Results 51 to 60 of about 21,339 (261)
Applications of combined spectral lifetime microscopy for biology
BioTechniques, 2006 Live cell imaging has been greatly advanced by the recent development of new fluorescence microscopy-based methods such as multiphoton laser-scanning microscopy, which can noninvasively image deep into live specimens and generate images of extrinsic and ...
Long Yan +3 more
doaj +1 more source
Ultracompact autocorrelator for multiphoton microscopy
Microscopy Research and Technique, 2003 AbstractPulse temporal characterization is a fundamental task when operating a Ti:Sapphire ultrafast laser system for multiphoton microscopy applications. In the present report, an ultracompact autocorrelator setup and a simple procedure is reported to perform pulse width measurements at the focal plane of the microscope objective without the need of ...
Quercioli F +3 more
openaire +2 more sources
FEBS Open Bio, EarlyView.Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas +6 more
wiley +1 more source
Multiphoton plasmon-resonance microscopy
Optics Express, 2003 A novel method for detection of noble-metal nanoparticles by their nonlinear optical properties is presented and applied for specific labeling of cellular organelles. When illuminated by laser light in resonance with their plasmon frequency these nanoparticles generate an enhanced multiphoton signal.
Dvir, Yelin +4 more
openaire +2 more sources
Advanced Healthcare Materials, EarlyView.Coagulative granular hydrogels are composed of packed thrombin‐functionalized microgels that catalyze the conversion of fibrinogen into a secondary fibrin network, filling the interstitial voids. This bio‐inspired approach stabilizes the biomaterial to match the robustness of bulk hydrogels without compromising injectability, mimicking the initial ...
Zhipeng Deng +16 more
wiley +1 more source
IEEE Photonics Journal, 2018 Near infrared (NIR) excitation or emission is capable of deep-tissue penetration in various modalities of optical microscopy. Based on the transmittance characterization of biological tissue, such as brains, four NIR optical windows have been ...
Ke Wang +5 more
doaj +1 more source
Advanced Healthcare Materials, EarlyView.Microgel‐based 3D printed constructs represent a compelling and versatile innovation for engineering architecturally complex, dynamically remodelable, and biocompatible structures with high structural fidelity and bioactivity. By integrating material design, biofabrication, and biological function, these systems enable the development of adaptive ...
Elena Ghighină +2 more
wiley +1 more source
Multi messenger multiphoton microscopy (Conference Presentation)
, 2019 Multiphoton microscopy was more than a revolution in optical microscopy and more in general in optical bioimaging. In the last 25 years has enabled unprecedented exploration of biological systems including living organisms.
Diaspro, Alberto
core +1 more source
Neutrophil‐Mimetic MRI Enables Ultra‐Early Detection of Vascular Inflammation After Stroke
Advanced Healthcare Materials, EarlyView.In this study, we developed neutrophil‐mimetic MRI probes that detect ultra‐early neuroinflammation following ischemic stroke by targeting E‐selectin. Within seconds of their injection, these microparticles mimic initial leukocyte adhesion to the activated cerebral endothelium.
Marion Isabelle Morvan +17 more
wiley +1 more source
Methodology for reconstructing early zebrafish development from in vivo multiphoton microscopy
, 2012 Investigating cell dynamics during early zebrafish embryogenesis requires specific image acquisition and analysis strategies. Multiharmonic microscopy, i.e., second- and third-harmonic generations, allows imaging cell divisions and cell membranes in ...
A. Santos +30 more
core +1 more source