Microfluidics-based approaches to the isolation of African trypanosomes [PDF]
, 2017 African trypanosomes are responsible for significant levels of disease in both humans and animals. The protozoan parasites are free-living flagellates, usually transmitted by arthropod vectors, including the tsetse fly. In the mammalian host they live in
Barrett, Michael P. +6 more
core +3 more sources
Development and comparison of a novel multiple cross displacement amplification (MCDA) assay with other nucleic acid amplification methods for SARS-CoV-2 detection [PDF]
Scientific Reports, 2020 AbstractThe development of alternative isothermal amplification assays including multiple cross displacement amplification (MCDA) may address speed and portability limitations of real-time PCR (rt-PCR) methods for SARS-CoV-2 detection. We developed a novel SARS-CoV-2 MCDA assay and compared its speed and sensitivity to loop-mediated isothermal ...
Wai Luu LD +4 more
europepmc +6 more sources
Ultraaccurate genome sequencing and haplotyping of single human cells. [PDF]
, 2017 Accurate detection of variants and long-range haplotypes in genomes of single human cells remains very challenging. Common approaches require extensive in vitro amplification of genomes of individual cells using DNA polymerases and high-throughput short ...
Bafna, Vineet +6 more
core +1 more source
Frontiers in Bioengineering and Biotechnology, 2023 Mycoplasma pneumoniae (MP), which is responsible for a majority of community-acquired pneumonia (CAP) in children, has been largely underestimated. Here, we coupled multiple cross displacement amplification (MCDA) technique with CRISPR-Cas12a-based ...
Nan Jia +9 more
doaj +1 more source
Multiple Cross Displacement Amplification Combined with Gold Nanoparticle-Based Lateral Flow Biosensor for Detection of Vibrio parahaemolyticus. [PDF]
Front Microbiol, 2016 Vibrio parahaemolyticus (V. parahaemolyticus) is a marine seafood-borne pathogen causing severe illnesses in humans and aquatic animals. In the present study, multiple cross displacement amplification was combined with a lateral flow biosensor (MCDA-LFB) to detect the toxR gene of V. parahaemolyticus in DNA extracts from pure cultures and spiked oyster
Wang Y +6 more
europepmc +4 more sources
Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]
, 2018 All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane +2 more
core +2 more sources
Signatures of galactic magnetic lensing upon ultra high energy cosmic rays [PDF]
, 2000 We analyse several implications of lensing by the regular component of the galactic magnetic field upon the observed properties of ultra high energy cosmic rays.
Harari, Diego +2 more
core +2 more sources
STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods [PDF]
, 2017 The growing interest in liquid biopsies for cancer research and cell-based non-invasive prenatal testing (NIPT) invigorates the need for improved single cell analysis.
Cornelis, Senne +5 more
core +2 more sources
Wave propagation through a coherently amplifying random medium [PDF]
, 1997 We report a detailed and systematic numerical study of wave propagation through a coherently amplifying random one-dimensional medium. The coherent amplification is modeled by introducing a uniform imaginary part in the site energies of the disordered ...
A. M. Jayannavar +3 more
core +2 more sources
Frontiers in Bioengineering and Biotechnology, 2021 Hepatitis B virus (HBV) is one of the most dangerous and prevalent agents that causes acute and chronic liver diseases in humans. Genotyping plays an important role in determining clinical outcomes and response to antiviral treatment in HBV–infected ...
Xu Chen +8 more
doaj +1 more source