Results 41 to 50 of about 227,429 (291)

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

open access: yesMolecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang   +12 more
wiley   +1 more source

MTT Assay in HeLa cells. [PDF]

open access: yes, 2016
The MTT assay was carried out after plating HeLa cells at a density of 5000 cells/well in 96 well plates. The cells were treated with different doses of deguelin. It shows a regression curve of cell viability at the indicated doses.
Ashraf Ali (625827)   +15 more
core   +1 more source

Effect of growth media on the MTT colorimetric assay in bacteria.

open access: yesPLoS ONE, 2019
Reduction of tetrazolium salts to colored formazan products by metabolically active cells is widely used for assessment of cell viability. Among the tetrazolium compounds most commonly used is MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium ...
Ludmil Benov
doaj   +1 more source

MTT (Assay protocol v1

open access: yes, 2023
MTT ((3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)) is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble MTT to an insoluble purple formazan. AMJ13: 2 (Ahmed.Murtuda ,Jabria 2013)cell line: The cell line of breast cancer has been obtained from Iraqi breast cancer which ...
Abdulkareem Hameed Abd   +2 more
openaire   +1 more source

Adaptor protein CIN85 potentiates the motility of osteosarcoma cells via the Akt/mTOR and MMP2‐COL3A1 axis

open access: yesMolecular Oncology, EarlyView.
CIN85 is highly expressed in osteosarcoma, particularly in metastatic lesions. Its overexpression increases cell migration and Matrigel invasion, while silencing CIN85 suppresses these behaviors. Transcriptome analysis shows that CIN85 regulates MMP2, COL3A1, and Akt/mTOR signaling. Targeting these pathways reverses CIN85‐induced motility, highlighting
Iryna Horak   +10 more
wiley   +1 more source

Cytotoxicity MTT assay. Virtual Lab video [PDF]

open access: yes, 2021
Cytotoxicity MTT assay. Virtual Lab video.FCT/MCTES através de fundos nacionais (PIDDAC), PTDC/SAU-PUB/29481/2017, PTDC/SAU-PUB/32587/2017 e ToxOmics ( Centre for Toxicogenomics and Human Health, UIDB/00009/2020)N/
Rolo, Dora   +7 more
core  

Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy

open access: yesMolecular Oncology, EarlyView.
Here, we demonstrate that HS1BP3 interacts with Cortactin through a proline‐rich region (PRR3.1) and show that this interaction, and HS1BP3 itself, promote cancer cell proliferation and invasion. Inhibition of this interaction leads to build‐up of TKS5 in multivesicular endosomes and altered secretion of CD63 and CD9, providing an explanation for the ...
Arja Arnesen Løchen   +9 more
wiley   +1 more source

IC50 from MTT assay (μM). [PDF]

open access: yes, 2015
NCI-ADR/res an A2780 were treated with MT-4, paclitaxel, and vincristine at different concentrations for cell viability. The IC50 was evaluated and compared to control by MTT assay after 48 h.IC50 from MTT assay (μM).
Shiow Lin Pan (723354)   +5 more
core   +1 more source

Chemical compositions and antifungal activities of Satureja macrosiphon against Candida and Aspergillus species [PDF]

open access: yesCurrent Medical Mycology, 2019
Background and Purpose: Despite the various applications of Satureja species, there are limited data in this domain. Regarding this, the present study was conducted to investigate the essential oil (EO) biological activity of S.
Marjan Motamedi Motamedi   +7 more
doaj   +1 more source

MTT assay v1

open access: yes, 2022
The MTT assay is based on the conversion of MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide into purple formazan crystals by LIVINGcells, which determines the mitochondrial activity of these cells. Viable cells contain NADPH-dependent oxidoreductase enzymes which reduce MTT to formazan.1The insoluble formazan crystals ...
openaire   +1 more source

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