Results 81 to 90 of about 29,886 (297)

Quantitative methods to monitor RNA biomarkers in myotonic dystrophy [PDF]

, 2018
Myotonic dystrophy type 1 (DM1) and type 2 (DM2) are human neuromuscular disorders associated with mutations of simple repetitive sequences in afected genes.
A Ketley, A Mankodi, A Mankodi, A Perfetti, AE Gudde, AO Nygren, BJ Hindson, CL Liquori, E Eldering, J Liu, JD Brook, JF Huggett, JF Huggett, JP Schouten, K Klonowska, KL Taneja, LJ Sznajder, M DeJesus-Hernandez, M Goodwin, M Marcinkowska-Swojak, M Marcinkowska-Swojak, M Nakamori, M Rozanska, M Wojciechowska, M Wojciechowska, M Wojciechowska, MG Hamshere, P Kozlowski, R Batra, RA White 3rd, RB Siboni   +30 more
core   +1 more source

CDK4/6 Inhibition Induces CD8+ T Cell Antitumor Immunity via MIF‐Induced Functional Orchestration of Tumor‐Associated Macrophages

Advanced Science, EarlyView.
CDK4/6 inhibition promotes CD8+ T cell expansion through tumor‐macrophage crosstalk by activating HIF‐1α and enhancing MIF‐CD44/CD74 signaling. This reprograms TAMs to boost MHC‐I antigen presentation, and CDK4/6 inhibitor‐trained M1 TAM supernatant therapy synergizes with low‐dose PD‐1 blockade to restore antitumor immunity.
Lin He, Yuzhong Peng, Lat‐lun Leong, Jingbo Zhou, Dongyang Tang, Weilu Wang, Xiaoran Wu, Josh haipeng Lei, Yongqin Ye, Yangyang Feng, Yunfeng Qiao, Xiangpeng Chu, Di Mu, Qi Zhao, Tzuming Liu, Yan Chen, Paul Kwonghang Tam, Chu‐Xia Deng   +17 more
wiley   +1 more source

Accurate and effective multidrugresistant Mycobacterium tuberculosis detection method using gap-filling ligation coupled with high-resolution capillary electrophoresis-based single strand conformation polymorphism [PDF]

, 2017
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Cho, E., Choi, W., Jung, G.Y., Lee, J.
core   +1 more source

Rapid Decentralized Prostate Cancer Risk Stratification by Portable Liquid Biopsy Analysis within a Clinical Biosensor Validation Framework

Advanced Science, EarlyView.
In this work, we showcased two significant scientific advances in (i) developing a portable biosensor technology for rapid decentralized prostate cancer urinary biomarker testing and reporting, with superior performance to current clinical testing practice; and (ii) proposing a clinical validation framework (for our biosensor development) which can be ...
Kevin M. Koo, Grant Phillips, Sriganesh Srihari, Áine Farrell, Binny Jaradi, Kira J. Fitzpatrick, John W. Yaxley, Hemamali Samaratunga, Paul N. Mainwaring, Ke‐lin Ru, Darren J. Korbie, Scott A. Tomlins, Matthew J. Roberts, Robert A. Gardiner, Matt Trau   +14 more
wiley   +1 more source

Multiplex ligation dependent probe amplification - A useful, fast and cost-effective method for identification of small supernumerary marker chromosome in children with developmental delay and congenital heart defect

Romanian Journal of Laboratory Medicine, 2018
Small supernumerary marker chromosome (sSMC) is a rare chromosomal abnormality and is detected in about 0.3% in cases with multiple congenital anomalies (MCA) and/or developmental delay.
Crauciuc George Andrei, Tripon Florin, Bogliş Alina, Făgărăşan Amalia, Bănescu Claudia   +4 more
doaj   +1 more source

Genetic diagnosis as a tool for personalized treatment of Duchenne muscular dystrophy [PDF]

, 2016
Accurate definition of genetic mutations causing Duchenne muscular dystrophy (DMD) has always been relevant in order to provide genetic counseling to patients and families, and helps to establish the prognosis in the case where the distinction between ...
Bello, Luca, Pegoraro, Elena
core  

Quantitative analysis of the dystrophin gene by real-time PCR [PDF]

, 2012
Duchenne and Becker muscular dystrophy (DMD/BMD) are severe X-linked neuromuscular disorders caused by mutations in the dystrophin gene. Our aim was to optimize a quantitative real-time PCR method based on SYBR® Green I chemistry for routine ...
Anđelkovic Ana, Brankovic S., Bunjevacki Vera, Cvjeticanin Suzana, Damnjanovic Tatjana, Jekic Biljana, Kastratovic-Kotlica Biljana, Lukovic Ljiljana, Maksimovic Nela, Milic-Rasic Vedrana, Novakovic Ivana, Perovic Dijana, Rakocevic-Stojanovic Vidosava   +12 more
core   +2 more sources

Microglial Fkbp5 Impairs Post‐Stroke Vascular Integrity and Regeneration by Promoting Yap1‐Mediated Glycolysis and Oxidative Phosphorylation

Advanced Science, EarlyView.
A post‐stroke perivascular niche of microglia characterized by low expression of M2 markers and elevated glycolysis, oxidative phosphorylation (OXPHOS), and phagocytic activity is identified, which is termed stroke‐activated vascular‐associated microglia (stroke‐VAM).
Yanan Li, Yanmei Qiu, Yunlei Yang, Yanhao Wei, Haokun Peng, Longhai Zeng, Pengcheng Li, Rentang Bi, Bo Hu   +8 more
wiley   +1 more source

Design and Generation of MLPA Probe Sets for Combined Copy Number and Small-Mutation Analysis of Human Genes: EGFR as an Example

The Scientific World Journal, 2010
Multiplex ligation-dependent probe amplification (MLPA) is a multiplex copy number analysis method that is routinely used to identify large mutations in many clinical and research labs.
Malgorzata Marcinkowska, Kwok-Kin Wong, David J. Kwiatkowski, Piotr Kozlowski   +3 more
doaj   +1 more source

Contribution of Gli1+ Adventitial Stem Cells to Smooth Muscle Cells in Atherosclerosis and Vascular Injury

Advanced Science, EarlyView.
Gli1+ adventitial stem cells (ASCs) have been thought to generate smooth muscle cells (SMCs) in atherosclerosis. Using a dual‐recombinase lineage tracing to exclude ectopic labeling, Wang et al. found that Gli1+ ASCs do not contribute to SMCs in atherosclerotic plaques.
Haixiao Wang, Xiuzhen Huang, Jialing Mou, Enci Wang, Yan Li, Wenjuan Pu, Yao Xie, Xiaoling Guo, Lixin Wang, Maoping Chu, Bin Zhou, Chao Niu   +11 more
wiley   +1 more source