HER-2 antibody trastuzumab [PDF]

, 2010
Peer reviewedPublisher ...
Cheyne, Richard, W., McLaughlin, Abbie, Smith, Timothy Andrew Davies, Trembleau, Laurent Alain Claude   +3 more
core   +1 more source

Cannabidiol stimulates AML-1a-dependent glial differentiation and inhibits glioma stem-like cells proliferation by inducing autophagy in a TRPV2-dependent manner [PDF]

, 2015
Glioma stem-like cells (GSCs) correspond to a tumor cell subpopulation, involved in glioblastoma multiforme (GBM) tumor ini- tiation and acquired chemoresistance.
Amantini, Consuelo, Liberati, Sonia, Morelli, MARIA BEATRICE, Nabissi, Massimo, Pallini, Roberto, Ricci Vitiani, Lucia, Santoni, Giorgio, Santoni, Matteo   +7 more
core   +2 more sources

Luteolin and Vernodalol as Bioactive Compounds of Leaf and Root Vernonia amygdalina Extracts: Effects on α-Glucosidase, Glycation, ROS, Cell Viability, and In Silico ADMET Parameters

Pharmaceutics, 2023
The aqueous decoctions of Vernonia amygdalina (VA) leaves and roots are widely used in traditional African medicine as an antidiabetic remedy. The amount of luteolin and vernodalol in leaf and root extracts was detected, and their role was studied ...
Francine Medjiofack Djeujo, Valentina Stablum, Elisa Pangrazzi, Eugenio Ragazzi, Guglielmina Froldi   +4 more
doaj   +1 more source

Elevated intracellular cAMP exacerbates vulnerability to oxidative stress in optic nerve head astrocytes. [PDF]

, 2018
Glaucoma is characterized by a progressive loss of retinal ganglion cells and their axons, but the underlying biological basis for the accompanying neurodegeneration is not known.
Bu, Jung Hyun, Ellisman, Mark H, Jeong, Seung Won, Ju, Won-Kyu, Kim, Keun-Young, Nam, Hye Seung, Park, Tae Lim, Shim, Myoung Sup, Weinreb, Robert N   +8 more
core   +2 more sources

The Effect of Bioceramic-Based Root Canal Sealers on the Cell Viability and Cytotoxicity of Human Periodontal Ligament Fibroblast cell line (An in vitro study) [PDF]

Al-Rafidain Dental Journal
The current study aimed to assess the cell viability and cytotoxicity of bioceramic-based root canal sealers by comparing them to a zinc oxide-eugenol-based sealer (Endovit) in the set state of human periodontal ligament fibroblasts for one day, three ...
Marwa Ahmed Jassim, Alaa E. Dawood, James Holden, Eric Reynolds   +3 more
doaj   +1 more source

Powerful Antioxidants and Cytotoxic Activities of the Methanol Extracts from Eight Soybean Cultivars

Molecules, 2022
In the present study, the chemical composition and total phenolic (TPC) and total flavonoid contents (TFC) of eight soybean cultivars (Giza 21, Giza 22, Giza 35, Giza 111, Giza 82, Giza 83, Crawford, and Holliday) were estimated.
Mohamed A. Abd Elhamid, Abd Elrahman S. Mandour, Tarek A. Ismail, Ahmed M. Al-Zohairy, Sanaa Almowallad, Leena S. Alqahtani, Ali Osman   +6 more
doaj   +1 more source

PARP inhibitors elicit distinct transcriptional programs in homologous recombination competent castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
PARP inhibitors are used to treat a small subset of prostate cancer patients. These studies reveal that PARP1 activity and expression are different between European American and African American prostate cancer tissue samples. Additionally, different PARP inhibitors cause unique and overlapping transcriptional changes, notably, p53 pathway upregulation.
Moriah L. Cunningham, Jasibel Vasquez‐Gonzalez, Samantha M. Barnada, Salome Tchotorlishvili, Latese Jones, Ryan Maguire, Genevieve Lewis, Kinza Rizwan, Jenny Deng, Salma Koachar, Drithi Patel, Hailey Shankle, Tessa Mulders, Namra Ajmal, Charalambos Solomides, Emad S. Alnemri, Teresa F. Alnemri, Ayesha A. Shafi, Leonard G. Gomella, Wm Kevin Kelly, Steven B. McMahon, Matthew J. Schiewer   +21 more
wiley   +1 more source

MTT assay v1

, 2022
The MTT assay is based on the conversion of MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide into purple formazan crystals by LIVINGcells, which determines the mitochondrial activity of these cells. Viable cells contain NADPH-dependent oxidoreductase enzymes which reduce MTT to formazan.1The insoluble formazan crystals ...
openaire   +1 more source

Adenosine‐to‐inosine editing of miR‐200b‐3p is associated with the progression of high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
A‐to‐I editing of miRNAs, particularly miR‐200b‐3p, contributes to HGSOC progression by enhancing cancer cell proliferation, migration and 3D growth. The edited form is linked to poorer patient survival and the identification of novel molecular targets.
Magdalena Niemira, Anna Skwarska, Karolina Chwialkowska, Agnieszka Ostrowska, Gabriela Sokolowska, Anna Zeller, Anna Erol, Andrzej Eljaszewicz, Bartosz Hanczaruk, Anna Michalska‐Falkowska, Agnieszka Tarasik, Joanna Reszec‐Gielazyn, Pawel Knapp, Marcin Moniuszko, Adam Kretowski   +14 more
wiley   +1 more source