Results 81 to 90 of about 21,339 (261)

Intrinsic indicator of photodamage during label-free multiphoton microscopy of cells and tissues.

PLoS ONE, 2014
Multiphoton imaging has evolved as an indispensable tool in cell biology and holds prospects for clinical applications. When addressing endogenous signals such as coherent anti-Stokes Raman scattering (CARS) or second harmonic generation, it requires ...
Roberta Galli, Ortrud Uckermann, Elisabeth F Andresen, Kathrin D Geiger, Edmund Koch, Gabriele Schackert, Gerald Steiner, Matthias Kirsch   +7 more
doaj   +1 more source

Subcellular analysis of blood-brain barrier function by micro-impalement of vessels in acute brain slices

Nature Communications, 2023
The blood-brain barrier (BBB) tightly and dynamically regulates exchange with the brain. Here, the authors report a brain slice approach to study the native BBB in a highly controlled manner by combining intravascular perfusion and multiphoton microscopy.
Amira Sayed Hanafy, Pia Steinlein, Julika Pitsch, Mariella Hurtado Silva, Natascha Vana, Albert J. Becker, Mark Evan Graham, Susanne Schoch, Alf Lamprecht, Dirk Dietrich   +9 more
doaj   +1 more source

3D‐Nanoprinted Fluidically Steerable Soft Robotic Microcatheters

Advanced Robotics Research, EarlyView.
This work introduces a ∼3‐French (1 mm‐in‐diameter) soft robotic microcatheter fabricated by means of two‐photon direct laser writing. The 3D‐printed microcatheter enables remote, on‐demand steering via microfluidic actuation for guidewire‐free navigation of vascular networks as well as microcatheter‐mediated delivery of fluidic payloads to target ...
Bailey M. Felix, Molly Carton, Olivia M. Young, Noah Barnes, Jordi T. Andreou, Sarah McHugh, Jack E. McCann, Claudia Davis, Victoria Reddig‐Leiman, Afsah Kamran, Dheeraj Gandhi, Miroslaw Janowski, Jeremy D. Brown, Mark Fuge, Axel Krieger, Clifford R. Weiss, Christopher R. Bailey, Ryan D. Sochol   +17 more
wiley   +1 more source

Measurements of multiphoton action cross sections for multiphoton microscopy

Biomedical Optics Express, 2014
We report quantitative measurements of two-, three-, and four-photon excitation action cross sections of several commonly used fluorophores and fluorescent proteins at three different excitation wavelengths of 800 nm, 1300 nm, and 1680 nm. The measured cross section values are consistent with simple quantum mechanic estimations.
Li-Chung, Cheng, Nicholas G, Horton, Ke, Wang, Shean-Jen, Chen, Chris, Xu   +4 more
openaire   +2 more sources

Mitochondria and Neuromast Tagging With Fluorescent Gallium‐Triapine Analogues: In Cellulo MP FLIM and Zebrafish Live Imaging

Advanced Science, EarlyView.
Fluorescent BODIPY‐conjugated thiosemicarbazone ligands and their Ga(III), In(III), and Fe(III) complexes, inspired by Triapine, are developed as theranostic agents. Multiphoton FLIM and confocal microscopy in cancer cells and zebrafish reveal real‐time uptake, mitochondrial localisation, and whilst spectroscopic assays indicated preserved complex ...
Megan J. Green, Michael W. Jones, Merissa Saleem, Haobo Ge, Melita Tvardauskaite, Julia Dudzic, Fernando Cortezon‐Tamarit, Rory L. Arrowsmith, Charareh Pourzand, Gabriele Kociock‐Kohn, Nicholas H. Rees, Jonathan R. Dilworth, Stephen Faulkner, Stanley W. Botchway, David Gurevich, Sofia I. Pascu   +15 more
wiley   +1 more source

Multiphoton microscopy in brain imaging

Brain imaging is becoming an important field in the frame of the neurophotonics in correlations with other medical ones in neuroscience studying functional and morphological aspects.
Pavone, F. S., Maco, B., Silvestri, L., Sacconi, L., Mascaro, A. L. Allegra, Costantini, I., Knott, G. W.   +6 more
core   +1 more source

Advances in imaging of new targets for pharmacological intervention in stroke: real-time tracking of T-cells in the ischaemic brain [PDF]

, 2010
Background and purpose: T‐cells may play a role in the evolution of ischaemic damage and repair, but the ability to image these cells in the living brain after a stroke has been limited.
Brewer JM, De Simoni, M.G., Millington OR, Rodolico G, Bushell, T.J., Dever G, Dever, G., Brewer, J.M., Bushell, T., Ortolano, F., Maffia, P., Millington, O.R., Ortolano F, Rodolico, G., Carswell H.V., Garside P, Garside, P., Carswell, H.V., Maffia, Pasquale, De Simoni MG, Bushell TJ, Carswell, H.V.O.   +21 more
core   +1 more source

Multiparametric high-resolution imaging of barley embryos by multiphoton microscopy and magnetic resonance micro-imaging

, 2022
S.426-432Nonlinear optical microscopy and magnetic resonance imaging (MRI) address different properties of the sample and operate on different geometrical scales.
Ehlers, A., Volke, F., Manz, B., Weschke, W., Stark, M., Küppers, M., Siebert, U., Riemann, I., König, K.   +8 more
core   +1 more source

Extreme Polaritonic Interactions in a Room‐Temperature Designable Sub‐Nanocavity Quantum Electrodynamic Platform

Advanced Science, EarlyView.
 A nanoparticle‐cluster‐on‐mirror (NPcoM) serves as a functionally deterministic sub‐nanocavity that emulates picocavities, enabling extreme light–matter interactions and polaritonic phenomena. ABSTRACT Pushing nanoscale optical confinement to its ultimate limits defines the regime of nano‐cavity quantum electrodynamics (nano‐cQED), where light‐matter ...
Huatian Hu, Xin Shu, Zhiwei Hu, Di Zheng, Ruiqian Zhang, Ximin Cui, Wei Dai, Xiang Lan, Xiaobo Han, Wen Chen, Hongxing Xu   +10 more
wiley   +1 more source

Challenges and Prospects of Using Novel Nonlinear Effects in Multimode Optical Fibers for Multiphoton Endomicroscopy

Diagnostics
Multiphoton endomicroscopy (MPEM) has recently become a key development in optical biomedical diagnostics, providing histologically relevant in vivo images that are eliminating both the need for tissue damage during biopsy sampling and the need for dye ...
Lidiya V. Boldyreva, Denis S. Kharenko, Kirill V. Serebrennikov, Anna A. Evtushenko, Viktor V. Shloma, Daba A. Radnatarov, Alexandr V. Dostovalov, Zhibzema E. Munkueva, Oleg S. Sidelnikov, Igor S. Chekhovskoy, Kirill S. Raspopin, Mikhail D. Gervaziev, Stefan Wabnitz   +12 more
doaj   +1 more source