Results 61 to 70 of about 167,879 (293)
The isolation, purification, tissue localization and identification of a glycoprotein found in the crude mucus gel of patients with carcinoma of the stomach [PDF]
, 2008 Includes bibliographical references (leaves 101-138).The thin layer of crude mucus lining the human gastric mucosa protects the delicate gastric epithelium from the high shear forces associated with digestion. Gastric mucus is composed largely of water (>
Nthato, Chirwa
core +1 more source
Molecular Oncology, EarlyView.This paper shows that size‐exclusion chromatography and sequential centrifugation applied to seminal plasma enrich for different populations of extracellular vesicles. This may significantly affect the detection and analysis of extracellular vesicle‐associated cancer biomarkers.
Elen Telumyan +8 more
wiley +1 more source
Frontiers in Microbiology, 2018 Dietary and host glycans shape the composition of the human gut microbiota with keystone carbohydrate-degrading species playing a critical role in maintaining the structure and function of gut microbial communities.
Emmanuelle H. Crost +5 more
doaj +1 more source
Marine Drugs, 2020 The mucus layer of the nasopharynx and bronchial epithelium has a barrier function against inhaled pathogens such as the coronavirus SARS-CoV-2. We recently found that inorganic polyphosphate (polyP), a physiological, metabolic energy (ATP)-providing ...
Werner E. G. Müller +5 more
doaj +1 more source
Tumour‐proximal deep serum proteomics identifies biomarkers associated with ovarian cancer
Molecular Oncology, EarlyView.New biomarkers are needed for early ovarian cancer detection. Ovarian (tumour‐proximal) and peripheral vein serum from healthy women and patients with benign or malignant tumours underwent nanoparticle‐enriched deep proteomics. Ovarian serum yielded more protein identifications and revealed tumour‐reflective signatures.
Darren Lau +9 more
wiley +1 more source
Expression and purification of mucin 16 and preparation and characterization of anti-mucin 16 monoclonal antibody [PDF]
, 2012 目的:在原核生物中表达带有HIS标签的MuCIn 16n端重组蛋白(简称为HIS-MuCIn 16n),制备抗MuCIn 16的单克隆抗体(MAb)。方法:将MuCIn 16基因片段插入原核表达载体PET-32,在大肠杆菌中表达重组蛋白,用亲和纯化方法纯化后免疫bAlb/C小鼠,并进行细胞融合。筛选可稳定分泌抗MuCIn 16抗体的阳性单克隆杂交瘤细胞株,用WESTErnblOT、ElISA、免疫荧光和免疫组化等方法分析和鉴定抗MuCIn16的MAb。结果:表达并纯化了HIS-MuCIn 16n蛋白 ...
丁宇婷 +4 more
core +2 more sources
Cells, 2019 Pancreatic cancer (PanCa) is one of the leading causes of death from cancer in the United States. The current standard treatment for pancreatic cancer is gemcitabine, but its success is poor due to the emergence of drug resistance.
Mohammed Sikander +10 more
doaj +1 more source
Cell surface CD11c as a neutrophil aging marker molecule
FEBS Open Bio, EarlyView.Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki +5 more
wiley +1 more source
Novel Sustained-Release Drug Delivery System for Dry Eye Therapy by Rebamipide Nanoparticles
Pharmaceutics, 2020 The commercially available rebamipide ophthalmic suspension (CA-REB) was approved for clinical use in patients with dry eye; however, the residence time on the ocular surface for the traditional formulations is short, since the drug is removed from the ...
Noriaki Nagai +8 more
doaj +1 more source
Gut microbiota shape the inflammatory response in mice with an epithelial defect
Gut Microbes, 2021 Intestinal epithelial cell endoplasmic reticulum (ER) stress has been implicated in intestinal inflammation. It remains unclear whether ER stress is an initiator of or a response to inflammation.
Ran Wang +10 more
doaj +1 more source