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Inaccurate Multilocus Sequence Typing of Acinetobacter baumannii [PDF]

open access: yesEmerging Infectious Diseases, 2019
Multilocus sequence typing has been useful for genotyping pathogens in surveillance and epidemiologic studies. However, it cannot reflect the true relationships of isolates for species with very dynamic genomes.
Santiago Castillo-Ramírez   +1 more
doaj   +4 more sources

Multilocus Sequence Typing of Total-Genome-Sequenced Bacteria [PDF]

open access: yesJournal of Clinical Microbiology, 2012
ABSTRACT Accurate strain identification is essential for anyone working with bacteria. For many species, multilocus sequence typing (MLST) is considered the “gold standard” of typing, but it is traditionally performed in an expensive and time-consuming manner.
M. Larsen   +10 more
semanticscholar   +4 more sources

Multilocus Sequence Typing Tool for Cyclospora cayetanensis [PDF]

open access: yesEmerging Infectious Diseases, 2016
Because the lack of typing tools for Cyclospora cayetanensis has hampered outbreak investigations, we sequenced its genome and developed a genotyping tool. We observed 2 to 10 geographically segregated sequence types at each of 5 selected loci.
Yaqiong Guo   +12 more
doaj   +3 more sources

A multilocus sequence typing scheme for Mycobacterium abscessus complex (MAB-multilocus sequence typing) using whole-genome sequencing data

open access: yesInternational Journal of Mycobacteriology, 2019
Background:Mycobacterium abscessus is a rapid growing nontuberculous mycobacteria (NTM) and a clinically significant pathogen capable of causing variable infections in humans that are difficult to treat and may require months of therapy/surgical ...
Michelle Wuzinski   +5 more
doaj   +3 more sources

Consensus Multilocus Sequence Typing Scheme for Pneumocystis jirovecii [PDF]

open access: yesJournal of Fungi, 2020
Pneumocystis jirovecii is an opportunistic human pathogenic fungus causing severe pneumonia mainly in immunocompromised hosts. Multilocus sequence typing (MLST) remains the gold standard for genotyping of this unculturable fungus.
Lana Pasic   +8 more
doaj   +5 more sources

Multilocus Sequence Typing of Mycoplasma pneumoniae, Japan, 2002–2016

open access: yesEmerging Infectious Diseases, 2018
In Japan, Mycoplasma pneumoniae resistance to macrolides is high. To compare sequence types (STs) of susceptible and resistant isolates, we performed multilocus sequence typing for 417 isolates obtained in Japan during 2002–2016.
Mariko Ando   +4 more
doaj   +2 more sources

Multilocus Sequence Typing of Klebsiella pneumoniae Nosocomial Isolates [PDF]

open access: yesJournal of Clinical Microbiology, 2005
ABSTRACT A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae . Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates.
L. Diancourt   +4 more
semanticscholar   +4 more sources

Multilocus sequence typing breathes life into a microbial metagenome. [PDF]

open access: yesPLoS ONE, 2006
Shot-gun sequencing of DNA isolated from the environment and the assembly of metagenomes from the resulting data has considerably advanced the study of microbial diversity.
Eshwar Mahenthiralingam   +6 more
doaj   +5 more sources

Optimized multilocus sequence typing (MLST) scheme for Trypanosoma cruzi. [PDF]

open access: yesPLoS Neglected Tropical Diseases, 2014
Trypanosoma cruzi, the aetiological agent of Chagas disease possess extensive genetic diversity. This has led to the development of a plethora of molecular typing methods for the identification of both the known major genetic lineages and for more fine ...
Patricio Diosque   +13 more
doaj   +8 more sources

refMLST: reference-based multilocus sequence typing enables universal bacterial typing

open access: yesBMC Bioinformatics
Background Commonly used approaches for genomic investigation of bacterial outbreaks, including SNP and gene-by-gene approaches, are limited by the requirement for background genomes and curated allele schemes, respectively.
Mondher Khdhiri   +7 more
doaj   +3 more sources

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