Results 21 to 30 of about 21,339 (261)
Multiphoton microscopy imaging of developing tooth germs
Journal of the Formosan Medical Association, 2014 Traditionally, tooth germ is observed by histological investigation with hematoxylin and eosin stain and information may loss during the process. The purpose of this study is to use multiphoton laser fluorescence microscopy to observe the developing ...
Pei-Yu Pan +5 more
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Machine Learning with Applications, 2021 In this work, the use of transfer learning was explored to achieve the overall goal of classifying a small number (approx. 215 images) of Nonlinear Multiphoton Multimodal Microscopy Images of unstained oral cancer biopsies into three categories—healthy ...
Gabrielle A. Murashova, Dirk Colbry
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Recent Advances in Intravital Imaging of Dynamic Biological Systems
Journal of Pharmacological Sciences, 2012 Intravital multiphoton microscopy has opened a new era in the field of biological imaging. Focal excitation of fluorophores by simultaneous attack of multiple (normally “two”) photons generates images with high spatial resolution, and use of near ...
Junichi Kikuta, Masaru Ishii
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Multiphoton intravital microscopy of rodents
Nature Reviews Methods Primers, 2022 Tissues are heterogeneous with respect to cellular and non-cellular components and in the dynamic interactions between these elements. To study the behaviour and fate of individual cells in these complex tissues, intravital microscopy (IVM) techniques such as multiphoton microscopy have been developed to visualize intact and live tissues at cellular ...
Colinda L. G. J. Scheele +12 more
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Multiphoton FLIM and spectral imaging of cells and tissues
, 2022 S.240-251Five-dimensional (5D) multiphoton measurements with submicron spatial resolution, 270 ps temporal resolution and 5 nm spectral resolution have been performed on living cells and tissues at 750 nm - 850 nm laser excitation. A compact (65×62×48 cm(
Ehrlich, G. +4 more
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Simultaneous imaging of GFP, CFP and collagen in tumors
BMC Biotechnology, 2005 Background The development of multiphoton laser scanning microscopy has greatly facilitated the imaging of living tissues. However, the use of genetically encoded fluorescent proteins to distinguish different cell types in living animals has not been ...
Segall Jeffrey E +5 more
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Intravital imaging of orthotopic and ectopic bone
Inflammation and Regeneration, 2020 Bone homeostasis is dynamically regulated by a balance between bone resorption by osteoclasts and bone formation by osteoblasts. Visualizing and evaluating the dynamics of bone cells in vivo remain difficult using conventional technologies, including ...
Kunihiko Hashimoto +3 more
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Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae
Scientific Reports, 2021 Imaging in three dimensions is necessary for thick tissues and small organisms. This is possible with tomographic optical microscopy techniques such as confocal, multiphoton and light sheet microscopy.
Dimitrios Kapsokalyvas +7 more
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Real-Time Multidepth Multiphoton Microscopy Using Pixel-to-Pixel Focus-Switching
Applied Sciences, 2020 Multiphoton microscopy is a well-established technique for biomedical applications, but real-time multidepth multimodal multiphoton microscopy using non-imaging detection has barely been discussed. We demonstrate a novel label-free imaging system capable
Yifan Qin, Deying Chen, Yuanqin Xia
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PLoS ONE, 2012 The progressive accumulation of monocyte-derived cells in the atherosclerotic plaque is a hallmark of atherosclerosis. However, it is now appreciated that monocytes represent a heterogeneous circulating population of cells that differ in functionality ...
Abigail S Haka +4 more
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