Multiplex ligation-dependent probe amplification – a short overview
Romanian Journal of Laboratory Medicine, 2020 Multiplex Ligation-dependent Probe Amplification is a technique proposed for the detection of deletions or duplications that may lead to copy number variations in genomic DNA, mainly due to its higher resolution, and shorter overall diagnosis time, when ...
Moldovan Valeriu, Moldovan Elena
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Advances of multiplex ligation-dependent probe amplification technology in molecular diagnostics
BioTechniques, 2022 Multiplex ligation-dependent probe amplification (MLPA) is a multiplex copy number analysis tool which is routinely used to detect large mutations in genetic diseases.
Xiaoni Fu +7 more
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MAPD: a probe design suite for multiplex ligation-dependent probe amplification assays [PDF]
BMC Research Notes, 2010 Background Multiplex ligation-dependent probe amplification (MLPA) was originally described as an efficient and reliable technique for gene dosage or DNA copy number variation (CNV) analysis.
Zhi Jizu
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Incidental finding at methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA): how to proceed? [PDF]
Frontiers in Genetics, 2023 Introduction: Since the advent of new generation sequencing, professionals are aware of the possibility of obtaining findings unrelated to the pathology under study.
Africa Manero-Azua +4 more
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Multiplex ligation-dependent probe amplification using a completely synthetic probe set
BioTechniques, 2004 The recent development of multiplex ligation-dependent probe amplification (MLPA) has provided an efficient and reliable assay for dosage screening of multiple loci in a single reaction. However, a drawback to this method is the time-consuming process of
Rowena F. Stern +5 more
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Identification of nine genomic regions of amplification in urothelial carcinoma, correlation with stage, and potential prognostic and therapeutic value. [PDF]
PLoS ONE, 2013 We performed a genome wide analysis of 164 urothelial carcinoma samples and 27 bladder cancer cell lines to identify copy number changes associated with disease characteristics, and examined the association of amplification events with stage and grade of
Yvonne Chekaluk +8 more
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Probe-specific mixed-model approach to detect copy number differences using multiplex ligation-dependent probe amplification (MLPA) [PDF]
BMC Bioinformatics, 2008 Background MLPA method is a potentially useful semi-quantitative method to detect copy number alterations in targeted regions. In this paper, we propose a method for the normalization procedure based on a non-linear mixed-model, as well as a new approach
Villatoro Sergi +6 more
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Multiplex ligation-dependent probe amplification versus karyotyping in prenatal diagnosis: the M.A.K.E. study [PDF]
BMC Pregnancy and Childbirth, 2008 Background In the past 30 years karyotyping was the gold standard for prenatal diagnosis of chromosomal aberrations in the fetus. Traditional karyotyping (TKT) has a high accuracy and reliability.
Bhola Shama L +19 more
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Concordance of copy number abnormality detection using SNP arrays and Multiplex Ligation-dependent Probe Amplification (MLPA) in acute lymphoblastic leukaemia. [PDF]
Sci Rep, 2020 In acute lymphoblastic leukaemia, MLPA has been used in research studies to identify clinically relevant copy number abnormality (CNA) profiles. However, in diagnostic settings other techniques are often employed.
Bashton M +7 more
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Maternally inherited Leigh syndrome detected by Multiplex ligation-dependent probe amplification [PDF]
Mitochondrial DNA. Part B. Resources, 2019 Leigh syndrome (LS) is a mitochondrial progressive encephalopathy characterized by bilateral symmetric necrotic lesions of the central nervous system. Maternally inherited Leigh Syndrome (MILS) represents ∼10–20% of LS.
Lía Mayorga +7 more
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