Results 91 to 100 of about 29,886 (297)

Flipping the Switch: MeCP2‐Mediated Lactylation Rewires Microglial Metabolism and Inflammation via the HK2/mTOR Axis in Poststroke Neuroinflammation

Advanced Science, EarlyView.
Stroke‐induced lactate accumulation promotes p300‐mediated lactylation of methyl‐CpG binding protein 2 (MeCP2) at lysine 210, which reprograms microglial metabolism toward glycolysis and activates the hexokinase 2 (HK2)/mTOR axis. This cascade promotes proinflammatory responses and impairs neurofunctional outcomes.
Zengyu Zhang, Shanshan Huang, Yong Wang, Zhiwen Jiang, Zhuohang Liu, Chenran Wang, Rong Ji, Yiwen Yuan, Xueyu Mao, Kaicheng Yang, Huicong Niu, Yanqin Gao, Jing Zhao   +12 more
wiley   +1 more source

Sequencing data and MLPA analysis data in support of the effectiveness and reliability of an asymmetric PCR-Based approach in preparing long MLPA probes

Data in Brief, 2015
ABI PRISM 3100 Genetic Analyzer, a multi-color fluorescence-based DNA analysis system with 16 capillaries operating in parallel, was ideal tool both for DNA sequencing and DNA fragment analysis [1,2].
Xingyuan Ling, Hai Long, Guang Pan, Zhinan Chen   +3 more
doaj   +1 more source

Loss of SOCS1 in Donor T Cells Exacerbates Intestinal GVHD by Driving a Chemokine‐Dependent Pro‐Inflammatory Immune Microenvironment

Advanced Science, EarlyView.
T cell‐specific Socs1 knockout leads to inflammatory differentiation of CD8+ T cells, prompting the STAT1/2 complex to drive the activation of Ccl5, Ccr5, and Cxcr3, and promoting the skewing of monocytes toward a pro‐inflammatory M1 macrophage lineage.
Zhigui Wu, Bixia Wang, Xinya Jiang, Fangqing Zhang, Qianqian Huang, Xinyi Wu, Shuang Fan, Qi Zhang, Jingrui Zhou, Jianing Tang, Xiao‐Dong Mo, Yu Wang, Ying‐Jun Chang, Huidong Guo, Xiao‐Jun Huang   +14 more
wiley   +1 more source

Neurofibromatosis type 1: Splicing mutation detected by MLPA and DNA sequencing in Argentina [PDF]

, 2015
La neurofibromatosis tipo 1 (NF1) es un desorden genético autosómico dominante, con una prevalenciade 1 en 2500-3000 nacidos vivos. La dificultad diagnóstica se debe al tamaño extenso delgen NF1 con pocos sitios hot-spot, la ausencia de una clara ...
Dipierri, Jose Edgardo, Laurito, Sergio Roberto, Roqué, María   +2 more
core  

Single‐Cell Profiling Across Immune Tissues and Organs Reveals Immunosenescence Signatures in Male Rhesus Monkeys

Advanced Science, EarlyView.
Single‐cell profiling across bone marrow, spleen, mesenteric lymph, and blood in rhesus monkeys reveals organ Immunosenescence. GZMB rises with age, particularly in cytotoxic and terminally exhausted CD8+ T cells, and BHLHE40 emerges as a key transcription factor enriched across multiple CD8+ subsets, regulating pro‐inflammatory and exhaustion‐related ...
Shengnan Wang, Zhengna Zhu, Hongju Yang, Yaping Yan, Luyao Ran, Naixue Yang, Yichao Wang, Li Wu, Bing Bai, Weizhi Ji, Wei Si   +10 more
wiley   +1 more source

GlycoChat Uncovers Glycan–Lectin Circuits in the Tumor Microenvironment of Pancreatic Cancer

Advanced Science, EarlyView.
Aberrant glycosylation drives cancer progression, yet its role in the tumor microenvironment remains unclear. We developed GlycoChat to map glycan–lectin circuits at single‐cell resolution. We discovered that cancer cells induce immunosuppressive macrophage differentiation and impair phagocytosis through interactions with CLEC10A and SIGLEC3 ...
Dinh Xuan Tuan Anh, Sunanda Keisham, Arun Burramsetty, Lalhaba Oinam, Koichiro Kumano, Akihiro Kuno, Osamu Shimomura, Tatsuya Oda, Hiroaki Tateno   +8 more
wiley   +1 more source

Frequent Coamplification of Receptor Tyrosine Kinase and Downstream Signaling Genes in Japanese Primary Gastric Cancer and Conversion in Matched Lymph Node Metastasis [PDF]

, 2018
Objective: To establish the gene copy number status of receptor tyrosine kinase (RTK) and downstream signaling (DSS) genes genes in primary gastric cancer (primGC) and matched lymph node metastases (LNmet).
Alonso Guervos, Bang, Bardelli, Bozzetti, Bray, Brenner, Bunyan, Cepero, Chen, Cho, Das, de Souza, Deng, Dulak, Fuchs, Galizia, Gronwald, Henjes, Homig-Holzel, Huw, Japanese Gastric Cancer, Jung, Kamangar, Kim, Kochi, Lackner, Lauren, Lee, Liu, Lordick, Nagatsuma, Ooi, Ouyang, Peng, Scaltriti, Schouten, Stuppia, Tapia, Vaarwater, Vakiani, Valtorta, van Grieken, Waddell, Wang, Wong, Yan, Yonesaka   +46 more
core   +1 more source

The FOXC2‐LAMA4 Axis Orchestrates Vasculogenic Mimicry and Immunosuppressive Niche Formation to Drive Metastatic Cascade in Renal Cell Carcinoma

Advanced Science, EarlyView.
Advanced clear cell renal cell carcinoma metastasis is driven by a FOXC2+ tumor subpopulation. These cells execute vasculogenic mimicry for dissemination and secrete LAMA4. LAMA4 engages macrophage ITGA6, activating the STAT6/GATA3 axis to polarize macrophages into a pro‐metastatic, immunosuppressive phenotype.
Jiaxi Yao, Tong Xu, Chengyuan Wang, Chenyuan Wang, Junfeng Xie, Rui Zheng, Ke Wang, Xingzuo Jiang, Zewei Hu, Hongwei Jing, Lin Li, Tao Liu   +11 more
wiley   +1 more source

Novel visual detection technique of isothermal Ligation-dependent probe combined with hybridization chain reaction and hydrogel for the wild edible mushroom component

Applied Food Research
Multiplex ligation-dependent probe amplification (MLPA) is a high-throughput detection technology with superior specificity and sensitivity, which combines ligation-dependent probe hybridization and PCR amplification.
Nali Zhou, Hanyue Zhang, Shuna Xiang, Yingting Lin, Ying Shang   +4 more
doaj   +1 more source

Molecular Differences between Ductal Carcinoma In Situ and Adjacent Invasive Breast Carcinoma: A Multiplex Ligation-Dependent Probe Amplification Study

Analytical Cellular Pathology, 2010
Ductal carcinoma in situ (DCIS) accounts for approximately 20% of mammographically detected breast cancers. Although DCIS is generally highly curable, some women with DCIS will develop life-threatening invasive breast cancer, but the determinants of ...
Cathy B. Moelans, Roel A. de Weger, Hanneke N. Monsuur, Anoek H. J. Maes, Paul J. van Diest   +4 more
doaj   +1 more source