Results 251 to 260 of about 29,886 (297)

Multiplex ligation‐dependent probe amplification (MLPA) and prenatal diagnosis

Prenatal Diagnosis, 2012
ABSTRACTMultiplex ligation‐dependent probe amplification (MLPA) is a recent technique for the relative quantitation of up to 40 to 45 nucleic acid targets. Due to its relative simplicity, low cost, and availability of laboratory‐developed and more than 300 commercially‐developed assays, MLPA has become more widely used for both research and diagnostic ...
Alecia S, Willis, Ignatia, van den Veyver, Christine M, Eng   +2 more
openaire   +2 more sources

Methylation-specific multiplex ligation-dependent probe amplification in meningiomas

Journal of Neuro-Oncology, 2008
Genomic loss and promotor methylation contribute to inactivation of tumor suppressor genes (TSGs). Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) is a relatively new method for simultaneous detection of both these alterations. Here, we apply MS-MLPA to a series of 15 meningiomas of different WHO grades.
Christian, Ewald, Thomas, Hofmann, Susanne A, Kuhn, Thomas, Deufel, Christian, Beetz, Rolf, Kalff   +5 more
openaire   +2 more sources

Molecular diagnosis utility of multiplex ligation-dependent probe amplification

Expert Opinion on Medical Diagnostics, 2008
Multiplex ligation-dependent probe amplification (MLPA) is a novel method that uses multiple pairs of oligonucleotide probes to hybridize targeted genomic DNA regions; products from ligation reaction and polymerase chain reaction amplification are then analyzed by capillary electrophoresis.
Lan-Szu, Chou, Elaine, Lyon, Rong, Mao
openaire   +2 more sources

Methylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA)

2017
This chapter describes a method for the rapid assessment of promoter hypermethylation levels or methylation of imprinted regions in human genomic DNA extracted from various sources using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA).
Moelans, Cathy B., Atanesyan, Lilit, Savola, Suvi P, van Diest, Paul J.   +3 more
openaire   +3 more sources

Multiplex Ligation-dependent Probe Amplification (MLPA)

2011
The Multiplex Ligation-dependent Probe Amplification (MLPA) is a PCR-based method. The procedure relies on sequence-specific probe hybridization of genomic DNA, followed by multiplex-PCR amplification of the hybridized probe and a semiquantitative analysis of the resulting PCR products.
Ana Pilar Berbegall, Eva Villamón, Samuel Navarro, Rosa Noguera   +3 more
openaire   +1 more source

Multiplex Ligation-dependent Probe Amplification (MLPA) in Tumor Diagnostics and Prognostics

Diagnostic Molecular Pathology, 2012
The increasing knowledge about genetic alterations and molecular biomarkers in cancer initiation and progression opens new possibilities for the treatment of various types of cancer. This requires the inclusion of sensitive, and preferably multiplex, methods for the detection of molecular genetic alterations in the toolbox of classic pathology ...
Cornelia, Hömig-Hölzel, Suvi, Savola
openaire   +2 more sources

Comparison of Multiplex Ligation-Dependent Probe Amplification and Karyotyping in Prenatal Diagnosis

Obstetrics & Gynecology, 2010
To estimate whether multiplex ligation-dependent probe amplification (MLPA), a molecular technique used for detecting the most common chromosomal aneuploidies, is comparable with karyotyping for the detection of aneuploidies of chromosomes X, Y, 13, 18, and 21 in routine clinical practice and to estimate the costs differences of both techniques.In this
Boormans, Elisabeth M., Birnie, Erwin, Oepkes, Dick, Galjaard, Robert Jan, Schuring-Blom, Gijsbertha H., van Lith, Jan M., Hoffer, Mariëtte J., Macville, Merryn V., Knegt, Alida C., Smits, Arie, Wildschut, Hajo I., Go, Attie T., Bilardo, Catharina M., Bhola, Shama L., Nijhuis, Jan G., Creemers, Johan, Bouman, Katelijne, Suijkerbuijk, Ron, van Oppen, Adriana C., Bonsel, Gouke J.   +19 more
openaire   +8 more sources

Precise Expression Profiling by Stuffer-Free Multiplex Ligation-Dependent Probe Amplification

Analytical Chemistry, 2013
In systems biological studies, precise expression profiling of functionally important gene sets is crucial. Real-time polymerase chain reaction is generally used for this purpose. Despite its widespread acceptance, however, this method is not suitable for multiplex analysis, resulting in an inefficient assay process.
Shin, Gi Won, Na, Jeongkyeong, Seo, Mihwa, Chung, Boram, Nam, Hong Gil, Lee, Seung-Jae, Jung, Gyoo Yeol   +6 more
openaire   +4 more sources

Multiplex ligation-dependent probe amplification improves diagnostics in spinal muscular atrophy

Neuromuscular Disorders, 2006
Spinal muscular atrophy (SMA) is an autosomal recessive disease caused by decreased levels of survival motor neuron protein (SMN). In the majority of cases, this decrease is due to absence of the SMN1 gene. Multiplex ligation-dependent probe amplification (MLPA) is a modern quantitative molecular method. Applied in SMA cases, it improves diagnostics by
Eva L, Arkblad, Niklas, Darin, Kerstin, Berg, Eva, Kimber, Göran, Brandberg, Christopher, Lindberg, Eva, Holmberg, Mar, Tulinius, Margareta, Nordling   +8 more
openaire   +2 more sources