Results 131 to 140 of about 29,886 (297)

Multiplex ligation-dependent probe amplification and fluorescence in situ hybridization for detecting chromosome abnormalities in myelodysplastic syndromes: A retrospective study. [PDF]

Medicine (Baltimore), 2021
Ai X, Li B, Xu Z, Liu J, Qin T, Li Q, Xiao Z.   +6 more
europepmc   +1 more source

Multiplex ligation-dependent probe amplification MLPA) on-chip

, 2013
Polymerase chain reaction (PCR) has become an indispensable technique in clinical diagnostics. For integrated, lab-on-a-chip (LoC) solutions, multiplexed implementations of PCR are especially attractive; we describe the transfer and optimization of multiplex ligation-dependent probe amplification (MLPA) directly onto a silicon-based microfluidic chip ...
Peeters, Sara, Jones, Ben, Ibrahim, Omar, Wiederkehr, Rodrigo Sergio, Zhang, Lei, Tanaka, Hiroyuki, Matsuno, T., Yamashita, Ichiro, Majeed, Bivragh, Stakenborg, Tim, Fiorini, Paolo, Lagae, Liesbet   +11 more
openaire   +1 more source

A Subset of Pro‐inflammatory CXCL10+ LILRB2+ Macrophages Derives From Recipient Monocytes and Drives Renal Allograft Rejection

Advanced Science, EarlyView.
This study uncovers a recipient‐derived monocyte‐to‐macrophage trajectory that drives inflammation during kidney transplant rejection. Using over 150 000 single‐cell profiles and more than 850 biopsies, the authors identify CXCL10+ macrophages as key predictors of graft loss.
Alexis Varin, Jovanne Palvair, Lennie Messager, Jamal Bamoulid, Jasper Callemeyn, Mélanie Chaintreuil, Ludivine Dal Zuffo, Didier Ducloux, Imane Farhat, Mathieu Legendre, Laurent Martin, Florian Renosi, Xavier Roussel, Thibaut Vaulet, Maarten Naesens, Claire Tinel, Baptiste Lamarthée   +16 more
wiley   +1 more source

Genetic Diagnosis of Rubinstein-Taybi Syndrome With Multiplex Ligation-Dependent Probe Amplification (MLPA) and Whole-Exome Sequencing (WES): Case Series With a Novel CREBBP Variant. [PDF]

Front Genet, 2022
Lee YR, Lin YC, Chang YH, Huang HY, Hong YK, Aala WJF, Tu WT, Tsai MC, Chou YY, Hsu CK.   +9 more
europepmc   +1 more source

A TtAgo‐Driven Autocatalytic Circuit with Thermal‐Enhanced Kinetics for One‐Pot Nucleic Acid Detection

Advanced Science, EarlyView.
This study proposes a universal catalytic DNA circuits termed TACTIC (Thermus thermophilus protein‐driven autocatalytic circuit) for one‐pot detection of DNA and RNA in multiple clinical samples. Integrated with machine learning, TACTIC accurately profile the distinct expression of four extracellular vesicle‐derived miRNAs across different samples and ...
Zuowei Xie, Ruijia Deng, Ben Niu, Yingjie Yang, Shuang Zhao, Hongzhao Yang, Meilin Gong, Jie Luo, Yu Tang, Jing Sheng, Yan Pi, Ming Chen, Kai Chang   +12 more
wiley   +1 more source

Detection of Aneuploidies in Products of Conception and Neonatal Deaths in Iranian Patients Using the Multiplex Ligation-Dependent Probe Amplification (MLPA). [PDF]

Avicenna J Med Biotechnol, 2021
Khorami Sarvestani S, Rafati M, Soltanghoraee H, Hoseini A, Soltani A, Jalilian K, Ghaffari SR.   +6 more
europepmc   +1 more source

Compensatory Interplay Between Clarin‐1 and Clarin‐2 Deafness‐Associated Proteins Governs Phenotypic Variability in Hearing

Advanced Science, EarlyView.
Functional compensation between clarin‐1 and clarin‐2 in cochlear hair cells. Hearing loss associated with CLRN1 mutations shows striking phenotypic variability; however, the underlying mechanisms remain poorly understood. This study reveals that clarin‐1 and clarin‐2 function cooperatively in cochlear hair cells to sustain mechanoelectrical ...
Maureen Wentling, Aïda Yakhlef Sanchez, Nicolas Thelen, Müge Senarisoy, Maria Hogg, Steven Condamine, Andrea Lelli, Emilia Wysocka, Pranav Patni, Sandrine Vitry, Kerem Yasin Yildizhan, Sébastien Le Gal, Sylvie Nouaille, Michael R. Bowl, Marc Thiry, Didier Dulon, Sedigheh Delmaghani, Aziz El‐Amraoui   +17 more
wiley   +1 more source

Molecular Characterization of Ti Ringspot Associated Emara Virus and the Development of Assays for Its Detection [PDF]

, 2016
M.S. University of Hawaii at Manoa 2016.Includes bibliographical references.Ti ringspot disease (TRD) is an emerging virus-like disease of ti plants (Cordyline fruticosa L.) spreading throughout the Hawaiian Islands.
Park, Adam
core  

PIK3CA Mutations Downregulate PPT1 to Promote Adipogenesis by Suppressing P300 Depalmitoylation and Phase Separation

Advanced Science, EarlyView.
This study demonstrates that somatic PIK3CA mutations suppress PPT1 expression via activation of the PI3K–AKT–c‐JUN axis. This reduction in PPT1 weakens its interaction with P300, thereby increasing palmitoylation at C1176 of P300 and protecting P300 from lysosomal degradation.
Hongrui Chen, Zening Huang, Rui Chang, Wei Gao, Yajing Qiu, Bin Sun, Chen Hua, Xiaoxi Lin   +7 more
wiley   +1 more source

Studies on the prenatal diagnosis of spinal muscular atrophy by multiplex ligation⁃dependent probe amplification

Chinese Journal of Contemporary Neurology and Neurosurgery, 2012
Objective To investigate the value of multiplex ligation⁃dependent probe amplification (MLPA) method in the prenatal diagnosis of spinal muscular atrophy (SMA). Methods Six SMA pedigrees, which included 7 patients, 12 parents and 6 fetuses, were admitted
Ya⁃fang CHEN, Jin HE, Qi⁃jie ZHANG, Xiang LIN, Ning WANG, Wan⁃jin CHEN   +5 more
doaj